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Determination of new types of DNA lesions in human sperm

Published online by Cambridge University Press:  01 February 2008

C. Badouard ,
Y. Ménézo ,
G. Panteix ,
J.L. Ravanat ,
T. Douki ,
J. Cadet  and
A. Favier
C. Badouard
Affiliation:
Laboratoire des Lésions des Acide Nucléiques LCIB UMR-E 3 CEA-UJF DRFMC, CEA Grenoble, 17 rue des martyrs, 38054 Grenoble Cedex 9.France.
Y. Ménézo*
Affiliation:
Laboratoire d'Eylau, 55 rue St Didier, 75116 ParisFrance.
G. Panteix
Affiliation:
Laboratoire d'Eylau, 55 rue St Didier, 75116 ParisFrance.
J.L. Ravanat
Affiliation:
Laboratoire Marcel Merieux. Avenue Tony Garnier, 69007 Lyon, France.
T. Douki
Affiliation:
Laboratoire des Lésions des Acide Nucléiques LCIB UMR-E 3 CEA-UJF DRFMC, CEA Grenoble, 17 rue des martyrs, 38054 Grenoble Cedex 9.France.
J. Cadet
Affiliation:
Laboratoire des Lésions des Acide Nucléiques LCIB UMR-E 3 CEA-UJF DRFMC, CEA Grenoble, 17 rue des martyrs, 38054 Grenoble Cedex 9.France.
A. Favier
Affiliation:
Laboratoire des Lésions des Acide Nucléiques LCIB UMR-E 3 CEA-UJF DRFMC, CEA Grenoble, 17 rue des martyrs, 38054 Grenoble Cedex 9.France.
*
1All correspondence to: Yves Ménézo, Laboratoire d'Eylau, 55 rue St Didier, 75116 ParisFrance. Tel: +33 607419368. Fax: +33 153706494. e-mail: yves.menezo@club-internet.fr
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Summary

Careful attention has been focused recently on DNA quality in human IVF. Therefore a variety of methods has been developed to evaluate DNA integrity, especially concerning fragmentation. Using liquid chromatography and mass spectrometry (LC/MS/MS) for our best sperm samples, we have established reference values for several oxidative lesions, in order to gain insights into the cause of DNA lesions. Besides 8-oxodeoxyguanosine, we found rather high levels of two ethenonucleosides: 1,N6-ethenoadenosine and 1,N2-ethenoguanosine. These compounds probably arise from a reaction with 4-hydroxy-2-nonenal, the main aldehyde compound released during lipid peroxidation, or after occupational exposure to vinyl chloride. The quantity of chlorinated bases detected is low. All of this decay has to be repaired by the oocytes at the time of fertilization or immediately after. This aspect should not be overlooked in assisted reproductive technology, in order to understand risks and limitations.

Keywords

DNA lesionsHPLC – MS/MSLipid peroxidationOxidative stressSperm
Type
Research Article
Information
Zygote , Volume 16 , Issue 1 , February 2008 , pp. 9 - 13
Copyright
Copyright © Cambridge University Press 2008

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