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Effect of embryonic cell cycle of nuclear donor embryos on the efficiency of nuclear transfer in Japanese black cattle

  • M. Kishi (a1) (a2), R. Takakura (a1), Y. Nagao (a1), K. Saeki (a1) and Y. Takahashi (a2)...

Summary

In the present study, the development in vitro and in vivo of nuclear transfer (NT) embryos reconstructed with embryonic cells (blastomeres) at the 32- to 63-cell (sixth cell cycle) and 64- to 127-cell (seventh cell cycle) stages was investigated to determine the optimum range of embryonic cell cycles for yielding the highest number of identical calves in Japanese black cattle. Rates of development to the blastocyst stage (overall efficiency) were higher in the sixth cell-cycle stage (45%) than in the seventh cell-cycle stage (12%). After the transfer of the blastocysts reconstructed with blastomeres of the sixth and seventh cell cycle-stage embryos to recipient heifers, there were no differences in the pregnancy (14/35: 40% versus 3/13: 23%, respectively) or calving rates (11/39: 28% versus 3/13: 23%, respectively). These results indicate that the highest number of identical calves would be obtained by using sixth cell cycle (32- to 63-cell)-stage embryos as nuclear donors.

Copyright

Corresponding author

All correspondence to: Yoshiyuki Takahashi, Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan. Tel: +81 11 706 5231. Fax: +81 11 706 5232. e-mail: ytaka@vetmed.hokudai.ac.jp

