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Target and Non–target site Mechanisms Confer Resistance to Glyphosate in Canadian Accessions of Conyza canadensis

  • Eric R. Page (a1), Christopher M. Grainger (a2), Martin Laforest (a3), Robert E. Nurse (a1), Istvan Rajcan (a2), Jichul Bae (a1) and François J. Tardif (a2)...

Abstract

Glyphosate-resistant populations of Conyza canadensis have been spreading at a rapid rate in Ontario, Canada, since first being documented in 2010. Determining the genetic relationship among existing Ontario populations is necessary to understand the spread and selection of the resistant biotypes. The objectives of this study were to: (1) characterize the genetic variation of C. canadensis accessions from the province of Ontario using simple sequence repeat (SSR) markers and (2) investigate the molecular mechanism (s) conferring resistance in these accessions. Ninety-eight C. canadensis accessions were genotyped using 8 SSR markers. Germinable accessions were challenged with glyphosate to determine their dose response, and the sequences of 5-enolpyruvylshikimate-3-phosphate synthase genes 1 and 2 were obtained. Results indicate that a majority of glyphosate-resistant accessions from Ontario possessed a proline to serine substitution at position 106, which has previously been reported to confer glyphosate resistance in other crop and weed species. Accessions possessing this substitution demonstrated notably higher levels of resistance than non–target site resistant (NTSR) accessions from within or outside the growing region and were observed to form a subpopulation genetically distinct from geographically proximate glyphosate-susceptible and NTSR accessions. Although it is unclear whether other non–target site resistance mechanisms are contributing to the levels of resistance observed in target-site resistant accessions, these results indicate that, at a minimum, selection for Pro-106-Ser has occurred in addition to selection for non–target site resistance and has significantly enhanced the levels of resistance to glyphosate in C. canadensis accessions from Ontario.

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Corresponding author

Corresponding author’s E-mail: eric.page@agr.gc.ca

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a

Current address of sixth author: Agassiz Research and Development Centre, 6947 Highway 7 Agassiz, BC V0M 1A0, Canada

Associate Editor for this paper: Marie A. Jasieniuk, University of California.

