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Transformation of Alachlor by Microbial Communities

  • Hone L. Sun (a1), Thomas J. Sheets (a2) and Frederick T. Corbin (a3)


A mixed microbial culture able to transform alachlor at a concentration of 50 μg ml-1 was obtained from alachlor-treated soil after an enrichment period of 84 days. The microbial community was composed of seven strains of bacteria. No single isolate was able to utilize alachlor as a sole source of carbon. There was no alachlor left in the enriched culture after a 14-day incubation, but only 12% of the 14C-ring-labeled alachlor was converted to 14CO2 through ring cleavage during 14 days in the basal medium amended with alachlor as a sole carbon source. The presence of sucrose as an alternative carbon source decreased the mineralization potential of the enriched culture, but sucrose increased the mineralizing ability of a three-member mixed culture. Thin-layer chromatographic analysis showed that there were four unidentified metabolites of alachlor produced by the enriched culture. Sucrose decreased the amount of two of the four metabolites. The absence of a noticeable decline in radioactivity beyond the initial 12% suggested that the side chain of alachlor was utilized as carbon source by the enriched culture. Little difference in radioactivity between growth medium and cell-free supernatant of the growth medium suggested that the carbon in the ring was not incorporated into the cells of the degrading microorganisms.



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1. Alexander, M. 1981. Biodegradation of chemicals of environmental concern. Science 211:132138.
2. Baker, J. L. and Laflen, J. M. 1979. Runoff losses of surface-applied herbicides as affected by wheel tracks and incorporation. J. Environ. Qual. 8:602607.
3. Beestman, G. B. and Deming, J. M. 1974. Dissipation of acetanilide herbicides from soils. Agron. J. 66:308311.
4. Bollag, J. M., McGahen, L. L., Minard, R. D., and Liu, S. Y. 1986. Bioconversion of alachlor in an anaerobic stream sediment. Chemosphere 15:153162.
5. Bordeleau, L. M. and Bartha, R. 1968. Ecology of a pesticide transformation: synergism of two soil fungi. Soil Biol. Biochem. 3:281284.
6. Corbin, F. T. and Swisher, B. A. 1986. Radioisotope techniques. Pages 265276 in Camper, N. D., ed. Research Methods in Weed Science. South. Weed Sci., Soc., Champaign, IL.
7. Daughton, C. G. and Hsieh, D.P.H. 1977. Parathion utilization by bacterial symbionts in a chemostat. Appl. Environ. Microbiol. 34:175184.
8. Eastin, E. F. 1986. Absorption, translocation and degradation of herbicides by plants. Pages 277290 in Camper, N. D., ed. Research Methods in Weed Science. South. Weed Sci., Soc, Champaign, IL.
9. Feinberg, E. L., Ramage, P.I.N., and Trudgill, P. W. 1980. The degradation of n-alkylcycloalkanes by a mixed bacteria culture. J. Gen. Microbiol. 121:507511.
10. Hsu, T.-S. and Bartha, R. 1979. Accelerated mineralization of two organophosphate insecticides in the rhizosphere. Appl. Environ. Microbiol. 45:14591465.
11. Kaufman, D. D. and Blake, J. 1973. Microbial degradation of several acetamide, acylamide, carbamate, toluidine, and urea pesticides. Soil Biol. Biochem 5:297308.
12. Lappin, H. M., Greaves, M. P., and Slater, J. H. 1985. Degradation of the herbicide mecoprop [2-(2-methyl 4-chlorophenoxy)-propionic acid] by a synergistic community. Appl. Environ. Microbiol. 49:429433.
13. Lappin-Scott, H. M., Greaves, M. P., and Slater, J. H. 1986. Degradation of the herbicide mecoprop by a microbial community. Pages 211218 in Jensen, V., Kjoller, A., and Sorensen, L. H., eds. Microbial Communities in Soil. Elsevier Applied Science Publishers, London and New York.
14. MacFaddin, J. F. 1976. Biochemical Tests for Identification of Medical Bacteria. The Williams and Wilkins Co., Baltimore, MD. 312 pp.
15. Novick, N. J. and Alexander, M. 1985. Cometabolism of low concentrations of propachlor, alachlor, and cycloate in sewage and lake water. Appl. Environ. Microbiol. 49:737743.
16. Novick, N. J., Mukherjee, R., and Alexander, M. 1986. Metabolism of alachlor and propachlor in suspensions of pretreated soils and in samples from ground water aquifers. J. Agric. Food Chem. 34:721725.
17. Reanney, D. C., Gowl, P. C., and Slater, J. H. 1983. Genetic interactions among microbial communities. Pages 379421 in Slater, J. H., Whittenbury, R. W., and Wimpenny, J. W., eds. Microbes in Their Natural Environments. Society for General Microbiology Symposium 34. Cambridge Univ. Press, Cambridge, UK.
18. SAS Institute. 1982. SAS User's Guide: Basics. 1982. SAS Inst., Inc., Cary, NC.
19. Senior, E., Bull, A. T., and Slater, J. H. 1976. Enzyme evolution in a microbial community growing on the herbicide Dalapon. Nature (London) 263:476479.
20. Slater, J. H. and Bull, A. T. 1982. Environmental microbiology: biodegradation. Philos. Trans. R. Soc. Lond. 297:575597.
21. Slater, J. H. and Godwin, D. 1980. Microbial adaption and selection. Pages 137160 in Ellwood, D. C., Hedger, J. N., Latham, M. J., Lynch, J. M., and Slater, J. H., eds. Contemporary Microbial Ecology. Academic Press, Inc., London.
22. Slater, J. H. and Sommerville, H. J. 1979. Microbial aspects of waste treatment with particular attention to the degradation of organic compounds. Pages 221261 in Bull, A. T., Ellwood, D. C., and Ratledge, C. R., eds. Microbial Technology: Current Status, Future Prospects. Cambridge Univ. Press, Cambridge, UK.
23. Smith, A. E. and Phills, D. V. 1975. Degradation of alachlor by Rhizoctonia solani . Agron. J. 67:347349.
24. Steel, R.G.D. and Torrie, J. H. 1984. Principles and Procedures of Statistics. 2nd ed. McGraw-Hill Book Co., New York. 633 pp.
25. Tiedje, J. M. and Hagedorn, M. L. 1975. Degradation of alachlor by a soil fungus, Chaetomium globosum . J. Agric. Food Chem. 23:7781.
26. Wu, T. L. 1980. Dissipation of the herbicides atrazine and alachlor in a Maryland cornfield. J. Environ. Qual. 9:459465.
27. Wu, T. L., Correll, D. L., and Remenapp, H.E.H. 1983. Herbicide runoff from experimental watersheds. J. Environ. Qual. 12:330336.
28. Zimdahl, R. L. and Clark, S. K. 1982. Degradation of three acetanilide herbicides in soil. Weed Sci. 30:545548.


Transformation of Alachlor by Microbial Communities

  • Hone L. Sun (a1), Thomas J. Sheets (a2) and Frederick T. Corbin (a3)


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