Campelo, IS, Pereira, AF, Alcântara-Neto, AS, Canel, NG, Souza-Fabjan, JMG, Teixeira, DIA, Camargo, LS, Melo, LM, Rádis-Baptista, G, Salamone, DF and Freitas, VJF (2016a) Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos. Zygote 24, 48–57.
Campelo, IS, Canel, NG, Bevacqua, RJ, Melo, LM, Rádis-Baptista, G, Freitas, VJF and Salamone, DF (2016b) Crotamine, a cell-penetrating peptide, is able to translocate parthenogenetic and in vitro fertilized bovine embryos but does not improve exogenous DNA expression. J Assist Reprod Genet 33, 1405–13.
Campos, VF, de Leon, PM, Komninou, ER, Dellagostin, OA, Deschamps, JC, Seixas, FK and Collares, T (2011) NanoSMGT: transgene transmission into bovine embryos using halloysite clay nanotubes or nanopolymer to improve transfection efﬁciency. Theriogenology 76, 1552–60.
Carballada, R, Relloso, M and Esponda, P (2002) Generation of transgenic mice by transfection of pronuclear embryos using lipid–DNA complexes. Zygote 10, 209–16.
Chang, K, Qian, J, Jiang, M, Liu, YH, Wu, MC, Chen, CD, Lai, CK, Lo, HL, Hsiao, CT, Brown, L, Bolen, J Jr, Huang, HI, Ho, PY, Shih, PY, Yao, CW, Lin, WJ, Chen, CH, Wu, FY, Lin, YJ, Xu, J and Wang, K (2002) Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer. BMC Biotechnol 2, 5.
Chen, PC, Hayashi, MAF, Oliveira, EB and Karpel, RL (2012) DNA-interactive properties of crotamine, a cell-penetrating polypeptide and a potential drug carrier. PLoS One 7, e48913.
Ebert, KM, Selgrath, JP, DiTullio, P, Denman, J, Smith, TE, Memon, MA, Schindler, JE, Monastersky, GM, Vitale, JA and Gordon, K (1991) Transgenic production of a variant of human tissue-type plasminogen activator in goat milk: generation of transgenic goats and analysis of expression. Biotechnology 9, 835–8.
Fadel, V, Bettendorff, P, Herrmann, T, de Azevedo, WF , Jr, Oliveira, EB, Yamane, T and Wüthrich, K (2005) Automated NMR structure determination and disulfide bond identification of the myotoxin crotamine from Crotalus durissus terrificus
. Toxicon 46, 759–767.
Freitas, VJF, Alcântara-Neto, AS, Pereira, AF, Campelo, IS, Melo, LM and Rádis-Baptista, G (2014) Assessing the complex formation between crotamine – a natural cell penetrating peptide – and DNA using high sensitive fluorescence exclusion assay. Clon Transgen 3, 128.
Garrels, W, Ivics, Z and Kues, WA (2012) Precision genetic engineering in large mammals. Trends Biotechnol 30, 386–93
Gonçalves, JM and Polson, A (1947) The electrophoretic analysis of snake venoms. Arch Biochem 13, 253–9.
Gordon, JW, Scangos, GA, Plotkin, DJ, Barbosa, JA and Ruddle, FH (1980) Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci USA 77, 7380–4.
Hacker, DL and Balasubramanian, S (2016) Recombinant protein production from stable mammalian cell lines and pools. Curr Opin Struct Biol 38, 129–36.
Hammer, RE and Pursel, VG (1985) Production of transgenic rabbits, sheep and pigs by microinjection. Nature 315, 680–3.
Iqbal, K, Barg-Kues, B, Brol, S, Bode, J, Niemann, H and Kues, WA (2009) Cytoplasmic injection of circular plasmids allows targeted expression in mammalian embryos. Biotechniques 47, 959–68.
Jaenisch, R and Mintz, B (1974) Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA. Proc Natl Acad Sci USA 71, 1250–4.
Kerkis, A, Kerkis, I, Rádis-Baptista, G, Oliveira, EB, Vianna-Morgante, AM, Pereira, LV and Yamane, T (2004) Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
. FASEB J 18, 1407–9.
Kim, JS (2016) Genome editing comes of age. Nat Protoc 11, 1573–8.
Krimpenfort, P, Rademakers, A, Eyestone, W, van der Schans, A, van den Broek, S, Kooiman, P, Kootwijk, E, Platenburg, G, Pieper, F, Strijker, R and de Boer, H (1991) Generation of transgenic dairy cattle using in vitro embryo production. Biotechnology 9, 844–7.
