Skip to main content Accessibility help
×
Home

Developmental competence and expression of the MATER and ZAR1 genes in immature bovine oocytes selected by brilliant cresyl blue

  • Gustavo Bruno Mota (a1), Ribrio Ivan Tavares Pereira Batista (a2), Raquel Varella Serapião (a2), Mariana Cortes Boité (a2), João Henrique Moreira Viana (a2), Ciro Alexandre Alves Torres (a1) and Luiz Sergio de Almeida Camargo (a3)...

Summary

The objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ groups. The relative expression of MATER and ZAR1 genes was evaluated by real-time PCR in immature oocytes collected from the control, holding control, BCB+ and BCB groups. Despite the relative expression of MATER in holding control, BCB+ and BCB were down regulated in comparison to control group there was no statistical difference (p > 0.05) in the relative expression of MATER and ZAR1 transcripts among groups. The results indicate that the BCB dye detects immature oocyte populations with different developmental competence, although no improvement in in vitro embryo production using oocytes exposed or not to BCB was observed. Development competence of immature oocytes exposed to BCB does not seem to be associated with variations in the expression of MATER and ZAR1 transcripts.

Copyright

Corresponding author

All correspondence to: Luiz S. A. Camargo. Embrapa Gado de Leite, Juiz de Fora, MG, 36038–330, Brazil. Tel: +55 32 32494800. Fax: +55 32 32494701. e-mail: camargo@cnpgl.embrapa.br

