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Effects of high calcium levels on the disturbed extrusion of the second polar body during in vitro fertilization in C3H/He mouse substrains

  • Y. Ohta-Takada (a1) (a2) (a3), Y. Nagao (a2) and S. Kito (a3)

Summary

We previously reported that high concentrations (≥3.42 mM) of calcium during in vitro fertilization (IVF) disturbed the extrusion of the second polar body (PBII) in C3H/He inbred mice. In this study, the substrain specificity of this phenomenon was examined under 1.71–6.84 mM calcium concentration in ova from six C3H/He mouse commercially available substrains in Japan. PBII extrusion in ova from J substrains was not affected by calcium concentrations (<10% at any calcium level), but was grossly disturbed at high calcium levels in the ova of other substrains. This result has practical applications for the efficient production of normal zygotes by IVF, therefore contributing to the reduction in the numbers of donor animals for further zygote or embryo manipulation. Care must be taken in choosing IVF medium for particular strains and substrains.

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Corresponding author

Author for correspondence: Seiji Kito, Centre of Animal Research and Education, Nagoya University, Furo-cho Chikusa, Nagoya, 464–8601, Japan. Tel: +81 52 747 6875. Fax: +81 52 747 6874. E-mail: sk126@care.nagoya-u.ac.jp

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Present address: Yakult Central Institute, Tokyo, Japan.

Present address: Centre of Animal Research and Education, Nagoya University, Nagoya, Japan.

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References

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Keywords

Effects of high calcium levels on the disturbed extrusion of the second polar body during in vitro fertilization in C3H/He mouse substrains

  • Y. Ohta-Takada (a1) (a2) (a3), Y. Nagao (a2) and S. Kito (a3)

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