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Method for Bioassaying Chlorsulfuron in Soil and Water

  • Don W. Morishita (a1), Donald C. Thill (a1), Duane G. Flom (a1), Tanaquil C. Campbell (a1) and Gary A. Lee (a1)...

Abstract

Bioassay techniques were evaluated for the determination of chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino] carbonyl] benzenesulfonamide} residues in soil and water. A linear response of corn (Zea mays L.) primary root length to chlorsulfuron concentrations (InX+1) of 0.0 to 16.0 μg/L was observed in the water bioassay. Several soil extraction bioassay methods were attempted and found to be highly variable. A Ca(OH)2 soil extraction bioassay technique produced a linear response of primary corn root length to chlorsulfuron concentrations ranging from 0.0 to 64.0 μg/kg. The efficiency of the Ca(OH)2 extraction bioassay was determined by the use of 14C-chlorsulfuron. Recovery efficiency at 1.0 μg/kg was 74% and averaged 62% at all higher chlorsulfuron concentrations.

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