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Effect of zinc chloride and sodium selenite supplementation on in vitro maturation, oxidative biomarkers, and gene expression in buffalo (Bubalus bubalis) oocytes

Published online by Cambridge University Press:  26 March 2021

Wael A. Khalil ,
Chun-Yan Yang ,
Mostafa M. El-Moghazy ,
Mohamed S. El-Rais ,
Jiang-Hua Shang  and
Ashraf El-Sayed Open the ORCID record for Ashraf El-Sayed [Opens in a new window]
Wael A. Khalil*
Affiliation:
Animal Production Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt
Chun-Yan Yang
Affiliation:
Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture and Guangxi, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, 530001, China
Mostafa M. El-Moghazy
Affiliation:
Animal Production Department, Faculty of Agriculture, Damietta University, Damietta, Egypt
Mohamed S. El-Rais
Affiliation:
Animal Production Department, Faculty of Agriculture, Damietta University, Damietta, Egypt
Jiang-Hua Shang
Affiliation:
Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture and Guangxi, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, 530001, China
Ashraf El-Sayed
Affiliation:
Animal Production Department, Faculty of Agriculture, Cairo University, Giza, Egypt King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
*
Author for correspondence: Wael A. Khalil. Animal Production Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt. E-mail: w-khalil@mans.edu.eg
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Summary

This study examined the effects of zinc chloride (ZnCl2) and sodium selenite (Na2SeO3) supplementation in maturation medium on in vitro maturation (IVM) rate, oxidative biomarkers and gene expression in buffalo oocytes. Ovaries from a slaughterhouse were aspirated and good quality cumulus–oocyte complexes (COCs) with at least four layers of compact cumulus cells and evenly granulated dark ooplasm were selected. COCs were randomly allocated during IVM (22 h) to one of four treatment groups: (1) control maturation medium (basic medium), or basic medium supplemented with (2) ZnCl2 (1.5 µg/ml), (3) Na2SeO3 (5 µg/l), or (4) ZnCl2 + Na2SeO3 (1.5 µg/ml + 5 µg/l, respectively). Oocytes were denuded after 22 h of IVM in the first four replicates. Specimens were fixed and stained to evaluate the stage of nuclear maturation. The spent medium was collected for biochemical assays of total antioxidant capacity (TAC), malondialdehyde (MDA) and hydrogen peroxide concentrations. A second four replicates were used for COCs for RNA extraction. The expression levels of antioxidant (SOD1, GPX4, CAT and PRDX1), antiapoptotic (BCL2 and BCL-XL) and proapoptotic (BAX and BID) genes were measured. Supplementation with ZnCl2 and Na2SeO3 during IVM increased the ratio of oocytes reaching metaphase II at 22 h, increased TAC and decreased MDA and H2O2 concentrations in the maturation medium (P < 0.05). Moreover, beneficial effects were associated with complementary changes in expression patterns of antioxidative, antiapoptotic and proapoptotic genes, suggesting lower oxidative stress and apoptosis. Supplementation medium with zinc chloride and sodium selenite improves the maturation rate, reduces oxidative stress and increases expression levels of antioxidative and antiapoptotic genes.

Keywords

BuffaloGene expressionIn vitro maturationOxidative biomarkersSodium seleniteZinc chloride
Type
Research Article
Information
Zygote , Volume 29 , Issue 5 , October 2021 , pp. 393 - 400
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this work.

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