References

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Barnes, F., Collas, P., Powel, R., King, W.A., Westhusin, M. & Shepherd, D. (1993). Influence of recipient oocytes cell stage on DNA synthesis, nuclear envelope breakdown, chromosome constitution, and development in nuclear transfer bovine embryos. Mol. Reprod. Dev. 36, 3341.
Bavister, B.D., Leibfried, M.L. & Lieberman, G. (1983). Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol. Reprod. 28, 235–47.
Bondioli, K.R. (1993). Nuclear transfer in cattle. Mol. Reprod. Dev. 36, 274–5.
Bondioli, K.R., Westhusin, M.E. & Looney, C.R. (1990). Production of identical bovine offspring by nuclear transfer. Theriogenology 33, 165–74.
Bourhis, D.L., Chesne, P., Nibart, M., Marchal, J., Humblot, P., Renard, J.P. & Heyman, Y. (1998). Nuclear transfer from sexed parent embryos in cattle: efficiency and birth of offspring. J. Reprod. Fertil. 113, 343–8.
Campbell, K.H.S., Loi, P., Cappai, P. & Wilmut, I. (1994). Improved development to blastocyst of ovine nuclear transfer embryos reconstructed during the presumptive S-phase of enucleated activated oocytes. Biol. Reprod. 50, 1385–93.
Campbell, K.H., Loi, P., Otaegui, P. & Wilmut, I. (1996). Cell cycle coordination in embryo cloning by nuclear transfer. Rev. Reprod. 1, 40–6.
Cheong, H.T., Takahashi, Y. & Kanagawa, H. (1993). Birth of mice after transplantation of early cell-cycle stage embryonic nuclei into enucleated oocytes. Biol. Reprod. 48, 958–63.
First, N.L., Leibfried-Rutledge, M.L., Northey, D.L. & Nuttleman, P.R. (1992). Use of in vitro matured oocytes 24 hr of age in bovine nuclear transfer. Theriogenology 37, 211.
Garry, F.B., Adams, R., McCann, J.P. & Odde, K.G. (1996). Postnatal characteristics of calves produced by nuclear transfer cloning. Theriogenology 45, 141–52.
Handyside, A.H. & Johnson, M.H. (1978). Temporal and spatial pattern of the synthesis of tissue-specific polypeptides in the preimplantation mouse embryo. J. Embryol. Exp. Morphol. 44, 191–9.
Heyman, Y., Chavatte-Palmer, P., LeBourhis, D., Camous, S., Vignon, X. & Renard, J.P. (2002). Frequency and occurrence of late-gestation losses from cattle cloned embryos. Biol. Reprod. 66, 613.
Hirooka, H. (2000). Evaluation of testing schemes with clones for carcass traits in beef cattle. Anim. Sci. J. 71, j19j25 (in Japanese).
Kruip, T.A.M. & den Daas, J.H.G. (1997). In vitro produced and cloned embryos: effects on pregnancy, parturition and offspring. Theriogenology 47, 4352.
Kuchida, K., Ogasawara, Y., Hidaka, S., Minamihashi, A., Sakai, T. & Yamamoto, Y. (2003). Computer image analysis on similarity of embryonic clones to beef carcass cross section. Anim. Sci. J. 74, 17 (in Japanese).
Mitani, T., Utsumi, K. & Iritani, A. (1993). Developmental ability of enucleated bovine oocytes matured in vitro after fusion with single blastomeres of eight-cell embryos matured and fertilized in vitro. Mol. Reprod. Dev. 34, 314–22.
Nagao, Y., Saeki, K., Hoshi, M. & Nagai, M. (1995). Early development of bovine embryos. J. Reprod. Dev. 41, j29j36 (in Japanese).
Newcomb, R., Rowson, L.E.A. & Trounson, A.O. (1976). The entry of superovulated eggs into uterus. In: Egg Transfer in Cattle (ed. L.E.A. Rowson), pp. 115. Commission of European Communities, Luxembourg.
Renard, J.P., Zhou, Q., LeBourhis, D., Chavatte-Palmer, P., Hue, I., Heyman, Y. & Vignon, X. (2002). Nuclear transfer technologies: between successes and doubts. Theriogenology 57, 203–22.
Ritchie, W.A. & Campbell, K.H.S. (1995). Intracytoplasmic nuclear injection as an alternative to cell fusion for the production of embryos by nuclear transfer. J. Reprod. Fertil. 15, 60.
Saeki, K., Hoshi, M., Leibfried-Rutledge, M.L. & First, N.L. (1990). In vitro fertilization and development of bovine oocytes matured with commercially available follicle stimulating hormone. Theriogenology 34, 1035–9.
Sims, M. & First, N.L. (1994). Production of calves by transfer of nuclei from cultured inner cell mass cells. Proc. Natl. Acad. Sci. USA 91, 6143–7.
Smith, L.C. & Wilmut, I. (1989). Influence of nuclear and cytoplasmic activity on the development in vivo of sheep embryos after nuclear transplantation. Biol. Reprod. 40, 1027–35.
Smith, S.L., Everts, R.E., Tian, X.C., Du, F., Sung, L.Y., Rodriguez-Zas, S.L., Jeong, B.S., Renard, J.P., Lewin, H.A. & Yang, X. (2005). Global gene expression profiles reveal significant nuclear reprogramming by the blastocyst stage after cloning. Proc. Natl. Acad. Sci. USA 102, 17582–7.
Stice, S.L. & Keefer, C.L. (1993). Multiple generational bovine embryo cloning. Biol. Reprod. 48, 715–9.
Takahashi, Y. & First, N.L. (1992). In vitro development of bovine one-cell embryos: influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenology 37, 963–78.
Tani, T., Kato, Y. & Tsunoda, Y. (2001). Direct exposure of chromosomes to nonactivated ovum cytoplasm is effective for bovine somatic cell nucleus reprogramming. Biol. Reprod. 64, 324–30.
Tervit, H.R., Whittingham, D.G. & Rowson, L.E. (1972). Successful culture in vitro of sheep and cattle ova. J. Reprod. Fertil. 30, 493–7.
Ushijima, H., Tsunoda, Y., Eto, T. & Imai, H. (1991). In vitro development of bovine reconstituted eggs after fusion with a blastomere from 8-cell to blastocyst stage embryos. Jpn. J. Anim. Reprod. 37, 15–9 (in Japanese).
Wilmut, I., Beaujean, N., de Sousa, P.A., Dinnyes, A., King, T.J., Paterson, L.A., Wells, D.N. & Young, L.E. (2002). Somatic cell nuclear transfer. Nature 419, 583–6.
Wilson, J.M., Williams, J.D., Bondioli, K.R., Looney, C.R., Westhusin, M.E. & McCalla, D.F. (1995). Comparison of birth weight and growth characteristics of bovine calves produced by nuclear transfer (cloning), embryo transfer and natural mating. Anim. Reprod. Sci. 38, 7383.
Wolfe, B.A. & Kraemer, D.C. (1992). Methods in bovine nuclear transfer. Theriogenology 37, 515.
Yang, X.Z., Jiang, S., Farrell, P., Foote, R.H. & McGrath, A.B. (1993). Nuclear transfer in cattle: effect of nuclear donor cells, cytoplast age, co-culture, and embryo transfer. Mol. Reprod. Dev. 35, 2936.
Zakhartchenko, V., Reichenbach, H.D., Riedl, J., Palma, G.A., Wolf, E. & Brem, G. (1996). Nuclear transfer in cattle using in vivo-derived versus in vitro-produced donor embryos: effect of developmental stage. Mol. Reprod. Dev. 44, 493–8.

Keywords

Effect of embryonic cell cycle of nuclear donor embryos on the efficiency of nuclear transfer in Japanese black cattle

  • M. Kishi (a1) (a2), R. Takakura (a1), Y. Nagao (a1), K. Saeki (a1) and Y. Takahashi (a2)...

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