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References

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Abercrombie, LG, et al. (2009) Permanent genetic resources added to molecular ecology resources database 1 January 2009–30 April 2009. Molecular Ecol Resour 9:13751379
Alarcón-Reverte, R, García, A, Urzúa, J, Fischer, AJ (2013) Resistance to glyphosate in junglerice (Echinochloa colona) from California. Weed Sci 61:4854
Alarcón-Reverte, R, García, A, Watson, SB, Abdallah, I, Sabaté, S, Hernández, MJ, Dayan, FE, Fischer, AJ (2015) Concerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California. Pest Manag Sci 71:9961007
Bell, MS, Hager, AG, Tranel, PJ (2013) Multiple resistance to herbicides from four site-of-action groups in waterhemp (Amaranthus tuberculatus). Weed Sci 61:460468
Beres, ZT, Ernst, EE, Snow, AA, Parish, J, Owen, MDK, Ackley, BA, Loux, MM (2015) Screening for resistance to 20X glyphosate in biotypes of Conyza canadensis from soybean fields and non-agricultural habitats in Ohio and Iowa. Weed Science Society of America Annual Meeting. Lexington, KY, February 9–12, 2015
Bostamam, Y, Malone, JM, Dolman, FC, Boutsalis, P, Preston, C (2012) Rigid ryegrass (Lolium rigidum) populations containing a target site mutation in EPSPS and reduced glyphosate translocation are more resistant to glyphosate. Weed Sci 60:474479
Budd, C (2016) Improving the Consistency of Glyphosate-Resistant Canada fleabane (Conyza canadensis) Control with Saflufenacil: Distribution and Control in Soybean (Glycine max). MSc dissertation. Guelph, ON, Canada: University of Guelph. 154 p
Byker, HP, Soltani, N, Robinson, DE, Tardif, FJ, Lawton, MB, Sikkema, PH (2013) Control of glyphosate-resistant Canada fleabane [Conyza canadensis (L.) Cronq.] with preplant herbicide tankmixes in soybean [Glycine max.(L). Merr.]. Can J Plant Sci 93:659667
Caseley, JC, Coupland, D (1985) Environmental and plant factors affecting glyphosate uptake, movement and activity. Pages 92123 in Grossbard E & Atkinson D eds. The Herbicide Glyphosate. London: Butterworth
Cavan, G, Biss, P, Moss, SR (1998) Localized origins of herbicide resistance in Alopecurus myosuroides . Weed Res 38:239245
Chandi, A, Milla-Lewis, SR, Jordan, DL, York, AC, Burton, JD, Zuleta, MC, Whitaker, JR, Culpepper, AS (2013) Use of AFLP markers to assess genetic diversity in Palmer amaranth (Amaranthus palmeri) populations from North Carolina and Georgia. Weed Sci 61:136145
Charlesworth, D (2003) Effects of inbreeding on the genetic diversity of populations. Phil Trans R Soc Lond B 358:10511070
Dauer, JT, Mortensen, DA, VanGessel, MJ (2007) Temporal and spatial dynamics of long-distance Conyza canadensis seed dispersal. J App Ecol 44:105114
de Carvalho, LB, Cruz-Hipolito, H, González-Torralva, F, Alves, PLCA, Christoffoleti, PJ, De Prado, R (2011) Detection of sourgrass (Digitaria insularis) biotypes resistant to glyphosate in Brazil. Weed Sci 59:171176
Denis, M-H, Delrot, S (1993) Carrier-mediated uptake of glyphosate in broad bean (Vicia faba) via a phosphate transporter. Physiol Plant 87:569575
Dinelli, G, Marotti, I, Bonetti, A, Minelli, M, Catizone, P, Barnes, J (2006) Physiological and molecular insight on the mechanisms of resistance to glyphosate in Conyza canadensis (L.) Cronq. biotypes. Pestic Biochem Physiol 86:3041
Feng, PC, Tran, M, Chiu, T, Sammons, RD, Heck, GR, CaJacob, CA (2004) Investigations into glyphosate-resistant horseweed (Conyza canadensis): retention, uptake, translocation, and metabolism. Weed Sci 52:498505
Filiz, E, Koc, I (2016) Genome-wide identification and comparative analysis of EPSPS (aroA) genes in different plant species. J Plant Bioch Biot 25:2129
Gaines, TA, Wright, AA, Molin, WT, Lorentz, L, Riggins, CW, Tranel, PJ, Beffa, R, Westra, P, Powles, SB (2013) Identification of genetic elements associated with EPSPS gene amplification. PloS One 8:e65819
