Hostname: page-component-8448b6f56d-c4f8m Total loading time: 0 Render date: 2024-04-20T01:12:43.178Z Has data issue: false hasContentIssue false
  • English
  • Français

Administration of cyclosporin A to recipients improves the potential of mouse somatic cell nuclear-transferred oocytes to develop to fetuses

Published online by Cambridge University Press:  04 May 2011

Yuta Tsuji ,
Yoko Kato  and
Yukio Tsunoda
Yuta Tsuji
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara 631–8505, Japan.
Yoko Kato
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara 631–8505, Japan.
Yukio Tsunoda*
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, Japan
*
All correspondence to: Yukio Tsunoda. Tel +81 742 43 5143. Fax: +81 742 43 5393. e-mail address: tsunoda@nara.kindai.ac.jp
Get access Rights & Permissions [Opens in a new window]

Summary

Somatic cell nuclear-transferred (SCNT) oocytes have a high potential for development in vitro, but a large proportion of embryos that are transferred to recipients is aborted before parturition. The precise mechanism for the high abortion rate is unknown, but abnormal placenta formation is frequently observed in SCNT-cloned pregnancies. The present study examined the effects of treating the recipients with cyclosporin A (CsA), an immunoprotectant, on the proportion of fetuses resulting from SCNT-cloned pregnancies. Cloned embryos developed from enucleated oocytes and receiving cumulus cells from F1 (C57BL/6 × DBA, H-2b/d) females were transferred to outbred ICR (in which the H-2 complex was not fixed) recipient females. Each recipient received an intraperitoneal injection of CsA or vehicle. Compared with vehicle, administration of CsA to recipients on day 4.5 of pregnancy significantly increased the proportion of fetuses observed on day 10.5. The proportion of fetuses at day 18.5 of pregnancy in recipients receiving CsA treatment was slightly higher than that in controls. This study is the first to report that CsA administration increases the proportion of fetuses resulting from SCNT-cloned pregnancies.

