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Genetic Analysis of Glufosinate Resistance in Crosses Between Transformed Rice (Oryza sativa) and Red Rice (Oryza sativa)

Published online by Cambridge University Press:  12 June 2017

Sujatha Sankula ,
Michael P. Braverman  and
James H. Oard
Sujatha Sankula
Affiliation:
Department of Plant Pathology and Crop Physiology, 302, Life Sciences Building
Michael P. Braverman
Affiliation:
Department of Plant Pathology and Crop Physiology, 302, Life Sciences Building
James H. Oard
Affiliation:
Department of Agronomy, 104, M. B. Sturgis Hall, Louisiana State University, Baton Rouge, LA 70803
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Abstract

Reciprocal controlled crosses were made in the greenhouse between Gulfmont rice transformed with the bialaphos resistance (BAR) gene and red rice and BAR-transformed Koshihikari rice and red rice to assess the inheritance of glufosinate resistance. All F1 plants were resistant to 2.2 kg ai/ha glufosinate. Ammonia accumulation as a measure of glufosinate resistance in the F1 hybrids was assayed at 4 and 8 days after treatment (DAT). Ammonia accumulation in hybrids 4 DAT was similar to glufosinate treated, transformed rice, while treated nontransformed plants accumulated 14 to 23 times more ammonia compared with the hybrids. The nature of inheritance of glufosinate resistance in F2 rice plants was studied by a glufosinate dip test, a spray test, and ammonia assay. All three tests confirmed that glufosinate resistance, as influenced by the BAR gene, segregated in a 3 (resistant): 1 (susceptible) ratio.

Keywords

Glufosinate, 2-amino-4-(hydroxymethylphosphinyl)butanoic acidred rice, Oryza sativa L. #3 ORYSArice, Oryza sativa L. ‘Gulfmont’ and ‘Koshihikari.'Ammonia assayBAR geneherbicide-resistant cropsinheritancetransformed riceORYSABAR, bialaphos resistanceDAT, days after treatmentGUS, galacturonidaseHm, hygromycin resistance genePAT, phosphinothricin acetyl transferaseWAT, weeks after treatment
Type
Research
Information
Weed Technology , Volume 12 , Issue 2 , June 1998 , pp. 209 - 214
Copyright
Copyright © 1998 by the Weed Science Society of America 

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