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Target-Site Resistance to Fenoxaprop-P-ethyl in Keng Stiffgrass (Sclerochloa kengiana) from China

  • Haitao Gao (a1), Jiaxing Yu (a1), Lang Pan (a1), Xibao Wu (a1) and Liyao Dong (a1)...

Abstract

Keng stiffgrass is a serious farmland grass weed distributed globally in winter wheat fields and rice–wheat double-cropping areas. The intensive use of acetyl-CoA carboxylase (ACCase)-inhibiting herbicides has led to the evolution of resistance in a growing number of grass weeds. In this study, whole-plant pot bioassay experiments were conducted to establish that a Keng stiffgrass population from eastern China, JYJD-2, has evolved high-level resistance to fenoxaprop-P-ethyl and moderate resistance to quizalofop-P-ethyl and pinoxaden. Using the derived cleaved amplified polymorphic sequence method, a tryptophan-to-cysteine mutation at codon position 1999 (W1999C) was detected in the ACCase gene of the resistant population JYJD-2. Of the 100 JYJD-2 plants tested, we found 47 heterozygous resistant and 53 homozygous sensitive individuals. In vitro ACCase assays revealed that the IC50 value of the ACCase activity of the resistant population JYJD-2 was 6.48-fold higher than that of the susceptible population JYJD-1. To the best of our knowledge, this is the first report of the occurrence of W1999C mutation in the ACCase gene of fenoxaprop-P-ethyl–resistant Keng stiffgrass. This study confirmed the resistance of Keng stiffgrass to the ACCase inhibitor fenoxaprop-P-ethyl, cross-resistance to other ACCase inhibitors, and the resistance being conferred by specific ACCase point mutations at amino acid position 1999.

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*Corresponding author's E-mail: dly@njau.edu.cn

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Associate Editor for this paper: Vijay Nandula, USDA–ARS.

