Skip to main content Accessibility help
×
Home

Antioxidant treatment during preservation of bovine ovaries increased the development potential of embryos

  • Yoshikazu Nagao (a1) (a2) (a3), Yumiko Harada (a2), Mari Yamaguchi (a2), Akane Igarashi (a2), Yuki Ooshima (a2) and Yoku Kato (a3)...

Summary

The overnight preservation of bovine ovaries would be highly useful in the subsequent harvest of viable oocytes for reproductive study. The present study aimed to optimize conditions for overnight preservation of bovine ovaries by examining the effects of temperature, solution and supplementation. In Experiment 1, the rate of development to the blastocyst stage of oocytes derived from ovaries preserved at 15°C was higher than that at either 5 or 25°C (p < 0.05). In Experiment 2, the rate of development to the blastocyst stage of oocytes derived from ovaries preserved in University of Wisconsin solution was higher than when PBS or saline was used (p < 0.05). In Experiment 3, oocytes preserved in saline supplemented with 0.3 mM glutathione (GSH) exhibited an increase in the rate of blastocyst formation compared with oocytes supplemented with 0 or 3 mM GSH (p < 0.05). In Experiment 4, supplementation with 10 μM epigallocatechin gallate during ovary preservation increased the rate of blastocyst formation (p < 0.05). The blastocysts derived from ovaries stored in saline supplemented with GSH at 15°C for 24 h were shown to develop into normal offsprings following transfer to recipient heifers. Our studies indicate that bovine IVM/IVF embryos derived from ovaries preserved in saline supplemented with an antioxidant at 15°C for 24 h can successfully develop to the blastocyst stage and result in offspring.

Copyright

Corresponding author

All correspondence to: Yoshikazu Nagao. University Farm, Faculty of Agriculture, Utsunomiya University, Shimokomoriya 443, Mohka, Tochigi 321–4415, Japan. Tel/Fax: +81 285 84 1321. e-mail: ynagao@cc.utsunomiya-u.ac.jp