Garg, B, Vaid, N, Tuteja, N (2014) In-silico analysis and expression profiling implicate diverse role of EPSPS family genes in regulating developmental and metabolic processes. BMC Res Notes 7:58
Ge, X, d’Avignon, DA, Ackerman, JJ, Sammons, RD (2010) Rapid vacuolar sequestration: the horseweed glyphosate resistance mechanism. Pest Manag Sci 66:345348
González-Torralva, F, Rojano-Delgado, AM, de Castro, MDL, Mülleder, N, De Prado, R (2012) Two non-target mechanisms are involved in glyphosate-resistant horseweed (Conyza canadensis L. Cronq.) biotypes. J Plant Physiol 169:16731679
Gougler, JA, Geiger, DR (1981) Uptake and distribution of N-(phosphonomethyl) glycine in sugar beet plants. Plant Physiol 68:668672
Hamrick, JL, Godt, MJ (1996) Effects of life history traits on genetic diversity in plant species. Phil Trans R Soc Lond B 351:12911298
Heap, I (2017) The International Survey of Herbicide Resistant Weeds. www.weedscience.org. Accessed: June 29, 2017
Jasieniuk, M, Ahmad, R, Sherwood, AM, Firestone, JL, Perez-Jones, A, Lanini, WT, Mallory-Smith, C, Stednick, Z (2008) Glyphosate-resistant Italian ryegrass (Lolium multiflorum) in California: distribution, response to glyphosate, and molecular evidence for an altered target enzyme. Weed Sci 56:496502
Kaundun, SS, Zelaya, IA, Dale, RP, Lycett, AJ, Carter, P, Sharples, KR, McIndoe, E (2008) Importance of the P106S target-site mutation in conferring resistance to glyphosate in a goosegrass (Eleusine indica) population from the Philippines. Weed Sci 56:637646
Kirkwood, RC, Hetherington, T, Reynolds, TL, Marshall, G (2000) Absorption, localisation, translocation and activity of glyphosate in barnyardgrass (Echinochloa crus-galli (L.) Beauv): influence of herbicide and surfactant concentration. Pest Manag Sci 56:359367
Kumar, S, Stecher, G, Tamura, K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33:18701874
Liu, J, Davis, AS, Tranel, PJ (2012) Pollen biology and dispersal dynamics in waterhemp (Amaranthus tuberculatus). Weed Sci 60:416422
Lu, YQ, Baker, J, Preston, C (2007) The spread of resistance to acetolactate synthase inhibiting herbicides in a wind borne, self-pollinated weed species. Lactuca serriola L. Theor Appl Genet 115:443450
Meirmans, PG (2006) Using the AMOVA framework to estimate a standardized genetic differentiation measure. Evolution 60:23992402
Meirmans, PG (2012) AMOVA-based clustering of accession genetic data. J Hered 103:744750
Meirmans, PG, Hedrick, PW (2011) Assessing population structure: FST and related measures. Molec Ecol Resour 11:518
Menchari, Y, Délye, C, Le Corre, V (2007) Genetic variation and population structure in black-grass (Alopecurus myosuroides Huds.), a successful, herbicide-resistant, annual grass weed of winter cereal fields. Molec Ecol 16:31613172
Moretti, ML, Hanson, BD (2017) Reduced translocation is involved in resistance to glyphosate and paraquat in Conyza bonariensis and Conyza canadensis from California. Weed Res 57:2534
Nandula, VK, Ray, JD, Ribeiro, DN, Pan, Z, Reddy, KN (2013) Glyphosate resistance in tall waterhemp (Amaranthus tuberculatus) from Mississippi is due to both altered target-site and nontarget-site mechanisms. Weed Sci 61:374383
Nandula, VK, Reddy, KN, Poston, DH, Rimando, AM, Duke, SO (2008) Glyphosate tolerance mechanism in Italian ryegrass (Lolium multiflorum) from Mississippi. Weed Sci. 56:344349
Okada, M, Hanson, BD, Hembree, KJ, Peng, Y, Shrestha, A, Stewart, CN, Wright, SD, Jasieniuk, M (2013) Evolution and spread of glyphosate resistance in Conyza canadensis in California. Evol Appl 6:761777
Peakall, R, Smouse, PE (2012) GenAlEx 6.5: genetic analysis in Excel. Accession genetic software for teaching and research—an update. Bioinformatics 28:25372539
Peng, Y, Abercrombie, LL, Yuan, J S, Riggins, CW, Sammons, RD, Tranel, PJ, Stewart, CN (2010) Characterization of the horseweed (Conyza canadensis) transcriptome using GS-FLX 454 pyrosequencing and its application for expression analysis of candidate non-target herbicide resistance genes. Pest Manag Sci 66:10531062
