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Resistance Profile of Diclofop-Resistant Italian Ryegrass (Lolium multiflorum) to ACCase- and ALS-Inhibiting Herbicides in Arkansas, USA

  • Yong In Kuk (a1), Nilda R. Burgos (a2) and Robert C. Scott (a3)


Diclofop-resistant Italian ryegrass is a major weed problem in wheat production. This study aimed to determine the resistance pattern of diclofop-resistant Italian ryegrass accessions from the southern United States to the latest commercialized herbicides for wheat production, pinoxaden and mesosulfuron, and to other acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibitors. Twenty-nine of 36 accessions were resistant to the commercial dose of diclofop. The majority (80%) of diclofop-resistant accessions were also resistant to clodinafop. Of 25 diclofop-resistant accessions, 5 were resistant to pinoxaden. All accessions tested were susceptible to the commercial dose of clethodim and sethoxydim. The cross-resistance pattern of diclofop-resistant Italian ryegrass to other ACCase inhibitors was 20% for pinoxaden and none with clethodim or sethoxydim. One accession was resistant to mesosulfuron but not to diclofop. This mesosulfuron-resistant accession was cross-resistant to sulfometuron but not to imazamox. All diclofop-resistant accessions tested were susceptible to ALS inhibitors, mesosulfuron, sulfometuron, and imazamox. Therefore, diclofop-resistant Italian ryegrass in Arkansas can be controlled with imazamox (in Clearfield wheat) and can mostly be controlled with mesosulfuron and pinoxaden. It could also be controlled by other selective grass herbicides in broadleaf crops.