References

Hide All
Abe, S. & Shioya, Y. (1996). Effects of temperature and duration of preservation of bovine ovaries in physiological saline on the development of bovine embryos derived from follicular oocytes matured and fertilized in vitro. Anim. Sci. Technol. Jpn 67, 633–8.
Ali, A.A., Bilodeau, J.F. & Sirard, M.A. (2003). Antioxidant requirements for bovine oocytes vary during in vitro maturation, fertilization and development. Theriogenology 59, 939–49.
Blondin, P., Coenen, K., Guilbault, L.A. & Sirard, M.A. (1997). In vitro production of bovine embryos: developmental competence is acquired before maturation. Theriogenology 47, 1061–75.
Collins, G.M. & Wicomb, W.N. (1992). New organ preservation solutions. Kidney Int. Suppl. 38, S197S202.
Delcorso, C.A., Cappiello, M. & Mura, U. (1994) Thiol dependent oxidation of enzymes: the last chance against oxidative stress. Int. J. Biochem. 26, 745–50.
Flood, M.R., Gage, T.L. & Bunch, T.D. (1993). Effect of various growth-promoting factors on preimplantation bovine embryo development in vitro. Theriogenology 39, 823–33.
Guang, Z.W., Yu, S.D. & Xu, Z.R. (2007). Improvement in bovine embryo production in vitro by treatment with green tea polyphenols during in vitro maturation of oocytes. Anim. Reprod. Sci. 100, 2231.
Guerin, P., Mouatassim, S. E.I. & Menezo, Y. (2001). Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum. Reprod. Update 7, 175–89.
Guo, Q., Zhao, B.L., Li, M.F., Shen, S.R. & Xin, W.J. (1996). Studies on protective mechanisms of four components of green tea polyphenols against lipid peroxidation in synaptosomes. Biochim. Biophys. Acta 1304, 210–22.
Iwata, H., Hayashi, T., Sato, H., Kimura, K., Kuwayama, T. & Monji, Y. (2005). Modification of ovary stock solution with magnesium and raffinose improves the developmental competence of oocytes after long preservation. Zygote 13, 303–8.
Jaeschke, H., Smith, C.V. & Mitchell, J.R. (1988). Reactive oxygen species during ischemia-reflow injury in isolated perfused rat liver. J. Clin. Invest. 81, 1240–6.
Jamieson, N.V., Sundberg, R., Lindell, S., Claesson, K., Moen, J., Vreugdenhil, P.K., Wight, D.G., Southard, J.H. & Belzer, F.O. (1988). Preservation of the canine liver for 24–48 hours using simple cold storage with UW solution. Transplantation. 46, 517–22.
Koyama, I., Bulkley, G.B., Williams, G.M. & Im, M.J. (1985). The role of oxygen free radicals in mediating the reperfusion injury of cold-preserved ischemic kidneys. Transplantation 40, 590–5.
Lafleur, M.V.M., Hoorweg, J.J., Joenje, H., Westmijze, E.J. & Retel, J. (1994). The ambivalent role of glutathione in the protection of DNA against single oxygen. Free. Radic. Res. 21, 917.
Matos, D.G., Furnus, C.C., Moses, D.F. & Baldassarre, H. (1995). Effect of cysteamine on glutathione level and developmental capacity of bovine oocyte matured in vitro. Mol. Reprod. Dev. 42, 432–6.
Matsushita, S., Tani, T., Kato, Y. & Tsunoda, Y. (2004). Effect of low-temperature bovine ovary storage on the maturation rate and developmental potential of follicular oocytes after in vitro fertilization, parthenogenetic activation, or somatic cell nucleus transfer. Anim. Reprod. Sci. 84, 293301.
Nagao, Y., Saeki, K., Hoshi, M., Takahashi, Y. & Kanagawa, H. (1995). Effects of water quality on in vitro fertilization and development of bovine oocytes in protein-free medium. Theriogenology 44, 433–44.
Nagao, Y., Iijima, R. & Saeki, K. (2008). Interaction between embryos and culture conditions during in vitro development of bovine early embryos. Zygote 16, 127–33.
Roberts, R.F., Nishanian, G. P., Carey, J.N., Sakamaki, Y., Starnes, V.A. & Barr, M.L. (1999). A comparison of the new preservation solution Celsior to Euro-Collins and University of Wisconsin solutions in lung reperfusion injury. Transplantation 67, 152–5.
Saeki, K., Hoshi, M., Leibfried, M.L. & First, N.L. (1990). In vitro fertilization and development of bovine oocytes matured with commercially available follicle stimulating hormone. Theriogenology 34, 1035–9.
Schroeder, P., Klotz, L.O. & Sies, H. (2003). Amphiphilic properties of (–)epicatechin and their significance of protection of cells against peroxynitrite. Biochem Biophys Res Commun. 307, 6973.
Vinson, J.A., Dabbagh, Y., Serry, M. & Jang, J. (1995). Plant flavonoids, especially tea flavonols, are powerful antioxidants using an in vitro oxidation model for heart disease. J. Agric. Food. Chem. 43, 2800–2.
Vreugdenhil, P.K., Belzer, F.O. & Southard, J.H. (1991). Effect of cold storage on tissue and cellular glutathione. Cryobiology 28, 143–9.
Yao, N., Wan, P.C., Hao, Z.D., Gao, F.F., Yang, L., Cui, M.S., Wu, Y., Liu, J.H., Liu, S., Chen, H. & Zeng, S.M. (2009). Expression of interferon-tau mRNA in bovine embryos derived from different procedures. Reprod Domest Anim. 44, 132–9.
Yoshizawa, M., Konno, H., Zhu, S., Kageyama, S., Fukui, E., Muramatsu, S., Kim, S. & Araki, Y. (1999). Chromosomal diagnosis in each individual blastomere of 5- to 10-cell bovine embryos derived from in vitro fertilization. Theriogenology 51, 1239–50.

Keywords

Antioxidant treatment during preservation of bovine ovaries increased the development potential of embryos

  • Yoshikazu Nagao (a1) (a2) (a3), Yumiko Harada (a2), Mari Yamaguchi (a2), Akane Igarashi (a2), Yuki Ooshima (a2) and Yoku Kato (a3)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed