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Glycerol decreases the integrity of the perinuclear theca in boar sperm

  • Marco Antonio Arenas Núñez (a1), María de Lourdes Juárez-Mosqueda (a1), Oscar Gutiérrez-Pérez (a2), Santiago René Anzaldúa Arce (a3), Alejandro Córdova Izquierdo (a4), Roberto Martínez Rodríguez (a5) and María Elena Trujillo Ortega (a6)...


We evaluated the effect of glycerol on the perinuclear theca (PT) of boar sperm. Samples from six ejaculates obtained from three different boars were incubated in the detergent Brij 36-T. Spermatozoa were treated with a glycerol concentration of either 2 or 4%, and incubated for 10 or 30 min; two other samples were treated with protease inhibitors (PI; leupeptin or an inhibitor commercial cocktail), mixed with 4% glycerol, and incubated for 30 min. A third glycerol-free group was used as the control. The samples were processed for electron microscopy evaluation. The PT remained intact in 78% of the control samples while, after addition of glycerol for 30 min, the proportion of spermatozoa with disrupted or absent PT increased (P < 0.05). PT was preserved in PI samples, but PT changes increased (P < 0.05). Differences due to treatment with glycerol (2 or 4%) at 10 or 30 min were not observed. These results show, to our knowledge for the first time, the adverse effect of glycerol on the integrity of the PT.


Corresponding author

All correspondence to: Santiago René Anzaldúa Arce. Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, 04510, México, D.F. México, Tel:/Fax: +5255 5622 5893. e-mail:


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Glycerol decreases the integrity of the perinuclear theca in boar sperm

  • Marco Antonio Arenas Núñez (a1), María de Lourdes Juárez-Mosqueda (a1), Oscar Gutiérrez-Pérez (a2), Santiago René Anzaldúa Arce (a3), Alejandro Córdova Izquierdo (a4), Roberto Martínez Rodríguez (a5) and María Elena Trujillo Ortega (a6)...


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