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Comparison of different extenders on the recovery and longevity of epididymal sperm from Spix's yellow-toothed cavies (Galea spixii Wagler, 1831)

Published online by Cambridge University Press:  01 March 2017

Andréia Maria da Silva ,
Patrícia Cunha Sousa ,
Lívia Batista Campos ,
José Artur Brilhante Bezerra ,
Arthur Emannuel de Araújo Lago ,
Moacir Franco de Oliveira  and
Alexandre Rodrigues Silva
Andréia Maria da Silva
Affiliation:
Laboratório de Conservação e Germoplasma Animal, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, RN, Brazil
Patrícia Cunha Sousa
Affiliation:
Laboratório de Conservação e Germoplasma Animal, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, RN, Brazil
Lívia Batista Campos
Affiliation:
Laboratório de Conservação e Germoplasma Animal, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, RN, Brazil
José Artur Brilhante Bezerra
Affiliation:
Laboratório de Conservação e Germoplasma Animal, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, RN, Brazil
Arthur Emannuel de Araújo Lago
Affiliation:
Laboratório de Conservação e Germoplasma Animal, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, RN, Brazil
Moacir Franco de Oliveira
Affiliation:
Laboratório de Conservação e Germoplasma Animal, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, RN, Brazil
Alexandre Rodrigues Silva*
Affiliation:
Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido (UFERSA), BR 110, Km 47, Bairro Pres. Costa e Silva. CEP: 59.625–900 Mossoró, RN, Brazil
*
All correspondence to: A.R. Silva Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido (UFERSA), BR 110, Km 47, Bairro Pres. Costa e Silva. CEP: 59.625–900 Mossoró, RN, Brazil. Fax: +55 84 3317 8360. E-mail: alexrs2000@gmail.com
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Summary

The aim of this study was to evaluate the performance of cavy (Galea spixii) epididymal sperm following addition to TES or TRIS extenders and using a thermal resistance test (TRT), as well as fluorescence analysis as a complementary method to predict the viability of these gametes. Nine testicle–epididymis complexes were used for sperm collection using a flotation method. Epididymis tails were sliced and one was immersed in 3 ml of TRIS buffer, and the other in 3 ml of TES, for 5 min. After sperm recovery, the samples were subjected to a TRT which involved incubation in a water bath at 37°C for 3 h. During incubation, sample parameters were assessed at 0, 15, 30, 60, 90, 120, 150 or 180 min intervals. Results indicated that the TRIS diluent was more efficient than TES (P < 0.05) for the maintenance of sperm parameters in Spix's yellow-toothed cavies over the whole TRT, maintaining sperm longevity for an extended time. In conclusion, we indicate the use of TRIS diluent for recovery and maintenance of longevity of epididymal sperm from cavies (G. spixii).

Keywords

Fluorescent probeRodentsSperm analysisThermal resistanceWildlife
Type
Research Article
Information
Zygote , Volume 25 , Issue 2 , April 2017 , pp. 176 - 182
Copyright
Copyright © Cambridge University Press 2017 

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