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Anonymous 2004. Finesse Herbicide Supplemental Labeling: Rotational Interval to STS Soybean, Grain Sorghum, Cotton, Non-STS Soybeans, Field Corn, and Rice. Accessed: December 26, 2007.
Bailey, W.A., Wilson, H.P., and Hines, T.E. 2003a. Influence of AE F130060 03 application timing on Italian ryegrass (Lolium multiflorum) control. Weed Technol. 17:842853.
Bailey, W.A., Wilson, H.P., and Hines, T.E. 2003b. Response of winter wheat and diclofop-methyl-sensitive and resistant Italian ryegrass (Lolium multiflorum) to AE F130060 03. Weed Sci. 51:515522.
Bond, J.A., Stephenson, D.O. IV, Barnes, J.W., Bararpour, M.T., and Oliver, L.R. 2005. Diclofop-resistant Italian ryegrass (Lolium multiflorum) control in imidazolinone-tolerant wheat. Weed Technol. 19:437442.
Bravin, F., Zanin, G., and Preston, C. 2001. Diclofop-methyl resistance in populations of Lolium spp. from central Italy. Weed Res. 41:4958.
Clemmer, K.C., York, A.C., and Brownie, C. 2004. Italian ryegrass (Lolium multiflorum) control in imidazolinone-resistant wheat. Weed Technol. 8:481489.
Cocker, K.M., Northcroft, D.S., Coleman, J.O.D., and MOSS, S.R. 2001. Resistance to ACCase-inhibiting herbicides and isoproturon in UK biotypes of Lolium multiflorum: mechanisms of resistance and implications for control. Pest Manag. Sci. 57:587597.
Crooks, H.L., York, A.C., and Jordan, D.L. 2004. Wheat (Triticum aestivum) tolerance and Italian ryegrass (Lolium multiflorum) control with AE F130060 00 plus AE F115008 00 applied in nitrogen. Weed Technol. 18:9399.
Delye, C., Zhang, X-Q., Chalopin, C., Michael, S., and Powles, S.B. 2003. An isoleucine residue within the carboxyl-transferase domain of multidomain acetyl-CoA carboxylase is a major determinant of sensitivity to aryloxyphenoxypropionate, but not to cyclohexanedione inhibitors. Plant Physiol. 132:17161723.
Eleni, K.S., Tal, A., and Rubin, B. 2000. Diclofop-resistant Lolium rigidum from northern Greece with cross-resistance to ACCase inhibitors and multiple resistance to chlorsulfuron. Pest Manag. Sci. 56:10541058.
Friesen, L.F., Morrison, I.N., Rashid, A., and Devine, M.D. 1993. Response of a chlorsulfuron-resistant biotype of Kochia scoparia to sulfonylurea and alternative herbicides. Weed Sci. 41:100106.
Grey, T.L. and Bridges, D.C. 2003. Alternatives to diclofop for the control of Italian ryegrass (Lolium multiflorum) in winter wheat (Triticum aestivum). Weed Technol. 17:219223.
Hall, L.M. and Devine, M.D. 1990. Cross-resistance of chlorsulfuron-resistant biotype of Stellaria media to a triazolopyrimidine herbicide. Plant Physiol. 93:962966.
Hand, S.S., Smith, T.L., Sanderson, J., Barr, G., Strachan, W.F., and Paulsgrove, M. 2002. AE F130060 00- a new selective herbicide for grass control in wheat. Proc. South. Weed Sci. Soc. 55:142143.
Heap, I.M. 2007. International Survey of Herbicide-Resistant Weeds.
Heap, I.M. and Knight, R. 1982. A population of ryegrass tolerant to the herbicide diclofop-methyl. J. Aust. Inst. Agric. Sci. 48:1156–157.
Holtum, J.A.M. and Powles, S.B. 1991. Annual ryegrass: an abundance of resistance, a plethora of mechanisms. Pages 10711077. in. Proceedings of Brighton Crop Protection Conference. Farnham, Surrey, UK Weeds, BCPC.
Kuk, Y.I. and Burgos, N.R. 2007. Cross-resistance profile of mesosulfuron-resistant Italian ryegrass in the southern USA. Pest Manag. Sci. 63:349357.
Kuk, Y.I., Burgos, N.R., and Talbert, R.E. 2000. Cross- and multiple resistance of diclofop-resistant Lolium spp. Weed Sci. 48:412419.
Lovell, S.T., Wax, L.M., Horak, M.J., and Peterson, D.E. 1996. Imidazolinone and sulfonylurea resistance in a biotype of common waterhemp (Amaranthus rudis). Weed Sci. 44:789794.
Mallory-Smith, C.A., Thill, D.C., and Dial, M.J. 1990. Identification of sulfonylurea herbicide-resistant prickly lettuce (Lactuca serriola). Weed Technol. 4:163168.
Nicholskaya, T., Zagnitko, O., Tevzadze, G., Haselkorn, R., and Gornicki, P. 1999. Herbicide sensitivity determinant of wheat plastid acetyl-CoA carboxylase is located in a 400-amino acid fragment of the carboxyltransferase domain. Proc. Natl. Acad. Sci. U. S. A. 96:1464714651.
Porter, D.J., Kopec, M., and Hofer, U. 2005. Pinoxaden—a new selective postemergence graminicide for wheat and barley. Weed Sci. Soc. Am. 45:95. [Abstract].
Primiani, M.M., Cotterman, C., and Saari, L.L. 1990. Resistance of kochia (Kochia scoparia) to sulfonylurea and imidazolinone herbicides. Weed Technol. 4:169172.
Ritter, R.L. and Menbere, H. 2002. Preemergence control of Italian ryegrass (Lolium multiflorum) in wheat (Triticum aestivum). Weed Technol. 16:5559.
Saari, L.L., Cotterman, C., Smith, W.F., and Primiani, M.M. 1992. Sulfonylurea herbicide resistance in common chickweed, perennial ryegrass, and Russian thistle. Pestic. Biochem. Physiol. 42:110118.
Saari, L.L., Cotterman, J.C., and Thill, D.C. 1994. Resistance to acetolactate synthase inhibiting herbicides. Pages 83139. in Powles, S.B. and Holtum, J.A.M. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL Lewis.
Tal, A. and Rubin, B. 2004. Molecular characterization of resistance to ACCase-inhibiting herbicides in Lolium rigidum . Pest Manag. Sci. 60:10131018.
Tardif, F.J. and Powles, S.B. 1994. Herbicide multiple-resistance in Lolium rigidum biotypes is endowed by multiple mechanisms: isolation of a subset with resistant acetylcoenzyme A carboxylase. Physiol. Planta. 91:488494.
Vencill, W.K. 2002. Herbicide Handbook. 8th ed. Champaign, IL Weed Science Society of America. 493.


Resistance Profile of Diclofop-Resistant Italian Ryegrass (Lolium multiflorum) to ACCase- and ALS-Inhibiting Herbicides in Arkansas, USA

  • Yong In Kuk (a1), Nilda R. Burgos (a2) and Robert C. Scott (a3)


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