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Beckie, HJ, Tardif, FJ (2012) Herbicide cross resistance in weeds. Crop Prot 35:1528
Beckie, HJ, Warwick, SI, Sauder, CA (2011) Basis for herbicide resistance in Canadian populations of wild oat (Avena fatua). Weed Sci 60:1018
Bi, Y, Liu, W, Guo, W, Li, L, Yuan, G, Du, L, Wang, J (2016) Molecular basis of multiple resistance to ACCase- and ALS-inhibiting herbicides in Alopecurus japonicus from China. Pestic Biochem Physiol 126:2227
Cui, H, Li, X, Wang, G, Wang, J, Wei, S, Cao, H (2012) Acetolactate synthase proline (197) mutations confer tribenuron-methyl resistance in Capsella bursa-pastoris populations from China. Pestic Biochem Physiol 102:229232
Délye, C (2005) Weed resistance to acetyl coenzyme A carboxylase inhibitors: an update. Weed Sci 53:728746
Délye, C, Calmès, É, Matéjicek, A (2002a) SNP markers for black-grass (Alopecurus myosuroides Huds.) genotypes resistant to acetyl CoA-carboxylase inhibiting herbicides. Theor Appl Genet 104:11141120
Délye, C, Matéjicek, A, Gasquez, J (2002b) PCR-based detection of resistance to acetyl–CoA carboxylase–inhibiting herbicides in black–grass (Alopecurus myosuroides Huds) and ryegrass (Lolium rigidum Gaud). Pest Manag Sci 58:474478
Délye, C, Pernin, F, Michel, S (2011) “Universal” PCR assays detecting mutations in acetyl-coenzyme A carboxylase or acetolactate synthase that endow herbicide resistance in grass weeds. Weed Res 51:353362
Délye, C, Zhang, XQ, Chalopin, C, Michel, S, Powles, SB (2003) An isoleucine residue within the carboxyl-transferase domain of multidomain acetyl-coenzyme A carboxylase is a major determinant of sensitivity to aryloxyphenoxypropionate but not to cyclohexanedione inhibitors. Plant Physiol 132:17161723
Délye, C, Zhang, XQ, Michel, S, Matéjicek, A, Powles, SB (2005) Molecular bases for sensitivity to acetyl-coenzyme A carboxylase inhibitors in black-grass. Plant Physiol 137:794806
Heap, I (2016) The International Survey of Herbicide Resistant Weeds. http://www.weedscience.com. Accessed: March 16, 2016
Heap, I, Knight, R (1986) The occurrence of herbicide cross-resistance in a population of annual ryegrass (Lolium rigidum) resistant to diclofop-methyl. Aust J Agric Res 37:149156
Hochberg, O, Sibony, M, Rubin, B (2009) The response of ACCase-resistant Phalaris paradoxa populations involves two different target site mutations. Weed Res 49:3746
Jang, S, Marjanovic, J, Gornicki, P (2013) Resistance to herbicides caused by single amino acid mutations in acetyl-CoA carboxylase in resistant populations of grassy weeds. New Phytol 197:11101116
Kaundun, SS (2010) An aspartate to glycine change in the carboxyl transferase domain of acetyl-CoA carboxylase and non-target-site mechanism(s) confer resistance to ACCase inhibitor herbicides in a Lolium multiflorum population. Pest Manag Sci 66:12491256
Kaundun, SS, Bailly, GC, Dale, RP, Hutchings, SJ, McIndoe, E (2013) A novel W1999S mutation and non-target site resistance impact on acetyl-CoA carboxylase inhibiting herbicides to varying degrees in a UK Lolium multiflorum population. PLoS ONE 8:e58012
Li, L, Du, L, Liu, W, Yuan, G, Wang, J (2014) Target-site mechanism of ACCase-inhibitors resistance in American sloughgrass (Beckmannia syzigachne Steud.) from China. Pestic Biochem Physiol 110:5762
Liu, W, Harrison, DK, Chalupska, D, Gornicki, P, O’Donnell, CC, Adkins, SW, Haselkorn, R, Williams, RR (2007) Single-site mutations in the carboxyltransferase domain of plastid acetyl-CoA carboxylase confer resistance to grass-specific herbicides. Proc Natl Acad Sci USA 104:36273632
Neff, MM, Turk, E, Kalishman, M (2002) Web-based primer design for single nucleotide polymorphism analysis. Trends Genet 18:613615
Pan, L, Li, J, Xia, W, Zhang, D, Dong, L (2015a) An effective method, composed of LAMP and dCAPS, to detect different mutations in fenoxaprop-P-ethyl-resistant American sloughgrass (Beckmannia syzigachne Steud.) populations. Pestic Biochem Physiol 117:18
