Hostname: page-component-76fb5796d-skm99 Total loading time: 0 Render date: 2024-04-25T07:44:17.540Z Has data issue: false hasContentIssue false
  • English
  • Français

Digital holographic microscopy for the evaluation of human sperm structure

Published online by Cambridge University Press:  07 March 2013

G. Coppola ,
G. Di Caprio ,
M. Wilding ,
P. Ferraro ,
G. Esposito ,
L. Di Matteo ,
R. Dale ,
G. Coppola  and
B. Dale
G. Coppola*
Affiliation:
Centro Fecondazione Assistita (CFA-Italia), Via Manzoni 15, 80123 Naples, Italy.
G. Di Caprio
Affiliation:
Istituto per la Microelettronica e i Microsistemi, del Consiglio Nazionale delle Ricerche, Sezione di Napoli, Via P. Castellino, 111, 80131 Naples, Italy.
M. Wilding
Affiliation:
Centro Fecondazione Assistita (CFA-Italia), Via Manzoni 15, 80123 Naples, Italy.
P. Ferraro
Affiliation:
Istituto Nazionale di Ottica, del Consiglio Nazionale delle Ricerche, Sezione di Napoli, c/o Comprensorio Olivetti, Via Campi Flegrei 34 - 80078 Pozzuoli, Italy.
G. Esposito
Affiliation:
Centro Fecondazione Assistita (CFA-Italia), Via Manzoni 15, 80123 Naples, Italy.
L. Di Matteo
Affiliation:
Centro Fecondazione Assistita (CFA-Italia), Via Manzoni 15, 80123 Naples, Italy.
R. Dale
Affiliation:
Centro Fecondazione Assistita (CFA-Italia), Via Manzoni 15, 80123 Naples, Italy.
G. Coppola*
Affiliation:
Istituto per la Microelettronica e i Microsistemi, del Consiglio Nazionale delle Ricerche, Sezione di Napoli, Via P. Castellino, 111, 80131 Naples, Italy.
B. Dale
Affiliation:
Centro Fecondazione Assistita (CFA-Italia), Via Manzoni 15, 80123 Naples, Italy.
*
All correspondence to: G. Coppola. Centro Fecondazione Assistita (CFA-Italia), Via Manzoni 15, 80123 Naples, Italy. Tel: +39 081641689. Fax: +39 0815479251. e-mail: gcoppola@cfa-italia.com
All correspondence to: G. Coppola. Centro Fecondazione Assistita (CFA-Italia), Via Manzoni 15, 80123 Naples, Italy. Tel: +39 081641689. Fax: +39 0815479251. e-mail: gcoppola@cfa-italia.com
Get access Rights & Permissions [Opens in a new window]

Summary

The morphology of the sperm head has often been correlated with the outcome of in vitro fertilization, and has been shown to be the sole parameter in semen of value in predicting the success of intracytoplasmic sperm injection and intracytoplasmic morphologically selected sperm injection.

In this paper, we have studied whether digital holographic microscopy (DHM) may be useful to obtain quantitative data on human sperm head structure and compared this technique with high-power digitally enhanced Nomarski optics. The main advantage of digital holography is that high-resolution three-dimensional quantitative sample imaging may be automatically produced by numerical refocusing of a two-dimensional image at different object planes without any mechanical scanning. We show that DHM generates useful information on the dimensions and structure of human sperm, not revealed by conventional phase-contrast microscopy, in particular the volume of vacuoles, and suggest its use as an additional prognostic tool in assisted reproduction technology.

