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Site of Action of Oxyfluorfen

  • M. K. Pritchard (a1), G. F. Warren (a1) and R. A. Dilley (a2)


The effects of oxyfluorfen [2-chloro-1-(3-ethoxy-4-nitrophenoxy)-4-(trifluoromethyl)benzene] were studied on electron transport and phosphorylation in isolated spinach (Spinacia oleracea L.) chloroplasts and on the response of green bean (Phaseolus vulgaris L. ‘Spartan Arrow’) to applications of oxyfluorfen alone or in combination with other herbicides. Coupled non-cyclic electron transfer and phosphorylation through photosystems I and II (H2 O å methyl viologen) were inhibited about 30% and 55%, respectively, by 10−4 M oxyfluorfen. Photosystem II-linked phosphorylation with dimethylbenzoquinone (2,5-dimethyl-p-benzoquinone) as the electron acceptor (H2 O å DMQ) was completely inhibited by 10−4 M oxyfluorfen. Photosystem II electron transport with dimethylbenzoquinone as the electron acceptor was inhibited 60% by 10−4 M oxyfluorfen, whereas photosystem I electron transport with 2,6-dichlorophenol indophenol as electron donor (DCIPH2 å MV) was not susceptible to oxyfluorfen inhibition. Photosystem I-linked phosphorylation and the accompanying electron transport supported by durohydroquinone electron donation (DQH2 å MV) were inhibited about 50% by 10−4 M oxyfluorfen, whereas cyclic phosphorylation was not inhibited at that concentration. Increased conductivity of a solution that contained leaf discs taken from green beans treated with various combinations of foliar-applied herbicides was a measure of membrane damage caused by the herbicides, and revealed that oxyfluorfen has a different site of action than do photosynthesis inhibitor and bipyridilium herbicides. Oxyfluorfen plus dinoseb (2-sec-butyl-4,6-dinitrophenol) injury to green beans was additive, but the two herbicides did not have the same site of action. Oxyfluorfen did not appear to inhibit electron transport in chloroplasts at herbicidal rates, nor was it dependent on electron transport for activation.



