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Effects of oxygen concentrations on developmental competence and transcriptomic profile of yak oocytes

Published online by Cambridge University Press:  10 August 2020

Ruizhe Li Open the ORCID record for Ruizhe Li [Opens in a new window] ,
Yuzhu Luo ,
Jingtao Xu ,
Yonggang Sun ,
Zhijie Ma  and
Shengmei Chen
Ruizhe Li
Affiliation:
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, Gansu, China Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University,Xining, 810016, Qinghai, China
Yuzhu Luo*
Affiliation:
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, Gansu, China
Jingtao Xu
Affiliation:
Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University,Xining, 810016, Qinghai, China
Yonggang Sun
Affiliation:
Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University,Xining, 810016, Qinghai, China
Zhijie Ma
Affiliation:
Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University,Xining, 810016, Qinghai, China
Shengmei Chen
Affiliation:
Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University,Xining, 810016, Qinghai, China
*
Author for correspondence: Yuzhu Luo. Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, Gansu, China. E-mail: luoyz@gsau.edu.cn
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Summary

Oxygen concentration influences oocyte quality and subsequent embryo development, but it remains unclear whether oxygen concentrations affect the developmental competence and transcriptomic profile of yak oocytes. In this study, we investigated the effects of different oxygen concentrations (5% versus 20%) on the developmental competence, reactive oxygen species (ROS) levels, glutathione (GSH) content, and transcriptomic profile of yak oocytes. The results showed that a low oxygen concentration significantly increased the maturation rate of yak oocytes (81.2 ± 2.2% vs 75.9 ± 1.3%) and the blastocyst quality of yak in vitro fertilized embryos. Analysis of ROS and GSH showed that a low oxygen concentration reduced ROS levels and increased the content of GSH (75.05 ± 7.1 ng/oocyte vs 50.63 ± 5.6 ng/oocyte). Furthermore, transcriptomic analysis identified 120 differentially expressed genes (DEGs) between the two groups of oocytes. Gene enrichment analysis of the DEGs indicated multiple cellular processes, including oxidative phosphorylation, transcription regulation, mitochondrial regulation, oestrogen signalling pathway, HIF-1 signalling pathway, TNF signalling pathway, were involved in the response to oxygen concentration alterations. Taken together, these results indicated that a low oxygen concentration improved the developmental competence of yak oocytes.

Keywords

Developmental competenceOocyteOxygen concentrationsTranscriptomeYak
Type
Research Article
Information
Zygote , Volume 28 , Issue 6 , December 2020 , pp. 459 - 469
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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