Peng, Y, Lai, Z, Lane, T, Nageswara-Rao, M, Okada, M, Jasieniuk, M, O’Geen, H, Kim, RW, Sammons, RD, Rieseberg, LH, Stewart, CN (2014) De novo genome assembly of the economically important weed horseweed using integrated data from multiple sequencing platforms. Plant Physiol 166:12411254
Perez-Jones, A, Park, KW, Polge, N, Colquhoun, J, Mallory-Smith, CA (2007) Investigating the mechanisms of glyphosate resistance in Lolium multiflorum . Planta 226:395404
Preston, C, Wakelin, AM, Dolman, FC, Bostamam, Y, Boutsalis, P (2009) A decade of glyphosate-resistant Lolium around the world: mechanisms, genes, fitness, and agronomic management. Weed Sci 57:435441
Riar, DS, Rustgi, S, Burke, IC, Gill, KS, Yenish, JP (2010) EST-SSR development from 5 Lactuca species and their use in studying genetic diversity among L. serriola biotypes. J Hered 102:1728
Sammons, RD, Gaines, TA (2014) Glyphosate resistance: state of knowledge. Pest Manag Sci 70:13671377
Schuelke, M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233234
Seefeldt, SS, Jensen, JE, Fuerst, EP (1995) Log-logistic analysis of herbicide dose-response relationships. Weed Technol 1:218227
Shields, EJ, Dauer, JT, VanGessel, MJ, Neumann, G (2006) Horseweed (Conyza canadensis) seed collected in the planetary boundary layer. Weed Sci 54:10631067
Siehl, DL (1997) Inhibitors of EPSP synthase, glutamine synthase and histidine synthesis. Pages 3767 in Roe RM, Burton JD & Kuhr RJ eds., Herbicide Activity: Toxicology, Biochemistry and Molecular Biology. Amsterdam: IOS Press
Smisek, A (1995) The Evolution of Resistance to Paraquat in Populations of Erigeron canadensis L. MSc dissertation. London, ON, Canada: University of Western Ontario. 103 p
Smisek, A, Doucet, C, Jones, M, Weaver, S (1998) Paraquat resistance in horseweed (Conyza canadensis) and Virginia pepperweed (Lepidium virginicum) from Essex County, Ontario. Weed Sci 46:200204
Steinrucken, HC, Amrhein, N (1980) The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase. Biochem Biophys Res Comm 94:12071212
Tsuji, R, Fischer, AJ, Yoshino, M, Roel, A, Hill, JE, Yamasue, Y (2003) Herbicide-resistant late watergrass (Echinochloa phyllopogon): similarity in morphological and amplified fragment length polymorphism traits. Weed Sci 51:740747
VanGessel, MJ (2001) Glyphosate-resistant horseweed from Delaware. Weed Sci 49:703705
Vink, JP, Soltani, N, Robinson, DE, Tardif, FJ, Lawton, MB, Sikkema, PH (2012) Occurrence and distribution of glyphosate-resistant giant ragweed (Ambrosia trifida L.) in southwestern Ontario. Can J Plant Sci 92:533539
Wakelin, AM, Preston, C (2006) A target-site mutation is present in a glyphosate-resistant Lolium rigidum population. Weed Res 46:432440
Weaver, S, Downs, M, Neufeld, B (2004) Response of paraquat-resistant and-susceptible horseweed (Conyza canadensis) to diquat, linuron, and oxyfluorfen. Weed Sci 52:549553
Weaver, SE (2001) The biology of Canadian weeds. 115. Conyza canadensis. Can J Plant Sci 81:867875
Wright, S (1965) The interpretation of accession structure by F-statistics with special regard to systems of mating. Evolution 19:395420
Yu, Q, Jalaludin, A, Han, H, Chen, M, Sammons, RD, Powles, SB (2015) Evolution of a double amino acid substitution in the EPSP synthase in Eleusine indica conferring high level glyphosate resistance. Plant Physiol 167:14401447
Yuan, JS, Abercrombie, LL, Cao, Y, Halfhill, M D, Zhou, X, Peng, Y, Hu, J, Rao, MR, Heck, GR, Larosa, TJ, Sammons, RD, Wang, X, Ranjan, P, Johnson, DH, Wadl, PA, Scheffler, BE, Rinehart, TA, Trigiano, RN, Stewart, CN (2010) Functional genomics analysis of horseweed (Conyza canadensis) with special reference to the evolution of non–target-site glyphosate resistance. Weed Sci 58:109117

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