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The effect of growth hormone (GH) and insulin-like growth factor-I (IGF-I) on in vitro maturation of equine oocytes

Published online by Cambridge University Press:  28 July 2011

Gabriel Ribas Pereira ,
Pedro Luis Lorenzo ,
Gustavo Ferrer Carneiro ,
Barry Allen Ball ,
Paulo Bayard Dias Gonçalves ,
Lígia Maria Cantarelli Pegoraro ,
Sylvie Bilodeau-Goeseels ,
John P. Kastelic ,
Patrick J. Casey  and
Irwin K. M. Liu
Gabriel Ribas Pereira*
Affiliation:
Laboratory of Biotechnology and Animal Reproduction–BioRep, Federal University of Santa Maria, Ave. Roraima #1000, CEP: 97105–900, Santa Maria, RS, Brazil. Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, USA.
Pedro Luis Lorenzo
Affiliation:
Departamento de Fisiología Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
Gustavo Ferrer Carneiro
Affiliation:
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, USA.
Barry Allen Ball
Affiliation:
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, USA.
Paulo Bayard Dias Gonçalves
Affiliation:
Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, Brazil.
Lígia Maria Cantarelli Pegoraro
Affiliation:
Animal Reproduction Laboratory, EMBRAPA Temperate Climate Research Corporation, Pelotas, Brazil.
Sylvie Bilodeau-Goeseels
Affiliation:
Agriculture and Agri-Food Canada Research Centre, Lethbridge, Canada.
John P. Kastelic
Affiliation:
Agriculture and Agri-Food Canada Research Centre, Lethbridge, Canada.
Patrick J. Casey
Affiliation:
Research Centre in Reproductive Medicine, University of Auckland, Auckland, New Zealand.
Irwin K. M. Liu
Affiliation:
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, USA.
*
All correspondence to: Gabriel Ribas Pereira. Laboratory of Biotechnology and Animal Reproduction–BioRep, Federal University of Santa Maria, Ave. Roraima #1000, CEP: 97105–900, Santa Maria, RS, Brazil. Tel: +55 55 3220–8752. Fax: +55 55 3220 8484. E-mail: gabrielrp@biorep.ufsm.br or grpereira@vmth.ucdavis.edu
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Summary

The objective of this study was to test the hypothesis that equine growth hormone (eGH), in combination with insulin growth factor-I (IGF-I), influences positively in vitro nuclear and cytoplasmic maturation of equine oocytes. Cumulus–oocyte complexes were recovered from follicles that were < 25 mm in diameter, characterized by morphology and were allocated randomly as follow: (a) control (no additives); (b) 400 ng/ml eGH; (c) 200 ng/ml IGF-I; (d) eGH + IGF-I; and (e) eGH + IGF-I + 400 ng/ml anti-IGF-I antibody. Oocytes were matured for 30 h at 38.5°C in air with 5% CO2 and then stained with 10 μg/ml propidium iodide (PI) to evaluate nuclear status and 10 μg/ml Lens culinaris agglutinin-fluorescein complex (FITC-LCA) to assess cortical granule migration by confocal microscopy. The proportion of immature oocytes that developed to the metaphase II (MII) stage in the eGH + IGF-I group (15 of 45) was greater than in the groups that were treated only with IGF-I (7 of 36, p = 0.03). Oocytes that reached MII in the control group (20 of 56; 35.7%) showed a tendency to be different when compared with eGH + IGF-I group (15 of 45; 33.3%, p = 0.08). The treated group that contained anti-IGF-I (15 of 33; 45.4%) decreased the number of oocytes reaching any stage of development when compared with eGH (47 of 72; 65.3%) and eGH + IGF-I (33 of 45; 73.3%) groups (p = 0.05) when data from MI and MII were combined. We concluded that the addition of eGH to in vitro maturation (IVM) medium influenced the in vitro nuclear and cytoplasmic maturation of equine oocytes. The use of GH and IGF-I in vitro may represent a potential alternative for IVM of equine oocytes.

Keywords

Cortical granuleseGHEquine oocyteIGF-INuclear and cytoplasmic maturation
Type
Research Article
Information
Zygote , Volume 20 , Issue 4 , November 2012 , pp. 353 - 360
Copyright
Copyright © Cambridge University Press 2011

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