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The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls

  • João D.A. Losano (a1), Daniel S.R. Angrimani (a2), Bruno R. Rui (a1), Luana C. Bicudo (a1), Andressa Dalmazzo (a1), Bárbara C.S. Silva (a1), Carlos H.C. Viana (a1), Camilla M. Mendes (a1), Mayra E.O.A. Assumpção (a1), Valquiria H. Barnabe (a1) and Marcilio Nichi (a1)...


The cryopreservation of epididymal sperm is an important technique that allows genetic material to be preserved, even post mortem. However, cryopreservation leads to increased oxidative stress and impaired sperm viability. Polyunsaturated fatty acid (PUFA) supplementation may improve certain sperm characteristics, but it also makes sperm more susceptible to oxidative stress, therefore adding antioxidants that counteract oxidative stress has become an option. In this context, this study aimed to evaluate the effect of the interaction between docosahexaenoic acid (DHA) and antioxidants on the quality after the cryopreservation of epididymal bull sperm. Twenty epididymides were collected after slaughter, and epididymal sperm was cryopreserved with bovine extender supplemented with docosahexaenoic acid (DHA), glutathione peroxidase (GPx) and superoxide dismutase (SOD). We verified an improvement in motility in the group that was treated only with DHA 5 µM and a concentration-dependent effect on susceptibility to lipid peroxidation that was associated with DHA concentration (1 µM, 5 µM or 10 µM). Moreover, treatment with DHA (5 µM) and SOD (20 IU/ml) resulted in higher sperm motility. Thus, the association between DHA (5 µM) and SOD (20 IU/ml) appears to be an option for increased epididymal sperm features in bulls.


Corresponding author

All correspondence to: Daniel S.R. Angrimani. Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Orlando Marques de Paiva, 87-05508-270, São Paulo, Brazil. Tel: +55 11 30917914. Fax: +55 11 30917914. E-mail:


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These authors contributed equally to this work.



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