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Adipose-derived stem cells transplantation improves endometrial injury repair

Published online by Cambridge University Press:  27 August 2019

Xiaowen Shao Open the ORCID record for Xiaowen Shao [Opens in a new window] ,
Guihai Ai ,
Lian Wang ,
Jinlong Qin ,
Yue Li ,
Huici Jiang ,
Tingting Zhang ,
Linlin Zhou ,
Zhengliang Gao  and
Jiajing Cheng
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Xiaowen Shao
Affiliation:
Department of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China
Guihai Ai
Affiliation:
Department of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China
Lian Wang
Affiliation:
Department of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China
Jinlong Qin
Affiliation:
Department of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China
Yue Li
Affiliation:
Department of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China
Huici Jiang
Affiliation:
Department of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China
Tingting Zhang
Affiliation:
Department of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China
Linlin Zhou
Affiliation:
Department of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China
Zhengliang Gao
Affiliation:
Lifeng Institute of Regenerative Medicine, Tongji University, Shanghai, 200092, China
Jiajing Cheng
Affiliation:
Department of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China
Zhongping Cheng*
Affiliation:
Department of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China
*
Address for correspondence: Zhongping Cheng. Department of Gynecology and Obstetrics, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, No. 301, Yan Chang Road, 200072, Shanghai, China. Tel: +86 21 66300588. E-mail: mdcheng18@tongji.edu.cn
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Summary

Endometrial injury is an important cause of intrauterine adhesion (IUA), amenorrhea and infertility in women, with limited effective therapies. Recently, stem cells have been used in animal experiments to repair and improve injured endometrium. To date, our understanding of adipose-derived stem cells (ADSCs) in endometrial injury repair and their further therapeutic mechanisms is incomplete. Here, we examined the benefit of ADSCs in restoration of injured endometrium by applying a rat endometrial injury model. The results revealed by immunofluorescence showed that green fluorescent protein (GFP)-labelled ADSCs can differentiate into endometrial epithelial cells in vivo. At 30 days after ADSCs transplantation, injured endometrium was significantly improved, with increased microvessel density, endometrial thickness and glands when compared with the model group. Furthermore, the fertility of rats with injured endometrium in ADSCs group was improved and had a higher conception rate (60% vs 20%, P = 0.014) compared with the control phosphate-buffered saline (PBS) group. However, there was no difference in the control group compared with the sham group. In addition, expression levels of the oestrogen receptor Eα/β (ERα, ERβ) and progesterone receptor (PR) detected by western blot and enzyme-linked immunosorbent assay (ELISA) were higher in the ADSCs group than in the PBS group. Taken together, these results suggested that ADSC transplantation could improve endometrial injury as a novel therapy for IUA.

Keywords

Adipose-derived stem cellsEndometrial injuryRegenerationStem cell transplantation
Type
Research Article
Information
Zygote , Volume 27 , Issue 6 , December 2019 , pp. 367 - 374
Copyright
© Cambridge University Press 2019 

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Footnotes

*

These authors contributed equally to this work.

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