Keywords

Digitally enhanced Nomarski microscopyDigital holographic microscopyHuman sperm structureMale infertilityVacuoles
Type
Research Article
Information
Zygote , Volume 22 , Issue 4 , November 2014 , pp. 446 - 454
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Akaki, M., Nagayasu, E., Nakano, Y. & Aikawa, M. (2002). Surface charge of Plasmodium falciparum merozoites as revealed by atomic force microscopy with surface potential spectroscopy. Parasitol. Res. 88, 16–20.CrossRefGoogle ScholarPubMed
Bane, A. & Nicander, L. (1965). Pouch formations by invaginations of the nuclear envelope of bovine and porcine sperm as e sign of disturbed spermiogenesis. Nord Vet. Med. 17, 628–32.Google Scholar
Barth, A.D. & Oko, R.J. (1989). Abnormal Morphology of Bovine Spermatozoa, Ames, Iowa, USA: Iowa State University Press.Google Scholar
Bartoov, B., Eltes, F., Pansky, M., Lederman, H., Caspi, E. & Soffer, Y. (1999). Estimating fertility potential via semen analysis data. Hum. Reprod. 8, 65–70.CrossRefGoogle Scholar
Bartoov, B., Berkovitz, A., Eltes, F., Kogosowski, A., Menezo, Y. & Barak, Y. (2002). Real-time fine morphology of motile human sperm cells is associated with IVF–ICSI outcome. J. Androl. 23, 1–8.CrossRefGoogle ScholarPubMed
Berkovitz, A., Eltes, F., Soffer, Y., Zabludovsky, N., Beyth, Y., Farhi, J., Levran, D. & Bartoov, B. (1999). ART success and in vivo sperm cell selection depend on the ultramorphological status of spermatozoa. Andrologia 31, 1–8.CrossRefGoogle ScholarPubMed
Berkovitz, A., Eltes, F., Lederman, H., Peer, S., Ellenbogen, A., Feldberg, B. & Bartoov, B. (2006). How to improve IVF-ICSI outcome by sperm selection. Reprod. Biomed. Online 12, 634–8.CrossRefGoogle ScholarPubMed
Blom, E. (1977). Sperm morphology with reference to bull infertility. In Proc. First All-India Symp. Anim. Reprod., Ludhiana (ed. Blom, E.) pp. 61–81.Google Scholar
Bon, P., Maucort, G., Wattellier, B. & Monneret, S. (2009). Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells. Opt. Express 17, 13080–94.CrossRefGoogle ScholarPubMed
Carl, D., Kemper, B., Wernicke, G. & von Bally, G. (2004). Parameter optimized digital holographic microscope for high-resolution living cell analysis. Appl. Opt. 43, 6536–44.CrossRefGoogle ScholarPubMed
Charrière, F., Marian, A., Montfort, F., Kuehn, J., Colomb, T., Cuche, E., Marquet, P. & Depeursinge, C. (2006). Cell refractive index tomography by digital holographic microscopy. Opt. Lett. 31, 178–80.CrossRefGoogle ScholarPubMed
Coppola, G., Ferraro, P., Iodice, M., De Nicola, S., Finizio, A. & Grilli, S. (2004). Digital holographic microscope for static and dynamic characterization of MEMS. Meas. Sci. Technol. 1, 529–39.CrossRefGoogle Scholar
Crha, I., Zakova, J., Huser, M., Ventruba, P., Lousova, E. & Pohanka, M. (2011). Digital holographic microscopy in human sperm imaging. J. Assist. Reprod. Genet. 28, 725–9.CrossRefGoogle ScholarPubMed
Cuche, E., Marquet, P. & Depeursinge, C. (1999). Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. Appl. Opt. 38, 6994–7001.CrossRefGoogle ScholarPubMed
Dadoune, J.P. (2003). Expression of mammalian spermatozoal nucleoproteins. Microsc. Res. Tech. 61, 56–75.CrossRefGoogle ScholarPubMed
Di Caprio, G., Gioffrè, M.A., Saffioti, N.A., Grilli, S.A., Ferraro, P.A., Puglisi, R.A., Balduzzi, D.A., Galli, A.A. & Coppola, G. (2010). Quantitative label-free animal sperm imaging by means of digital holographic microscopy. IEEE J. Sel. Top. Quant. Electron. 16, 833–40.CrossRefGoogle Scholar
Dubois, F., Joannes, L. & Legros, J.C. (1999). Improved three-dimensional imaging with a digital holographic microscope with a source of partial spatial coherence. Appl. Opt. 38, 7085–94.CrossRefGoogle ScholarPubMed
Elder, K. & Dale, B. (2011). In-vitro Fertilization, 3rd edn, Cambridge, UK: Cambridge University Press, 35 pp.Google Scholar
Ferraro, P., De Nicola, S., Finizio, A., Coppola, G., Grilli, S., Magro, C. & Pierattini, G. (2003). Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging. Appl. Opt. 42, 1938–46.CrossRefGoogle ScholarPubMed