Keywords

CsANuclear transferSomatic cells
Type
Research Article
Information
Zygote , Volume 20 , Issue 3 , August 2012 , pp. 261 - 267
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aston, K.I., Li, G.P., Hicks, B.A., Sessions, B.R., Davis, A.P., Winger, Q.A., Rickords, L.F., Stevens, J.R. & White, K.L. (2009). Global gene expression analysis of bovine somatic cell nuclear transfer blastocysts and cotyledons. Mol. Reprod. Dev. 76, 471–82.CrossRefGoogle ScholarPubMed
Bauersachs, S., Ulbrich, S.E., Zakhartchenko, V., Minten, M., Reichenbach, H.D., Blum, H., Spencer, T.E. & Wolf, E. (2009). The endometrium responds differently to cloned versus fertilized embryos. Proc. Natl. Acad. Sci. USA 106, 5681–6.CrossRefGoogle ScholarPubMed
Billington, W.D. (1993). Species diversity in the immunogenetic relationship between mother and fetus: Is trophoblast insusceptibility to immunological destruction the only essential common feature for the maintenance of allogeneic pregnancy. Exp. Clin. Immunogenet. 10, 73–84.Google ScholarPubMed
Blois, S.M., Albam Soto, C.D., Tometten, M., Klappm, B.F., Margnim, R.A. & Arck, P.C. (2004). Lineage, maturity, and phenotype of uterine murine dendritic cells throughout gestation indicate a protective role in maintaining pregnancy. Biol. Reprod. 70, 1018–23.CrossRefGoogle ScholarPubMed
Campbell, K.H.S., Fisher, P., Chen, W.C., Choi, I., Kelly, R.D., Lee, J.H. & Xhu, J. (2007). Somatic cell nuclear transfer: past, present and future perspectives. Theriogenology 68S, S214–37.CrossRefGoogle Scholar
Chaouat, G., Kiger, N. & Wegmann, T.G. (1983). Vaccination against spontaneous abortion in mice. J. Reprod. Immunol. 5, 389–92.CrossRefGoogle ScholarPubMed
Crabtree, G.R. & Olson, E.N. (2002). NFAT signaling: choreographing the social lives of cells. Cell 109, S67–79.CrossRefGoogle ScholarPubMed
Davies, C.J. (2007). Why is the fetal allograft not rejected? J. Anim. Sci. 85, E32–5.CrossRefGoogle Scholar
Davies, C.J., Hill, J.R., Edwards, J.L., Schrick, F.N., Fisher, P.J., Eldridge, J.A. & Schlafer, D.H. (2004). Major histocompatibility antigen expression on the bovine placenta: its relationship to abnormal pregnancies and retained placenta. Anim. Reprod. Sci. 82–83, 267–80.CrossRefGoogle ScholarPubMed
Du, M.R., Dong, L., Zhou, W.H., Yan, F.T. & Li, D.J. (2007). Cyclosporin a improves pregnancy outcome by promoting functions of trophoblasts and inducing maternal tolerance to the allogeneic fetus in abortion-prone mating in the mouse. Biol. Reprod. 76, 906–14.CrossRefGoogle Scholar
Erbach, G.T., Lawitts, J.A., Papaioannou, V.E. & Biggers, J.D. (1994). Differential growth of the mouse preimplantation embryo in chemically defined media. Biol. Reprod. 50, 1027–33.CrossRefGoogle ScholarPubMed
Hill, J.R., Schlafer, D.H., Fisher, P.J. & Davis, C.J. (2002). Abnormal expression of trophoblast major histocompatibility complex class I antigens in cloned bovine pregnancy is associated with a pronounced endometrial lymphocytic response. J. Immunol. 138, 2481–7.Google Scholar
Jenkinson, E.J. & Searle, R.F. (1979). Ia antigen expression on the developing mouse embryo and placenta. J. Reprod. Immunol. 1, 3–10.CrossRefGoogle ScholarPubMed
Kato, Y., Tani, T., Sotomaru, Y., Kurokawa, K., Kato, J., Doguchi, H., Yasue, H. & Tsunoda, Y. (1998). Eight calves cloned from somatic cells of a single adult. Science 282, 2095–8.CrossRefGoogle ScholarPubMed
Kishigami, S., Mizutani, E., Ohta, H., Hikichi, T., Mizutani, E., Bui, H.T. & Wakayama, T. (2006). Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer. Biochem. Biophys. Res. Commun. 340, 183–9.CrossRefGoogle ScholarPubMed
Laird, S.M., Tuckerman, E.M., Cork, B.A., Linjawi, S., Blakemore, A.I. & Li, T.C. (2003). A review of immune cells and molecules in women with recurrent miscarriage. Hum. Reprod. Update 9, 163–74.CrossRefGoogle ScholarPubMed
Li, X., Kato, Y. & Tsunoda, Y. (2005). Comparative analysis of development-related gene expression in mouse preimplantation embryos with different developmental potential. Mol. Reprod. Dev. 72, 152–60.CrossRefGoogle ScholarPubMed
Liu, J., Sakane, T. & Tsunematsu, T. (1992). The effects of FK-506 and cyclosporine A on the proliferation of PHA-stimulated T cells in response to IL-2, IL-4 or IL-6. Int. Arch. Immunol. 98, 293–8.CrossRefGoogle ScholarPubMed
