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Identification and characterization of different accessions of itchgrass (Rottboellia cochinchinensis)

  • Pedro Luís C. A. Alves, Mateus F. Bachega (a1), José Roberto Moro (a1), Manoel Victor F. Lemos (a1), Eliane C. C. Alves (a1), Marco Aurélio S. Silva (a1) and Fabíola V. Moro (a1)...

Abstract

Studies were conducted to identify and characterize different accessions of itchgrass. Seeds were collected in the counties of Aramina, Campinas, Dumont, Igarapava, Jaboticabal, and Ribeirão Preto, all in the state of São Paulo, Brazil. Accessions were characterized based on dimensions of their stomata, stomatal index (SI), and length and width of their seed (caryopses and husk). Chromosome number and length also were determined, and accessions were further differentiated using molecular markers (polymerase chain reaction [PCR]). Itchgrass from Ribeirão Preto had much longer and narrower seeds than those from the other locations, and their husks were longer as well. Accessions had similar SIs, both on the abaxial and adaxial leaf surfaces. Stomata from Campinas and Igarapava accessions were longer and wider, whereas those from Dumont and Ribeirão Preto were similar and smaller than all others. The accession from Ribeirão Preto is diploid (2n = 20); the rest are polyploid, with the total length of chromosomes smaller than all others. These differences were confirmed by molecular differentiation (PCR).

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Corresponding author

Corresponding author. DBAA–FCAV/UNESP, Rod. de Acesso Prof. Paulo Donato Castellane, s/n CEP 14884-900, Jaboticabal, SP, Brazil; plalves@fcav.unesp.br

References

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Arévalo, R. A. and Bertoncini, E. I. 1994. Biologia e manejo de Rottboellia exalta L. f. na cultura da cana-de-açúcar Saccharum spp: análise do problema. Piracicaba, Brazil: Estação Experimental de cana-de-açècar—IAC, 1992. 24 p. [Publicação especial, 2]
Brasileiro, A.C.M. and Carneiro, V.T.C. 1998. Manual de transormação genética de plantas. Brasília, Brazil: EMBRAPA-SPI. 309 p.
Cepero, G. and Rodriguez, G. S. 1983. Weeds in sugarcane (Saccharum sp.) hybrids. Cent. Agric. 10:4755.
Cristopher, J., Kumari Sasikala, K. S., and Mini, L. S. 1989. Cyto-taxonomic studies of Rottboellia exaltata Linn. complex. Cytologia 54:335342.
Cutter, E. G. 1978. Plant Anatomy. Part I. Cells and Tissues. London: Edward Arnold. 313 p.
Ferreira, M. E. and Grattapaglia, D. 1995. Introdução ao uso de marcadores moleculares em análise genética. 2nd ed. Brasília, Brazil: EMBRAPA-CENARGEN. pp. 1112.
Fisher, H. H., Menendez, R. A., Daley, L. S., Robb-Spencer, D., and Crabtree, G. D. 1987. Biochemical characterization of itchgrass (Rottboellia exaltata) biotypes. Weed Sci. 35:333338.
Holm, L. G., Plucknett, D. L., Pancho, J. V., and Herberger, J. P. 1977. The World's Worst Weeds. Distribution and Biology. Honolulu: University Press of Hawaii. pp. 139144.
Kissmann, K. G. 1997. Plantas infestantes e nocivas. Tomo-I. 2nd ed. São Paulo, Brazil: Basf Brasileira S.A. pp. 723727.
Mercado, B. L. 1978. Biology, problems and control of Rottboellia exaltata L. f., a monograph. Biotrop. Bull. Volume 14, 38 p.
Millhollon, R. W. 1982. Reproduction variability in biotypes of johnsongrass (Sorghum halepense (L.) Pers.) and itchgrass (Rottboellia exaltata L. f.). Weed Sci. 22:2223.
Millhollon, R. W. 1992. Effects of itchgrass (Rottboellia cochinchinensis) interference in growth and yield of sugarcane (Saccharum sp.) hybrids. Weed Sci. 40:4853.
Millhollon, R. W. and Burner, D. M. 1993. Itchgrass (Rottboellia exaltata) biotypes in world populations. Weed Sci. 41:379387.
Morales, R. and Fernandes, F. 1985. Economic damage threshold for Rottboellia exaltata in sugarcane. Cien. Tec. Agric. Prot. Plantas 8:5566.
Pamplona, P. P. and Mercado, B. H. 1981. Ecotypes of Rottboellia exaltata L. f. in the Philippines. II. Response to daylength and nitrogen application. Philipp. Agric. 64:371378.
Silva, C.E.B., Pavani, M.C.M.D., and Alves, P. L.C.A. 1996. Fatores que afetam a germinação de sementes de Rottboellia exaltata: I. Temperatura e disponibilidade de água. In Proceedings of the 9th Congresso da Sociedade Botânica de São Paulo; São Carlos, SP, Brazil Sociedade Botânica de São Paulo. p. 78.
Singh, R. J. 1993. Plant Cytogenetics. Tokyo: CRC Press. pp. 569574.
Williams, J.G.K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., and Tingey, S. V. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:65316535.

Keywords

Identification and characterization of different accessions of itchgrass (Rottboellia cochinchinensis)

  • Pedro Luís C. A. Alves, Mateus F. Bachega (a1), José Roberto Moro (a1), Manoel Victor F. Lemos (a1), Eliane C. C. Alves (a1), Marco Aurélio S. Silva (a1) and Fabíola V. Moro (a1)...

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