References

Hide All
Alm, H., Torner, H., Löhrke, B., Viergutz, T., Ghoneim, I.M. & Kanitz, W. (2005). Bovine blastocyst development rate in vitro is influenced by selection of oocytes by brilliant cresyl blue staining before IVM as indicator for glucose-6-phosphate dehydrogenase activity. Theriogenology 63, 2194–205.
Bhojwani, S., Alm, H., Torner, H., Kanitz, W. & Poehland, R. (2007). Selection of developmentally competent oocytes through brilliant cresyl blue stain enhances blastocyst development rate after bovine nuclear. Theriogenology 67, 341–5.
Camargo, L.S.A., Viana, J.H.M., Ramos, A.A., Serapião, R.V., de Sa, W.F., Ferreira, A.M., Guimarães, M.F.M. & do Vale Filho, V.R. (2007). Developmental competence and expression of the Hsp 70.1 gene in oocytes obtained from Bos indicus and Bos taurus dairy cows in a tropical environment. Theriogenology 68, 626–32.
Conover, J.C., Temeles, G.L., Zimmermann, J.W., Burke, B. & Schultz, R.M. (1991). Stage-specific expression of a family of proteins that are major products of zygotic gene activation in the mouse embryo. Dev. Biol. 144, 392404.
Crozet, N., Kanka, J., Motlik, J. & Fulka, J. (1986). Nucleolar fine structure and RNA synthesis in bovine oocytes from antral follicles. Gamete Res. 14, 6573.
Dean, J. (2002). Oocyte-specific genes regulate follicle formation, fertility and early mouse development. J. Reprod. Immunol. 53, 171–80.
El Shourbagy, S.H., Spikings, E.C., Freitas, M. & St John, J.C. (2006). Mitochondria directly influence fertilization outcome in pig. Reproduction 131, 233–45.
Ericsson, S.A., Boice, M.L., Funahashi, H. & Day, B.N. (1993). Assessment of porcine oocytes using brilliant cresyl blue (abstract). Theriogenology 39, 214.
Fair, T. & Hyttel, P. (1999). Nucleolar proteins in bovine oocytes (abstract). Theriogenology 51, 371.
Fair, T., Hyttel, P. & Greve, T. (1995). Bovine oocyte size in relationship to follicular diameter, maturational competence and rna synthesis (abstract). Theriogenology 43, 209.
Gandolfi, F. (1996). Intra-ovarian regulation of oocyte development competence in cattle. Zygote 4, 323–6.
Gandolfi, T.A.L.B. & Gandolfi, F. (2001). The maternal legacy to the embryo: cytoplasmic components and their effects on early development. Theriogenology 55, 1255–76.
Ghanem, N., Hölker, M., Rings, F., Jennen, D., Tholen, E., Sirard, M.A., Torner, H., Kanitz, W., Schellander, K. & Tesfaye, D. (2007). Alterations in transcript abundance of bovine oocytes recovered at growth and dominance phases of the first follicular wave. BMC Dev. Biol. 7, 90.
Gordon, I. (1994). Laboratory Production of Cattle Embryo. London: CAB International. Cambridge University Press.
Hyttel, P., Fair, T., Callesen, H. & Greve, T. (1997). Oocyte growth, capacitation and final maturation in cattle. Theriogenology 47, 2332.
Ishizaki, C., Watanabe, H., Bhuiyan, M.M.U. & Fukui, Y. (2009). Developmental competence of porcine oocytes selected by brilliant cresyl blue and matured individually in a chemically defined culture medium. Theriogenology 72, 7280.
Kątska-Książkiewicz, L., Opiela, J. & Ryńska, B. (2007). Effects of oocyte quality, semen donor and embryo co-culture system on the efficiency of blastocyst production in goats. Theriogenology 68, 736–44.
Liu, Z. & Foote, R.H. (1997). Effects of amino acids and alpha-amanitin on bovine embryo development in a simple protein-free medium. Mol. Reprod. Dev. 46, 278–85.
Lonergan, P., Monaghan, P., Rizos, D., Boland, M.P. & Gordon, I. (1994). Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization and culture in vitro. Mol. Reprod. Dev. 37, 4853.
Machatkova, M., Krausova, K., Jokesova, E. & Tomanek, M. (2004). Developmental competence of bovine oocytes: effects of follicle size and the phase of follicular wave on in vitro embryo production. Theriogenology 61, 329–35.
Mangia, F. & Epstein, C.J. (1975). Biochemical studies of growing mouse oocytes: preparation of oocytes and analysis of glucose-6-phosphate dehydrogenase and lactate dehydrogenase activities. Dev. Biol. 45, 211–20.
Manjunatha, B.M., Gupta, P.S.P., Devaraj, M., Ravindra, J.P. & Nandi, S. (2007). Selection of developmentally competent buffalo oocytes by brilliant cresyl blue staining before IVM. Theriogenology 68, 1299–304.
Minami, N., Suzuki, T. & Tsukamoto, S. (2007). Zygotic gene activation and maternal factors in mammals. J. Reprod. Dev. 53, 707–15.
Opiela, J., Kątska-Książkiewicz, L., Lipiński, D., Słomski, R., Bzowska, M. & Ryńska, B. (2008). Interactions among activity of glucose-6-phosphate dehydrogenase in immature oocytes, expression of apoptosis-related genes Bcl-2 and Bax and developmental competence following IVP in cattle. Theriogenology 69, 546–55.
Pennetier, S., Perreau, C., Uzbekova, S., Thélie, A., Delaleu, B., Mermillod, P. & Dalbiès-Tran, R. (2006). MATER protein expression and intracellular localization throughout folliculogenesis and preimplantation embryo development in the bovine. BMC Dev. Biol. 6, 26.
Pennetier, S., Uzbekova, S., Perreau, C., Papillier, P., Mermillod, P. & Tran, P.R. (2004). Spatio-temporal expression of the germ cell marker genes MATER, ZAR1, GDF9, BMP15 and VASA in adult bovine tissues, oocytes and preimplantation embryos. Biol. Repr. 71, 1359–66.