Pan, L, Li, J, Zhang, W, Dong, L (2015c) Detection of the I1781L mutation in fenoxaprop-P-ethyl-resistant American sloughgrass (Beckmannia syzigachne Steud.), based on the loop-mediated isothermal amplification method. Pest Manag Sci 71:123130
Pan, L, Li, J, Zhang, T, Zhang, D, Dong, L (2015b) Cross-resistance patterns to acetyl coenzyme A carboxylase (ACCase) inhibitors associated with different ACCase mutations in Beckmannia syzigachne . Weed Res 55:609620
Petit, C, Bay, G, Pernin, F, Delye, C (2010) Prevalence of cross- or multiple resistance to the acetyl-coenzyme A carboxylase inhibitors fenoxaprop, clodinafop and pinoxaden in black-grass (Alopecurus myosuroides Huds.) in France. Pest Manag Sci 66:168177
Powles, SB, Yu, Q (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol 61:317347
Rao, N, Dong, L, Li, J, Zhang, H (2008) Influence of environmental factors on seed germination and seedling emergence of American Sloughgrass (Beckmannia syzigachne). Weed Sci 56:529533
Scarabel, L, Panozzo, S, Varotto, S, Sattin, M (2011) Allelic variation of the ACCase gene and response to ACCase-inhibiting herbicides in pinoxaden-resistant Lolium spp. Pest Manag Sci 67:932941
Shukla, A, Leach, GE, Devine, MD (1997) High-level resistance to sethoxydim conferred by an alteration in the target enzyme, acetyl-CoA carboxylase, in Setaria faberi and Setaria viridis . Plant Physiol Biochem 35:803807
Stoltenberg, DE, Gengenbach, BG (1989) Effect of sethoxydim and haloxyfop on acetyl-coenzyme A carboxylase activity in Festuca species. Weed Sci 37:512516
Tang, W, Zhou, F, Chen, J, Zhou, X (2014) Resistance to ACCase-inhibiting herbicides in an Asia Minor bluegrass (Polypogon fugax) population in China. Pestic Biochem Physiol 108:1620
Tardif, FJ, Powles, SB (1994) Herbicide multiple-resistance in a Lolium rigidum biotype is endowed by multiple mechanisms: isolation of a subset with resistant acetyl-CoA carboxylase. Physiol Plant 91:488494
Warwick, SI, Sauder, CA, Beckie, HJ (2010) Acetolactate synthase (ALS) target-site mutations in ALS inhibitor-resistant Russian thistle (Salsola tragus). Weed Sci 58:244251
Xu, H, Li, J, Zhang, D, Cheng, Y, Jiang, Y, Dong, L (2014a) Mutations at codon position 1999 of acetyl-CoA carboxylase confer resistance to ACCase-inhibiting herbicides in Japanese foxtail (Alopecurus japonicus). Pest Manag Sci 70:18941901
Xu, H, Zhang, W, Zhang, T, Li, J, Wu, X, Dong, L (2014b) Determination of ploidy level and isolation of genes encoding acetyl-CoA carboxylase in Japanese foxtail (Alopecurus japonicus). PLoS ONE 9:e114712
Xu, H, Zhu, X, Wang, H, Li, J, Dong, L (2013) Mechanism of resistance to fenoxaprop in Japanese foxtail (Alopecurus japonicus) from China. Pestic Biochem Physiol 107:2531
Yang, C, Dong, L, Li, J, Moss, SR (2007) Identification of Japanese foxtail (Alopecurus japonicus) resistant to haloxyfop using three different assay techniques. Weed Sci 55:537540
Yu, Q, Ahmad-Hamdani, MS, Han, H, Christoffers, MJ, Powles, SB (2013) Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): insights into resistance evolution in a hexaploid species. Heredity 110:220231
Yu, Q, Cairns, A, Powles, S (2007a) Glyphosate, paraquat and ACCase multiple herbicide resistance evolved in a Lolium rigidum biotype. Planta 225:499513
Yu, Q, Collavo, A, Zheng, MQ, Owen, M, Sattin, M, Powles, SB (2007b) Diversity of acetyl-coenzyme A carboxylase mutations in resistant Lolium populations: evaluation using clethodim. Plant Physiol 145:547558
Yuan, G, Liu, W, Bi, Y, Du, L, Guo, W, Wang, J (2015) Molecular basis for resistance to ACCase-inhibiting herbicides in Pseudosclerochloa kengiana populations. Pestic Biochem Physiol 119:915
Zhang, H, Yang, Z, Shen, Y, Tong, L (2003) Crystal structure of the carboxyltransferase domain of acetyl-coenzyme A carboxylase. Science 299:20642067
Zhang, ZP (2003) Development of chemical weed control and integrated weed management in China. Weed Biol Manag 3:197203

Keywords

Target-Site Resistance to Fenoxaprop-P-ethyl in Keng Stiffgrass (Sclerochloa kengiana) from China

  • Haitao Gao (a1), Jiaxing Yu (a1), Lang Pan (a1), Xibao Wu (a1) and Liyao Dong (a1)...

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