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Aldehyde dehydrogenase in fresh primordial germ cells as a marker of cell ‘stemness’

  • Andrea Svoradová (a1), Jaromír Vašíček (a2) (a3), Alexander Ostró (a4) and Peter Chrenek (a2) (a3) (a5)


Chicken primordial germ cells (PGCs) are the primary pluripotent stem cell types that will differentiate towards germ cells. High aldehyde dehydrogenase (ALDH) activity is considered as a functional marker for the detection of cell ‘stemness’. In our study the ALDEFLUOR™ kit was used for determination of ALDH activity in PGCs. PGCs were co-stained with diethylaminobenzaldehyde (DEAB) and ALDH and analyzed by flow cytometry. Our results showed a small cell population (8.0 ± 3.3%) upon preincubation of the cells with the specific inhibitor DEAB, however cells without inhibitor staining showed a fluorescence shift as an ALDH-positive population (70.5 ± 1.6%). These findings indicate higher expression of ALDH in PGCs and ALDH activity can therefore be used as a new functional marker for the detection of cell ‘stemness’ in chicken PGCs. These results may have importance for characterization of PGCs as a potential genetic resource in poultry. Further research is necessary to elucidate the role of this functional marker in these cells.


Corresponding author

*Address for correspondence: A. Svoradová et al. (2018) Constantine the Philosopher University in Nitra, Faculty of Natural Sciences, Department of Zoology and Anthropology, Tr. A. Hlinku 1, 949 74 Nitra, Slovak Republic. Tel: +421 37 640 8720. E-mail:


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Aldehyde dehydrogenase in fresh primordial germ cells as a marker of cell ‘stemness’

  • Andrea Svoradová (a1), Jaromír Vašíček (a2) (a3), Alexander Ostró (a4) and Peter Chrenek (a2) (a3) (a5)


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