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Using Electrolyte Leakage to Detect Soybean (Glycine max) Cultivars Sensitive to Sulfentrazone

Published online by Cambridge University Press:  20 January 2017

Zhaohu Li ,
Robert H. Walker ,
Glenn Wehtje  and
H. Gary Hancock
Zhaohu Li
Affiliation:
Department of Agronomy and Soils, Alabama Agricultural Experiment Station, Auburn University, Auburn, AL 36849-5412
Robert H. Walker*
Affiliation:
Department of Agronomy and Soils, Alabama Agricultural Experiment Station, Auburn University, Auburn, AL 36849-5412
Glenn Wehtje
Affiliation:
Department of Agronomy and Soils, Alabama Agricultural Experiment Station, Auburn University, Auburn, AL 36849-5412
H. Gary Hancock
Affiliation:
FMC Corporation, Hamilton, GA 31811
*
Corresponding author's E-mail: rwalker@acesag.auburn.edu.
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Abstract

Laboratory studies were conducted to determine if electrolyte leakage from either leaf tissue, germinating seeds, or excised roots correlated with previously established soil-applied field response of soybean cultivars and target weeds to sulfentrazone. Sulfentrazone-induced electrolyte leakage from leaf tissue of coffee senna (sensitive), sicklepod (tolerant), and soybean cultivars ‘Asgrow 6785’ and ‘Carver’ (sensitive) and ‘Stonewall’ and ‘DPL 3606’ (tolerant) was monitored over time. Electrolyte leakage from leaf tissues, caused by 25 ppm (65 μM) sulfentrazone, agreed directly with the known response of these weeds, but response of the four soybean cultivars was equivalent. Furthermore, sulfentrazone-induced electrolyte leakage from leaf tissue of Asgrow 6785 and Stonewall was not affected by sulfentrazone concentration as high as 100 ppm (258 μM) nor by light intensity (4 and 120 μmol/m2/s photosynthetically active radiation). For germinating seeds, sulfentrazone-induced electrolyte leakage was also independent of soybean cultivar. In contrast, electrolyte leakage from excised roots of germinal soybean seedlings did concur directly with the previously established cultivar sensitivity to soil-applied sulfentrazone. Results indicate that electrolyte leakage from excised roots of soybean germinal seedlings can be used to assess cultivar sensitivity to soil-applied sulfentrazone.

Keywords

Sulfentrazone, coffee senna, Cassia occidentalis L. #3 CASOCsicklepod, Senna obtusifolia L. # CASOBsoybean, Glycine max (L.) Merr. ‘Asgrow 6785’, ‘Carver’, ‘Stonewall’, ‘DPL 3606’Herbicide tolerancemembrane leakageProtoxlight intensityIe, index of relative electrolyte leakagePAR, photosynthetically active radiationProtogen, protoporphyrinogenProto IX, protoporphyrinProtox, protoporphyrinogen oxidase
Type
Research Article
Information
Weed Technology , Volume 14 , Issue 4 , December 2000 , pp. 699 - 704
Copyright
Copyright © Weed Science Society of America 

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