Meng, Q., Wang, M., Stanca, C.A., Bodo, S. & Dinnyes, A. (2008). Cotransfer of parthenogenetic embryos improves the pregnancy and implantation of nuclear transfer embryos in mouse. Cloning Stem Cells 10, 429–34.CrossRefGoogle Scholar
Nan, C.L., Lei, Z.L., Zhao, Z.J., Shi, L.H., Quyang, Y.C., Song, X.F., Sun, Q.Y. & Chen, D.Y. (2007). Increased Th1/Th2 (IFN-gamma/IL-4) cytokine mRNA ratio of rat embryos in the pregnant mouse uterus. J. Reprod. Dev. 53, 219–28.CrossRefGoogle ScholarPubMed
Numabe, T., Oikawa, T., Kikuchi, T. & Horiuchi, T. (2000). Production efficiency of Japanese black calves by transfer of bovine embryos produced in vitro. Theriogenology 54, 1409–20.CrossRefGoogle ScholarPubMed
Palmieri, C., Loi, P., Ptak, G. & Della Salda, L. (2008). Review paper; a review of the pathology of abnormal placentae of somatic cell nuclear transfer clone pregnancies in cattle, sheep and mice. Vet. Pathol. 45, 865–80.CrossRefGoogle ScholarPubMed
Perrier d'Hauterive, S., Berndt, S. & Tsampalas, M. (2007). Dialogue between blastocyst hCG and endometrial LH/hCG receptor; which role in implantation? Gynecol. Obst. Invest. 64, 156–60.Google ScholarPubMed
Pfister-Genskow, M., Myers, C., Childs, L.A., Lacson, J.C., Patterson, T., Betthuser, J.M., Gouelek, P.J., Koppang, R.W., Lange, G., Fisher, P., Watt, S.R., Forsberg, E.J., Zheng, Y., Leno, G.H., Schultz, R.M., Liu, B., Chetia, C.C., Yang, X., Hoeschele, I. & Eilertsen, K.J. (2005). Identification of differentially expressed genes in individual bovine preimplantation embryos produced by nuclear transfer: improper reprogramming of genes required for development. Biol. Reprod. 72, 546–55.CrossRefGoogle ScholarPubMed
Raghupathy, R. (2001). Pregnancy; success and failure within the Th1/Th2/Th3 paradigm. Semin. Immunol. 13, 219–27.CrossRefGoogle ScholarPubMed
Rybouchkin, A., Kato, Y. & Tsunoda, Y. (2006). Role of histone acetylation in reprogramming of somatic nuclei following nuclear transfer. Biol. Reprod. 74, 1083–9.CrossRefGoogle ScholarPubMed
Schreiber, S.L. & Crabtree, G.R. (1992). The mechanism of action of cyclosporin A and FK506. Immunol. Today 13, 136–42.CrossRefGoogle ScholarPubMed
Shomer, B., Toder, V., Egorov, I. & Ehrlich, R. (1998). Expression of allogeneic MHC class I antigens by transgenic mouse trophoblast does not interfere with the normal course of pregnancy. Transgenic Res. 7, 343–55.CrossRefGoogle Scholar
Sketris, I., Yatscoff, R., Keown, P., Canafax, D.M., First, M.R., Holt, D.W., Schroeder, T.J. & Wright, M. (1995). Optimizing the use of cyclosporine in renal transplantation. Clin. Biochem. 28, 195–211.CrossRefGoogle ScholarPubMed
Takakuwa, K., Goto, S., Hasegawa, I., Ueda, H., Kanagawa, K., Takeuchi, S. & Tanaka, K. (1990). Results of immunotherapy on patients with unexplained recurrent abortion: a beneficial treatment for patients with negative blocking antibodies. Am. J. Reprod. Immunol. 23, 37–41.CrossRefGoogle ScholarPubMed
Tsuji, Y., Kato, Y. & Tsunoda, Y. (2009). The developmental potential of mouse somatic cell nuclear-transferred oocytes treated with trichostatin A and 5-aza-2'-deoxycytidine. Zygote 17, 109–15.CrossRefGoogle ScholarPubMed
Tsunoda, Y. & Kato, Y. (1995). Development of enucleated mouse oocytes receiving PDGF or FGF treated fetal male germ cells after activation with electrical stimulation. J. Reprod. Dev. 41, 71–5.CrossRefGoogle Scholar
Tsunoda, T., Iritani, A. & Nishikawa, Y. (1978). Immunological investigations of the cause of the limited development of rat eggs transferred into pregnant rabbit uterus. Jap. J. Zootech. Sci. 49, 96–103.Google Scholar
Voland, J.R., Becker, C. & Hooshmand, F. (1994). Overexpression of class I MHC in muring trophoblast and increased rates of spontaneous abortions. In: Immunology of Reproduction (Hunt, J.S. ed.), Serono Symposia, USA Norwell, Massachusetts, Springer-Verlag.Google Scholar
Wakayama, T., Perry, A.C., Zuccotti, M., Johnson, K.R. & Yanagimachi, R. (1998). Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394, 369–74.CrossRefGoogle ScholarPubMed
Zhou, W-H., Dong, L. & Du, M-R. (2008). Cyclosporin A improves murine pregnancy outcome in abortion-prone mating: involvement of CD80/86 and CD28/CTLA-4. Reproduction 135, 385–95.CrossRefGoogle ScholarPubMed