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1. Ahrens, J. F. 1979. Repeated applications of granular herbicides on container-grown ornamentals. Abstr. Weed Sci. Soc. Am. p. 53.
2. Arnon, D. I. 1949. Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris . Plant Physiol. 24:115.
3. Ashton, F. M. and Crafts, A. S. 1973. Mode of Action of Herbicides. Wiley, New York, 504 pp.
4. Ashton, F. M., Gifford, E. M. Jr., and Bisalputra, T. 1963. Structural changes in Phaseolus vulgaris induced by atrazine. I. Histological changes. Bot. Gaz. 124:329335.
5. Avron, M. 1975. The electron transport chain in chloroplasts. Pages 373386 in Govindjee, , ed. Bioenergetics of Photosynthesis. Academic Press, New York.
6. Berg, S. P. and Izawa, S. 1977. Pathways of silicomolybdate photoreduction and associated photophosphorylation in tobacco chloroplasts. Biochim. Biophys. Acta 460:206219.
7. Bugg, M. W., Whitmarsh, J., Rieck, C. E., and Cohen, W. S. 1980. Inhibition of photosynthetic electron transport by diphenyl ether herbicides. Plant Physiol. 65:4750.
8. Colby, S. R. 1967. Calculating synergistic and antagonistic responses of herbicide combinations. Weeds 15:2022.
9. Fadayomi, O. and Warren, G. F. 1976. The light requirement for herbicide activity of diphenylethers. Weed Sci. 24:598600.
10. Farrington, J. A., Ebert, M., Land, E. J., and Fletcher, K. 1973. Bipyridilium quaternary salts and related compounds. V. Pulse hydrolysis studies of the reaction of paraquat radical with oxygen. Implications for the mode of action of bipyridyl herbicides. Biochim. Biophys. Acta 314:372381.
11. Fork, D. C. 1972. Oxygen electrode. Pages 113122 in San Peitro, A., ed. Methods Enzymol. Vol. 24, Academic Press, New York.
12. Izawa, S. and Good, N. E. 1972. Inhibitors of photosynthetic electron transport and photophosphorylation. Pages 355377 in San Pietro, A., ed. Methods Enzymol. Vol. 24. Academic Press, New York.
13. Izawa, S., Gould, J. M., Ort, D. R., Felker, P., and Good, N. E. 1973. Electron transport and photophosphorylation in chloroplasts as a function of the electron acceptor. III. A dibromothymoquinone-insensitive phosphorylation reaction associated with photosystem II. Biochim. Biophys. Acta 305:119128.
14. Izawa, S. and Pan, R. L. 1978. Photosystem I electron transport and phosphorylation supported by electron donation to the plastoquinone region. Biochem. Biophys. Res. Commun. 83: 11711177.
15. Jagendorf, A. T. and Hind, G. 1963. Studies on the mechanism of photophosphorylation. Pages 599610, in Photosynthetic Mechanisms of Green Plants. N.A.S.-N.R.C. Publ. No. 1145.
16. Jordan, T. N. and Warren, G. F. 1975. Effects of prometryn and dinoseb combinations in an undiluted oil carrier. Weed Sci. 23: 328332.
17. Kapusta, G. and Streiker, C. F. 1976. New soybean preemergence herbicide study, 1976. Res. Rep. North Cent. Weed Control Conf. p. 277.
18. Matsunaka, S. 1969. Acceptor of light energy in photoactivation of diphenylether herbicides. J. Agric. Food Chem. 17:171174.
19. Matsunaka, S. 1969. Activation and inactivation of herbicides by higher plants. Residue Rev. 25:4558.
20. Mees, G. C. 1960. Experiments on the herbicidal action of 1,1′-ethylene-2,2′-dipyridinium dibromide. Ann. Appl. Biol. 48:601612.
21. Moreland, D. E., Blackman, W. J., Todd, J. G., and Farmer, F. S. 1970. Effects of diphenylether herbicides on reactions of mitochondria and chloroplasts. Weed Sci. 18:636642.
22. Neumann, J., Arntzen, C. J., and Dilley, R. A. 1971. Two sites for adenosine triphosphate formation in photosynthetic electron transport mediated by photosystem I. Evidence from digitonin subchloroplast particles. Biochemistry 10:866873.
23. Neumann, J. and Jagendorf, A. T. 1964. Light induced pH change related to phosphorylation by chloroplasts. Arch. Biochem. Biophys. 107:109119.
24. Ort, D. R. and Izawa, S. 1973. Studies on the energy – coupling sites of photophosphorylation. II. Treatment of chloroplasts with NH2OH plus ethylenediaminotetra-acetate to inhibit water oxidation while maintaining energy-coupling efficiencies. Plant Physiol. 52:595600.
25. Prendeville, G. N. and Warren, G. F. 1977. Effect of herbicides on leaf-cell membrane permeability. Weed Res. 30:251258.
26. Vanstone, D. E. and Stobbe, E. H. 1977. Electrolytic conductivity – a rapid measure of herbicide injury. Weed Sci. 25:352354.
27. Vanstone, D. E. and Stobbe, E. H. 1979. Light requirement of the diphenylether herbicide oxyfluorfen. Weed Sci. 27:8891.
28. White, G. C., Chain, R. K., and Malkin, R. 1978. Duroquinol as an electron donor for chloroplast electron transfer reactions. Biochim. Biophys. Acta 502:127137.
29. Wojtaszek, T. 1966. Relationship between susceptibility of plants to DNBP and their capacity for ATP generation. Weeds 14:125129.


Site of Action of Oxyfluorfen

  • M. K. Pritchard (a1), G. F. Warren (a1) and R. A. Dilley (a2)


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