Ferraro, P., Coppola, G., Alfieri, D., De Nicola, S., Finizio, A. & Pierattini, G. (2004). Controlling images parameters in the reconstruction process of digital holograms. IEEE JSTQE 10, 829–83.Google Scholar
Ferraro, P., Grilli, S., Alfieri, D., De Nicola, S., Finizio, A., Pierattini, G., Javidi, B., Coppola, G. & Striano, V. (2005). Extended focused image in microscopy by digital holography. Opt. Express 13, 6738–49.CrossRefGoogle ScholarPubMed
Franco, J.G. Jr., Baruffi, R.L., Mauri, A.L., Petersen, C.G., Oliveira, J.B. & Vagnini, L. (2008). Significance of large nuclear vacuoles in human spermatozoa: implications for ICSI. Reprod. Biomed. Online 17, 42–5.CrossRefGoogle ScholarPubMed
Franco, J.G. Jr., Mauri, A.L., Petersen, C.G., Massaro, F.C., Silva, L.F., Felipe, V., Cavagna, M., Pontes, A., Baruffi, R.L., Oliveira, J.B. & Vagnini, L.D. (2012). Large nuclear vacuoles are indicative of abnormal chromatin packaging in human spermatozoa. Int. J. Androl. 35, 46–51.CrossRefGoogle ScholarPubMed
Gerton, G. (2002). Function of the sperm acrosome. In Fertilization (ed. Hardy, D.), pp. 265–302, San Diego: Academic Press.CrossRefGoogle Scholar
Johnson, L.A. & Truitt-Gibert, A.J. (1982). Incidence and ultrastructure of abnormalities in porcine spermatozoa. Ann. N.Y. Acad. Sci. 383, 466–8.CrossRefGoogle Scholar
Johnson, L.A. & Hurtgen, J.P. (1985). The morphological and ultrastructural appearance of the crater defect in stallion spermatozoa. Gamete Res. 12, 41–6.CrossRefGoogle Scholar
Kemper, B.D., Carl Schnekenburger, J., Bredebusch, I., Schäfer, M., Domschke, W. & von Bally, G. (2006). Investigation on living pancreas tumor cells by digital holographic microscopy. J. Biomed. Opt. 11, 34005.CrossRefGoogle ScholarPubMed
Kou, S.S., Waller, L., Barbastathis, G. & Sheppard, C.J.R. (2010). Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging. Opt. Lett. 35, 447–9.CrossRefGoogle ScholarPubMed
Kruger, T.F., Acosta, A.A., Simmons, K.F., Swanson, R.J., Matta, J.F. & Oehninger, S. (1988). Predictive value of abnormal sperm morphology in vitro fertilization. Fertil. Steril. 49, 112–7.CrossRefGoogle ScholarPubMed
Marquet, P., Rappaz, B., Magistretti, P.J., Cuche, E., Emery, Y., Colomb, T. & Depeursinge, C. (2005). Digital holographic microscopy: a non invasive contrast imaging technique allowing quantitative visualization of living cells with sub wavelength accuracy. Opt. Lett. 30, 468–70.CrossRefGoogle Scholar
Mashiach, R., Fisch, B., Eltes, F., Tadir, Y., Ovadia, J. & Bartoov, B. (1992). The relationship between sperm ultrastructural features and fertilizing capacity in vitro. Fertil. Steril. 57, 1052–7.CrossRefGoogle ScholarPubMed
Miller, D.M., Hrudka, F., Cates, W.F. & Mapletoft, R.J. (1982). Infertility in a bull with a nuclear sperm defect: a case report. Theriogenology 17, 611–21.CrossRefGoogle Scholar
Oko, R.J. (1995). Developmental expression and possible role of perinuclear theca proteins in mammalian spermatozoa. Reprod. Fertil. Dev. 7, 777–97.CrossRefGoogle ScholarPubMed
Palermo, G., Joris, H., Devroey, P. & Van Steirteghem, A.C. (1992). Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 340, 17–8.CrossRefGoogle ScholarPubMed
Rothery, AM., Gorelik, J., Bruckbauer, A., Yu, W., Korchev, Y.E. & Klenerman, D. (2003). A novel light source for SICM–SNOM of living cells. J. Microsc. 209, 94–101.CrossRefGoogle ScholarPubMed
Steptoe, P.C. & Edwards, R.G. (1978). Birth after the reimplantation of a human embryo. Lancet 12, 366.CrossRefGoogle Scholar
Wilding, M., Coppola, G., Di Matteo, L., Palagiano, A., Fusco, E. & Dale, B. (2011). Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa. J. Assist. Reprod. Genet. 28, 253–62.CrossRefGoogle ScholarPubMed
Yoshinaga, K. & Toshimori, K. (2003). Organization and modifications of sperm acrosomal molecules during spermatogenesis and epididymal maturation. Microsc. Res. Tech. 61, 39–45.CrossRefGoogle ScholarPubMed