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Activin-A promotes the development of goat isolated secondary follicles in vitro

  • Cleidson Manoel Gomes da Silva (a1), Simone Vieira Castro (a2), Luciana Rocha Faustino (a2), Giovanna Quintino Rodrigues (a2), Ivina Rocha Brito (a2), Rafael Rossetto (a2), Márcia Viviane Alves Saraiva (a2), Claudio Cabral Campello (a2), Carlos Henrique Lobo (a3), Carlos Eduardo Azevedo Souza (a3), Arlindo de Alencar Araripe Moura (a3), Mariana Aragão Matos Donato (a4), Christina Alves Peixoto (a4) and José Ricardo de Figueiredo (a2)...


The role of activin-A in follicular development and on the mRNA expression levels of different genes in goat secondary follicles was evaluated. Goat secondary follicles (≥150 μm) were cultured for 18 days under control conditions or with the addition of either 50 or 100 ng/ml activin-A (Experiment 1). The mRNA levels for the genes that code for activin-A, ActR-IA, ActR-IB, ActR-IIA, ActR-IIB, follicle stimulating hormone receptor (FSH-R) and P450 aromatase were measured in each condition (Experiment 2). We observed that after 6 days of culture, the antrum formation rate was higher in cultures with added activin-A than in the cultured control (P < 0.05). The addition of 50 ng/ml activin-A increased the follicular growth rate in the final third of the culture (days 12–18), resulting in a higher percentage of meiosis resumption (P < 0.05). On day 6, the addition of activin-A (50 ng/ml) increased the levels of ActR-IA mRNA compared with the cultured control (P < 0.05). After 18 days, the addition of 50 ng/ml activin-A significantly increased the levels of its own mRNA compared with the non-cultured control. Moreover, this treatment reduced the mRNA levels of P450 aromatase in comparison with the cultured control (P < 0.05). Higher levels of P450 aromatase mRNA were found for both activin-A treatments compared with the non-cultured control (P < 0.05). No difference in estradiol levels was detected among any of the tested treatments. In conclusion, the addition of activin-A to culture medium stimulated early antrum formation as well as an increase in the daily follicular growth rate and the percentage of meiosis resumption.


Corresponding author

All correspondence to: Cleidson Manoel Gomes da Silva. Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana, 1700, Campus do Itaperi, CEP: 60740–000, Fortaleza–CEBrasil. Tel: +55 85 3101 9852. Fax: +55 85 3101 9840. e-mail:


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Activin-A promotes the development of goat isolated secondary follicles in vitro

  • Cleidson Manoel Gomes da Silva (a1), Simone Vieira Castro (a2), Luciana Rocha Faustino (a2), Giovanna Quintino Rodrigues (a2), Ivina Rocha Brito (a2), Rafael Rossetto (a2), Márcia Viviane Alves Saraiva (a2), Claudio Cabral Campello (a2), Carlos Henrique Lobo (a3), Carlos Eduardo Azevedo Souza (a3), Arlindo de Alencar Araripe Moura (a3), Mariana Aragão Matos Donato (a4), Christina Alves Peixoto (a4) and José Ricardo de Figueiredo (a2)...


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