Kues, WA and Niemann, H (2011) Advances in farm animal transgenesis. Prev Vet Med 102, 146–56.
Li, R, Miao, J, Fan, Z, Song, S, Kong, IK, Wang, Y and Wang, Z (2018) Production of genetically engineered golden Syrian hamsters by pronuclear injection of the CRISPR/Cas9 complex. J Vis Exp 131, 56263.
Malaweera, DBO, Ramachandra, S, Wu, JB, Oh, SK, Kim, SH, Kim, SJ, Jang, G and Cho, JK (2014) Establishment of efficient microinjection system in the porcine embryos. J Emb Transf 29, 59–66.
Marinovic, MP, Dal Mas, C, Monte, GG, Felix, D, Campeiro, JD and Hayashi, MAF (2016) Crotamine: function diversity and potential applications. In Gopalakrishnakone, P, Inagaki, H, Mukherjee, A, Rahmy, T and Vogel, CW (eds) Snake venoms. Dordrecht: Springer.
Meng, F, Li, H, Wang, X, Qin, G, Oback, B and Shi, D (2015) Optimized production of transgenic buffalo embryos and offspring by cytoplasmic zygote injection. J Anim Sci Biotechnol 6, 44.
Nascimento, FD, Hayashi, MA, Kerkis, A, Oliveira, V, Oliveira, EB, Rádis-Baptista, G, Nader, HB, Yamane, T, Tersariol, IL and Kerkis, I (2007) Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans. J Biol Chem 282, 21349–60.
Nicastro, G, Franzoni, L, de Chiara, C, Mancin, AC, Giglio, JR and Spisni, A (2003) Solution structure of crotamine, a Na+ channel affecting toxin from Crotalus durissus terrificus venom. Eur J Biochem 270, 1969–79.
Pooga, M and Langel, Ü (2015) Classes of cell-penetrating peptides. Methods Mol Biol 1324, 3–28.
Rádis-Baptista, G and Kerkis, I (2011) Crotamine, a small basic polypeptide myotoxin from rattlesnake venom with cell-penetrating properties. Curr Pharm Des 17, 4351–61.
Rádis-Baptista, G, de la Torre, BG and Andreu, D (2008) A novel cell-penetrating peptide sequence derived by structural minimization of a snake toxin exhibits preferential nucleolar localization. J Med Chem 51, 7041–4.
Rádis-Baptista, G, de la Torre, BG and Andreu, D (2012) Insights into the uptake mechanism of NrTP, a cell-penetrating peptide preferentially targeting the nucleolus of tumour cells. Chem Biol Drug Des 79, 907–15.
Rádis-Baptista, G, Campelo, IS, Morlighem, JRL, Melo, LM and Freitas, VJF (2017) Cell-penetrating peptides (CPPs): from delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis. J Biotechnol 252, 15–26.
Rieth, A, Pothier, F and Sirard, MA (2000) Electroporation of bovine spermatozoa to carry DNA containing highly repetitive sequences into oocytes and detection of homologous recombination events. Mol Reprod Dev 57, 338–45.
Salamone, D, Barañao, L, Santos, C, Bussmann, L, Artuso, J, Werning, C, Prync, A, Carbonetto, C, Dabsys, S, Munar, C, Salaberry, R, Berra, G, Berra, I, Fernández, N, Papouchado, M, Foti, M, Judewicz, N, Mujica, I, Muñoz, L, Alvarez, SF, González, E, Zimmermann, J, Criscuolo, M and Melo, C (2006) High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow. J Biotechnol 124, 469–72.
Stewart, KM, Horton, KL and Kelley, SO (2008) Cell-penetrating peptides as delivery vehicles for biology and medicine. Org Biomol Chem, 6, 2242–55.
Vichera, G, Moro, L and Salamone, D (2011) Efficient transgene expression in IVF and parthenogenetic bovine embryos by intracytoplasmic injection of DNA–liposome complexes. Reprod Domest Anim 46, 214–20.
Xu, YN, Uhm, SJ, Koo, BC, Kwon, MS, Roh, JY, Yang, JS, Choi, HY, Heo, YT, Cui, XS, Yoon, JH, Ko, DH, Kim, T and Kim, NH (2013) Production of transgenic Korean native cattle expressing enhanced green fluorescent protein using a FIV-based lentiviral vector injected into MII oocytes. J Genet Genomics 40, 37–43.