Pujol, M., López-Béjar, M. & Paramio, M.T. (2004). Developmental competence of heifer oocytes selected using the brilliant cresyl blue (BCB) test. Theriogenology 61, 735–44.
Ramakers, C., Ruijter, J.M., Deprez, R.H. & Moorman, A.F.M. (2003). Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci. Lett. 339, 62–6.
Roca, J., Martinez, E., Vazquez, J.M. & Lucas, X. (1998). Selection of immature pig oocytes for homologous in vitro penetration assays with the brilliant cresyl blue test. Reprod. Fert. Dev. 10, 479–85.
Rodríguez-González, E., López-Béjar, M., Velilla, E. & Paramio, M.T. (2002). Selection of prepubertal goat oocytes using the brilliant cresyl blue test. Theriogenology 57, 1397–409.
Schultz, R.M. (2002). The molecular foundations of the maternal to zygotic transition in the preimplantation embryo. Hum. Reprod. Up. 8, 323–31.
Seshagiri, P.B., McKenzie, D.I., Bavister, B.D., Williamson, J.L. & Aiken, J.M. (1992). Golden hamster embryonic genome activation occurs at the two-cell stage: correlation with major developmental changes. Mol. Reprod. Dev. 32, 229–35.
Sirard, M.A., Richard, F., Blondin, P. & Robert, C. (2006). Contribution of the oocyte to embryo quality. Theriogenology 65, 126–36.
Thélie, A., Papillier, P., Pennetier, S., Perreau, C., Traverso, J.M., Uzbekova, S., Mermillod, P., Joly, C., Humblot, P. & Dalbiès-Tran, R. (2007). Differential regulation of abundance and deadenylation of maternal transcripts during bovine oocyte maturation in vitro and in vivo. BMC Dev. Biol. 7, 125.
Tian, W.N., Braunstein, L.D., Pang, J., Stuhlmeier, K.M., Xi, Q.C., Tian, X. & Stanton, R.C. (1998). Importance of glucose-6-phosphate dehydrogenase activity for cell growth. J. Biol. Chem. 273, 10609–17.
Tong, Z-B., Gold, L., Pfeifer, K.E., Dorward, H., Lee, E., Bondy, C.A., Dean, J. & Nelson, L.M. (2000). Mater, a maternal effect gene required for early embryonic development in mice. Nat. Genet. 26, 267–8.
Torner, H., Ghanem, N., Ambros, C., Hölker, M., Tomek, W., Phatsara, C., Alm, H., Sirard, M.A., Kanitz, W., Schellander, K. & Tesfaye, D. (2008). Molecular and subcellular characterisation of oocytes screened for their developmental competence based on glucose-6-phosphate dehydrogenase activity. Reproduction 135, 197212.
Tsutsumi, O., Satoh, K., Taketani, Y. & Kato, T. (1992). Determination of enzyme activities of energy metabolism in the maturing rat oocyte. Mol. Reprod. Dev. 33, 333–7.
Urdaneta, A., Jiménez-Macedo, A.R., Izquierdo, D. & Paramio, M.T. (2003). Supplementation with cysteamine during maturation and embryo culture on embryo development of prepubertal goat oocytes selected by the brilliant cresyl blue test. Zygote 11, 347–54.
Urner, F. & Sakkas, D. (2005). Involvement of the pentose phosphate pathway and redox regulation in fertilization in the mouse. Mol. Reprod. Dev. 70, 494503.
Uzbekova, S., Roy-Sabau, M., Dalbiès-Tran, R., Perreau, C., Papillier, P., Mompart, F., Thelie, A., Pennetier, S., Cognie, J., Cadoret, V., Royere, D., Monget, P. & Mermillod, P. (2006). Zygote arrest 1 gene in pig, cattle and human: evidence of different transcript variants in male and female germ cells. Reprod. Biol. Endocrinol. 4, 12.
Viana, J.H.M. & Camargo, L.S.A. (2007). Bovine embryo production in Brazil: a new scenario. Acta Sci. Vet. 35, 920–4.
Viana, J.H.M., Camargo, L.S.A., Ferreira, A.M., de Sa, W.F., Fernandes, C.A.C. & Marques Junior, A.P. (2004). Short intervals between ultrasonographically guided follicle aspiration improve oocyte quality but do not prevent establishment of dominant follicles in the Gir breed (Bos indicus) of cattle. Anim. Reprod. Sci. 84, 112.
Wongsrikeao, P., Otoi, T., Yamasaki, H., Agung, B., Taniguchi, M., Naoi, H., Shimizu, R. & Nagai, T. (2006). Effects of single and double exposure to brilliant cresyl blue on the selection of porcine oocytes for in vitro production of embryos. Theriogenology 66, 366–72.
Wood, J.R., Dumesic, D.A., Abbott, D.H. & Strauss, J.F. (2007). Molecular abnormalities in oocytes from women with polycystic ovary syndrome revealed by microarray analysis. J. Clin. Endocrinol. Metab. 92, 705–13.
Wu, Y-G., Liu, Y., Zhou, P., Lan, G-C., Han, D., Miao, D-Q. & Tan, J-H. (2007). Selection of oocytes for in vitro maturation by brilliant cresyl blue staining: a study using the mouse model. Cell Res. 17, 722–31.
Wu, X., Viveiros, M.M., Eppig, J.J., Bai, Y., Fitzpatrick, S.L. & Matzuk, M.M. (2003). Zygote arrest 1 (Zar1) is a novel maternal-effect gene critical for the oocyte-to-embryo transition. Nat. Genet. 33, 187–91.

Keywords

Developmental competence and expression of the MATER and ZAR1 genes in immature bovine oocytes selected by brilliant cresyl blue

  • Gustavo Bruno Mota (a1), Ribrio Ivan Tavares Pereira Batista (a2), Raquel Varella Serapião (a2), Mariana Cortes Boité (a2), João Henrique Moreira Viana (a2), Ciro Alexandre Alves Torres (a1) and Luiz Sergio de Almeida Camargo (a3)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed