Skip to main content Accessibility help
×
Home

Developmental potential of in vitro or in vivo matured oocytes

  • Diego D. Alcoba (a1), Anita M. Pimentel (a2), Ilma S. Brum (a3) and Helena E. Corleta (a4)

Summary

This study compared the embryological features of mature and immature oocytes (different stages) collected from stimulated cycles of in vitro fertilization (IVF). Immature oocytes were identified, classified as PI (prophase I – germinal vesicle, GV) or MI (metaphase I), were matured in vitro and fertilized using the intra-cytoplasmic sperm injection (ICSI) technique. Fertilization potential, cleavage, and subsequent transfer/cryopreservation of the embryos derived from these in vitro matured oocytes were compared with those of in vivo matured oocytes (collected at the MII stage). The characteristics of embryos derived from gametes recovered in the MI and MII stages were similar. The fertilization rate of immature oocytes recovered in PI was significantly lower than that of MII oocytes (P = 0.031), and the cleavage rate of the PI group was also lower than that of the MI (P = 0.004) and MII (P < 0.001) groups. In vitro maturation of MI oocytes is a suitable alternative when immature oocytes are recovered, as their characteristics and development are similar to those of in vivo matured oocytes. Optimization of outcomes for PI oocytes will require development of techniques that can distinguish which of these gametes will mature and fertilize.

Copyright

Corresponding author

All correspondence to: Diego Duarte Alcoba. Laboratório de Biologia Molecular Endócrina e Tumoral, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, Instituto de Ciências Básicas da Saúde, 500 Porto Alegre, Rio Grande do Sul, Brazil. Tel: +55 51 33083453. Fax: +55 51 33083656. e-mail: alcobadiego@hotmail.com

References

Hide All
Adona, P., Pires, P., Quetglas, M., Schwarz, K. & Leal, C. (2008). Prematuration of bovine oocytes with butyrolactone I: Effects on meiosis progression, cytoskeleton, organelle distribution and embryo development. Anim. Reprod. Sci. 108, 4965.
Barnes, F.L., Kausche, A., Tiglias, J., Wood, C., Wilton, L. & Trounson, A. (1996). Production of embryos from in vitro-matured primary human oocytes. Fertil. Steril. 65, 1151–6.
Ben-Ami, I., Komsky, A., Bern, O., Kasterstein, E., Komarovsky, D. & Ron-El, R. (2011). In vitro maturation of human germinal vesicle-stage oocytes: role of epidermal growth factor-like growth factors in the culture medium. Hum. Reprod. 26, 7681
Bodri, D., Vernaeve, V., Guillén, J.J., Vidal, R., Figueras, F. & Coll, O. (2006). Comparison between a GnRH antagonist and a GnRH agonist flare-up protocol in oocyte donors: a randomized clinical trial. Hum. Reprod. 21, 2246–51.
Cha, K.Y. & Chian, R.C. (1998). Maturation in vitro of immature human oocytes for clinical use. Hum. Reprod. Update 4, 103–20.
De Vos, A., Van de Velde, H., Joris, H. & Van Steirteghem, A. (1999). In-vitro matured metaphase-I oocytes have a lower fertilization rate but similar embryo quality as mature metaphase-II oocytes after intracytoplasmic sperm injection. Hum. Reprod. 14, 1859–63.
Dekel, N. (1995). Molecular control of meiosis. Trends Endocrinol. Metab. 6, 165–9.
Edirisinghe, W., Junk, S., Matson, L. & Yovich, J. (1997). Birth from cryopreserved embryos following in-vitro maturation of oocytes and intracytoplasmic sperm injection. Hum. Reprod. 12, 1056–8.
Edwards, R.G. (1965). Maturation in vitro of human ovarian oocytes. Lancet 2, 926–9.
Huang, J.Y., Chian, R.C., Gilbert, L., Fleiszer, D., Holzer, H., Dermitas, E., Elizur, S.E., Gidoni, Y., Levin, D., Son, W.Y. & Tan, S.L. (2010). Retrieval of immature oocytes from unstimulated ovaries followed by in vitro maturation and vitrification: A novel strategy of fertility preservation for breast cancer patients. Am. J. Surg. 200, 177–83.
Jaroudi, K., Hollanders, J., Sieck, U., Roca, G., El Nour, A. & Coskun, S. (1997). Pregnancy after transfer of embryos which were generated from in-vitro matured oocytes. Hum. Reprod. 12, 857–9.
Kim, B.K., Lee, S.C., Kim, K.J., Han, C.H. & Kim, J.H. (2000). In vitro maturation, fertilization, and development of human germinal vesicle oocytes collected from stimulated cycles. Fertil. Steril. 74, 1153–8.
Le Du, A., Kadoch, I., Bourcigaux, N., Doumerc, S., Bourrier, M., Chevalier, N., Fanchin, R., Chian, R., Tachdjian, G., Frydman, R. & Frydman, N. (2005). In vitro oocyte maturation for the treatment of infertility associated with polycystic ovarian syndrome: the French experience. Hum. Reprod. 20, 420–4.
Lee, J.E., Kim, S.D., Jee, B.C., Suh, C.S. & Kim, S.H. (2011). Oocyte maturity in repeated ovarian stimulation. Clin. Exp. Reprod. Med. 38, 234–7.
Liu, J., Katz, E., Garcia, J., Compton, G. & Baramki, T. (1997). Successful in vitro maturation of human oocytes not exposed to human chorionic gonadotropin during ovulation induction, resulting in pregnancy. Fertil. Steril. 67, 566–8.
Liu, J., Lu, G., Qian, Y., Mao, Y. & Ding, W. (2003). Pregnancies and births achieved from in vitro matured oocytes retrieved from poor responders undergoing stimulation in in vitro fertilization cycles. Fertil. Steril. 80, 447–9.
McAvey, B., Zapantis, A., Jindal, S.K., Lieman, H.J. & Polotsky, A.J. (2011). How many eggs are needed to produce an assisted reproductive technology baby: is more always better? Fertil. Steril. 96, 332–5.
Nagy, Z.P., Cecile, J., Liu, J., Loccufier, A., Devroey, P. & Van Steirteghem, A. (1996). Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: case report. Fertil. Steril. 65, 1047–50.
Oktay, K., Demirtas, E., Son, W.Y., Lostritto, K., Chian, R.C. & Tan, S.L. (2008). In vitro maturation of germinal vesicle oocytes recovered after premature luteinizing hormone surge: description of a novel approach to fertility preservation. Fertil. Steril. 89, 228.e219–22.
Russell, J.B. (1999). Immature oocyte retrieval with in-vitro oocyte maturation. Curr. Opin. Obstet. Gynecol. 11, 289–96.
Sathananthan, A., Selvaraj, K. & Trounson, A. (2000). Fine structure of human oogonia in the foetal ovary. Mol. Cell. Endocrinol. 161, 38.
Smith, S.D., Mikkelsen, A. & Lindenberg, S. (2000). Development of human oocytes matured in vitro for 28 or 36 hours. Fertil. Steril. 73, 541–4.
Son, W.Y., Lee, S.Y. & Lim, J.H. (2005). Fertilization, cleavage and blastocyst development according to the maturation timing of oocytes in in vitro maturation cycles. Hum. Reprod. 20, 3204–7.
Son, W.Y., Chung, J.T., Demirtas, E., Holzer, H., Sylvestre, C., Buckett, W., Chian, R.C. & Tan, S.L. (2008). Comparison of in-vitro maturation cycles with and without in-vivo matured oocytes retrieved. Reprod. Biomed. Online 17, 5967.
Suikkari, A.M. & Söderström-Anttila, V. (2007). In-vitro maturation of eggs: is it really useful? Best Pract. Res. Clin. Obstet. Gynaecol. 21, 145–55.
Söderström-Anttila, V., Salokorpi, T., Pihlaja, M., Serenius-Sirve, S. & Suikkari, A.M. (2006). Obstetric and perinatal outcome and preliminary results of development of children born after in vitro maturation of oocytes. Hum. Reprod. 21, 1508–13.
Trounson, A., Wood, C. & Kausche, A. (1994). In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil. Steril. 62, 353–62.
Trounson, A., Anderiesz, C. & Jones, G. (2001). Maturation of human oocytes in vitro and their developmental competence. Reproduction 121, 5175.
Tucker, M., Wright, G., Morton, P. & Massey, J. (1998). Birth after cryopreservation of immature oocytes with subsequent in vitro maturation. Fertil. Steril. 70, 578–9.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed