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Discrimination of the effects on zebrafish reproduction from pollutants in drinking water via female, via male and/or via fecundation water

  • M. Martínez-Sales (a1), F. García-Ximénez (a2) and F.J. Espinós (a2)


The lack of preventive policy legislation and the low removal rate of organic pollutants in conventional potabilization treatments lead to some of them being present in drinking water. The problem arises because some of these substances have detrimental effects on human reproduction health, via females, via males or even both. In this work, we established the zebrafish as a bioindicator of these types of substances with the goal of discriminating the effects through three different pathways: male, female or water where the fertilization took place.

For this purpose, four parameters were analysed: fertility rate, hatching rate and survival and abnormalities rates. So, for each parameter two groups were formed, according to whether adult males or females were reared in bottled spring water (Z) or tap water (B) and if the in vitro fertilization took place in water Z or B.

Results revealed a decline in the fertility and hatching rate in water B, due to a water effect. The most plausible explanation could be the presence of substances which affect the micropyle and chorion. Moreover, a decrease in the fertility rate due to an effect over the female was also observed, but in this case by an alteration of the oocyte quality.


Corresponding author

All correspondence to: M. Martínez-Sales. Aquaculture and Environmental Research Group (ACUMA), Universidad Politécnica de Valencia, Camino de Vera 14, 46022, Valencia, Spain. Tel: +34963879433. E-mail:


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Bolong, N., Ismail, A.F., Salim, M.R. & Matsuura, T. (2009). A review of the effects of emerging contaminants in wastewater and options for their removal. Desalination 239, 229–46.
Brand, M., Granato, M. & Nüslein-Volhard, C. (2002). Keeping and raising zebrafish. In Zebrafish: A Practical Approach (eds Nüslein-Volhard, C. & Dahm, R.), pp. 733. Oxford, UK: Oxford University Press.
Braw-Tal, R. (2010). Endocrine disruptors and timing of human exposure. Pediatr. Endocrinol. Rev. 8, 41–6.
Galus, M., Kirischian, N., Higgins, S., Purdy, J., Chow, J., Rangaranjan, S., Li, H., Metcalfe, C. & Wilson, J.Y. (2013). Chronic, low concentration exposure to pharmaceuticals impacts multiple organ systems in zebrafish. Aquat. Toxicol. 132–133, 200–11.
Galus, M., Rangarajan, S., Lai, A., Shaya, L., Balshine, S. & Wilson, J.Y. (2014). Effects of chronic, parental pharmaceutical exposure on zebrafish (Danio rerio) offspring. Aquat. Toxicol. 151, 124–34.
Khetan, S.K. & Collins, T.J. (2007). Human pharmaceuticals in the aquatic environment: a challenge to green chemistry. Chem. Rev. 107, 2319–64.
Martínez-Sales, M., García-Ximénez, F. & Espinós, F.J. (2014). Zebrafish (Danio rerio) as a possible bioindicator of epigenetic factors present in drinking water that may affect reproductive function: is chorion an issue? Zygote 23, 447–52.
Martínez-Sales, M., García-Ximénez, F. & Espinós, F.J. (2015). Zebrafish as a possible bioindicator of organic pollutants with effects on reproduction in drinking waters. J. Environ. Sci. 33, 254–60.
Matthews, M., Trevarrow, B. & Matthews, J. (2002). A virtual tour of the Guide for zebrafish users. Lab. Anim. 31, 3440.
Powers, C.M., Yen, J., Linney, E.A., Seidler, F.J. & Slotkin, T.A. (2010). Silver exposure in developing zebrafish (Danio rerio): Persistent effects on larval behavior and survival. Neurotoxicol. Teratol. 32, 391–7.
Richardson, S.D. & Ternes, T.A. (2011). Water analysis: emerging contaminants and current issues. Anal Chem. 83, 4614–48.
Rodil, R., Quintana, J.B., Concha-Graña, E., López-Mahía, P., Muniategui-Lorenzo, S. & Prada-Rodríguez, D. (2012). Emerging pollutants in sewage, surface and drinking water in Galicia (NW Spain). Chemosphere 86, 1040–9.
Santos, E.M., Paull, G.C., Van Look, K.J., Workman, V.L., Holt, W.V., Van Aerle, R., Kile, P. & Tyler, C.R. (2007). Gonadal transcriptome responses and physiological consequences of exposure to oestrogen in breeding zebrafish (Danio rerio). Aquat. Toxicol. 83, 134–42.
Schriks, M., Heringa, M.B., van der Kooi, M.M., de Voogt, P. & van Wezel, A.P. (2010). Toxicological relevance of emerging contaminants for drinking water quality. Water Res. 44, 461–76.
Sharpe, R.M. & Irvine, D.S. (2004). How strong is the evidence of a link between environmental chemicals and adverse effects on human reproductive health? Brit. Med. J. 328, 447–51.
Shi, X., Du, Y., Lam, P., Wu, R. & Zhou, B. (2008). Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS. Toxicol. Appl. Pharmacol. 230, 2332.
Simão, M., Cardona-Costa, J., Pérez Camps, M. & García-Ximénez, F. (2010). Ultraviolet radiation dose to be applied in recipient zebrafish embryos for germ-line chimaerism is strain dependent. Reprod. Domest. Anim. 45, 1098–103.
Silva, E., Rajapakse, N. & Kortenkamp, A. (2002). Something from “nothing”—eight weak estrogenic chemicals combined at concentrations below NOECs produce significant mixture effects. Environ Sci Technol. 36, 1751–6.
Toft, G., Rignell-Hydbom, A., Tyrkiel, E., Shvets, M., Giwercman, A., Lindh, C.H., Pedersen, H.S., Ludwicki, J.K., Lesovoy, v., Hagmar, L., Spanó, M., Manicardi, G.C., Bonefeld-Jorgensen, E.C., Thulstrup, A.M. & Bonde, J.P. (2006). Semen quality and exposure to persistent organochlorine pollutants. Epidemiology 17, 450–8.
Vested, A., Giwercman, A., Bonde, J.P. & Toft, G. (2014). Persistent organic pollutants and male reproductive health. Asian J. Androl. 16, 7180.
Westerfield, M. (1995). The Zebrafish Book. Eugene, Oregon, USA: University of Oregon Press.
Westerfield, M. (2007). The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Danio rerio), 5th edn. Eugene, Oregon, USA: University of Oregon Press.
Zhu, X., Zhu, L., Duan, Z., Qi, R., Li, Y. & Lang, Y. (2008). Comparative toxicity of several metal oxide nanoparticle aqueous suspensions to zebrafish (Danio rerio) early developmental stage. J. Environ. Sci. Heal. A 43, 278–84.
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