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Cryopreservation of collared peccary (Pecari tajacu L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP®-116c)

Published online by Cambridge University Press:  17 September 2018

José A. B. Bezerra ,
Andréia M. Silva ,
Patrícia C Sousa ,
Lívia B. Campos ,
Érica C. G. Praxedes ,
Luana G. P. Bezerra ,
Thibério S. Castelo ,
Ana L. P. Souza  and
Alexandre R. Silva Open the ORCID record for Alexandre R. Silva [Opens in a new window]
José A. B. Bezerra
Affiliation:
Laboratory of Animal Germplasm Conservation (LCGA), Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil
Andréia M. Silva
Affiliation:
Laboratory of Animal Germplasm Conservation (LCGA), Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil
Patrícia C Sousa
Affiliation:
Laboratory of Animal Germplasm Conservation (LCGA), Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil
Lívia B. Campos
Affiliation:
Laboratory of Animal Germplasm Conservation (LCGA), Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil
Érica C. G. Praxedes
Affiliation:
Laboratory of Animal Germplasm Conservation (LCGA), Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil
Luana G. P. Bezerra
Affiliation:
Laboratory of Animal Germplasm Conservation (LCGA), Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil
Thibério S. Castelo
Affiliation:
Laboratory of Animal Germplasm Conservation (LCGA), Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil
Ana L. P. Souza
Affiliation:
Laboratory of Animal Germplasm Conservation (LCGA), Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil
Alexandre R. Silva*
Affiliation:
Laboratory of Animal Germplasm Conservation (LCGA), Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil
*
Author for correspondence: Alexandre R. Silva. Laboratory of Animal Germplasm Conservation – LCGA, Department of Animal Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, Rio Grande do Norte, Brazil. E-mail: legio2000@yahoo.com
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Summary

The aim of this study was to establish a functional freezing–thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed. In addition, to evaluate the survival of frozen–thawed sperm, a thermal resistance test (TRT) was executed. Samples preserved using Tris were in better condition compared with those preserved using ACP®, showing higher values for most assessments performed, including CASA and the TRT (P<0.05). After determining Tris to be the better of the two extenders, additional samples were thawed using different thawing rates (37°C/60 s, 55°C/7 s, 70°C/8 s). Sperm thawed at 37°C/60 s had the greatest preservation (P<0.05) of viability (54.1 ± 5.9%) and functional membrane integrity (43.2 ± 5.4%), and had higher values for various CASA parameters. In conclusion, we suggest the use of a Tris-based extender added to egg yolk and glycerol for the cryopreservation of epididymal sperm obtained from collared peccaries. In order to achieve better post-thawing sperm quality, we suggest that samples should be thawed at 37°C/60 s.

Keywords

BiobankEpididymisTayassu tajacuWild pigWildlife
Type
Research Article
Information
Zygote , Volume 26 , Issue 4 , August 2018 , pp. 301 - 307
Copyright
© Cambridge University Press 2018 

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