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Crucial surviving aspects for vitrified in vitro-produced bovine embryos

  • Mateus José Sudano (a1), Daniela Martins Paschoal (a2), Tatiana da Silva Rascado (a2), Letícia Ferrari Crocomo (a2), Luis Carlos Oña Magalhães (a2), Alício Martins Junior (a3), Rui Machado (a4) and Fernanda da Cruz Landim-Alvarenga (a1)...


The objective of the present study was to correlate some parameters (cleavage, blastocyst production, quality degree score, total cell number, fresh apoptosis and lipid content) with embryo survival after cryopreservation. A total of 1727 in vitro-produced bovine blastocysts were used to establish the parameters (mean ± standard error of the mean (SEM)) for cleavage (85.6 ± 0.8), blastocyst production (39.9 ± 1.4), quality degree score (1.6 ± 0.1), total cell number (140.1 ± 2.9), fresh apoptosis (20.8 ± 1.1) and lipid content (21.3 ± 0.8 droplets). On the same way 1316 blastocysts were vitrified for the determination of post-cryopreservation embryo survival (49.4 ± 1.9). Fresh apoptosis rate and total lipid droplets value were correlated (P < 0.05) with embryo survival after cryopreservation (r = 0.91 and r = 0.59; respectively). However, cleavage, blastocyst production, quality degree score and total cell number were not correlated (P > 0.05) with embryo cryotolerance (r = 0.23, r = 0.38, r = 0.22 and r = 0.28; respectively). Therefore, the increased lipid content was moderately correlated with apoptosis in vitrified blastocysts. On the other hand, increased apoptosis in fresh blastocysts was strongly correlated with apoptosis in vitrified blastocysts, which indicated that the apoptosis rate in fresh embryos was a better parameter than the lipid content to predict post-vitrification embryo survival.


Corresponding author

All correspondence to: Fernanda da Cruz Landim-Alvarenga or Mateus José Sudano São Paulo State University – UNESP, School of Veterinary Medicine and Animal Science–FMVZ, Department of Animal Reproduction and Veterinary Radiology, Rubião Jr. s/n°, Botucatu–SP, Brazil, 18618-970. Tel:/Fax: +55 14 38116249. e-mail: or


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