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Expression of INHβA and INHβB proteins in porcine oocytes cultured in vitro is dependent on the follicle size

  • Bartosz Kempisty (a1) (a2) (a3), Hanna Piotrowska (a4), Marta Rybska (a5), Magdalena Woźna (a4), Paweł Antosik (a5), Dorota Bukowska (a5), Piotr Zawierucha (a2) (a3), Sylwia Ciesiółka (a2), Jędrzej M. Jaśkowski (a5), Michał Nowicki (a2), Klaus-Peter Brüssow (a6) and Maciej Zabel (a2)...

Summary

The current study aimed to investigate differential expression of inhibin βA (INHβA) and inhibin βB (INHβB) in porcine oocytes before or after in vitro maturation (IVM) isolated from follicles of various sizes. Porcine oocytes isolated from large, medium and small follicles (40 from each) were used to study the INHβA and INHβB protein expression pattern using western blot analysis before or after 44 h of oocyte IVM. An increased expression of INHβA was found in oocytes collected from large and medium follicles compared with small follicles before or after IVM (P < 0.001, P < 0.05, respectively). Similarly, higher INHβB levels were observed in oocytes recovered from large follicles compared with small (P < 0.01). As INHβA and INHβB are expressed in both porcine follicular somatic cells and oocytes, it can be assumed that these transforming growth factor beta (TGFβ) superfamily factors are involved in the regulation of molecular bi-directional pathways during follicle and oocyte development, and can be recognized as markers of follicle and oocyte maturation. Moreover, the current study clearly demonstrated that inhibin expression is substantially associated with porcine follicle growth and development.

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The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution-NonCommercial-ShareAlike licence <http://creativecommons.org/licenses/by-nc-sa/3.0/>. The written permission of Cambridge University Press must be obtained for commercial re-use.

Corresponding author

All correspondence to: Bartosz Kempisty. Department of Histology and Embryology, Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland. Tel: +48 61 854 6419. Fax: +48 61 854 6455. e-mail: etok@op.pl

References

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Expression of INHβA and INHβB proteins in porcine oocytes cultured in vitro is dependent on the follicle size

  • Bartosz Kempisty (a1) (a2) (a3), Hanna Piotrowska (a4), Marta Rybska (a5), Magdalena Woźna (a4), Paweł Antosik (a5), Dorota Bukowska (a5), Piotr Zawierucha (a2) (a3), Sylwia Ciesiółka (a2), Jędrzej M. Jaśkowski (a5), Michał Nowicki (a2), Klaus-Peter Brüssow (a6) and Maciej Zabel (a2)...

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