Skip to main content Accessibility help
×
Home

Evidence of High Gene Flow Between Samples of Horseweed (Conyza canadensis) and Hairy Fleabane (Conyza bonariensis) as Revealed by Isozyme Polymorphisms

  • Alaim Anderson Fernandes Soares (a1), Angela Maria Dalla Torre Fregonezi (a1), Denis Bassi (a1), Claudete Aparecida Mangolin (a1), Sandra Aparecida de Oliviera Collet (a1), Rubem Silvério de Oliveira Junior (a2) and Maria de Fátima Pires da Silva Machado (a1)...

Abstract

Native polyacrylamide gel electrophoresis was used to identify polymorphisms in α- and β-esterases loci and electrophoresis in starch gel to identify polymorphism in malate dehydrogenase (MDH; EC 1.1.1.37) and acid phosphatase (ACP; EC 3.1.3.2) isozymes loci in leaf tissues from samples of horseweed and hairy fleabane populations to determine genetic diversity and population structure. Similar or differential genetic divergence between the two species may guide specific use of herbicides. For samples of plants with high genetic similarity it is possible to adopt similar mechanisms and processes for their control. The proportion of polymorphic loci was 57.14, 50.0, and 53.6%, in samples of horseweed and hairy fleabane, for EST, MDH, and ACP isozymes, respectively. A comparison of the diversity parameters in the two species showed that the number of alleles is similar in the horseweed and hairy fleabane plants. The estimated heterozygosity in horseweed and hairy fleabane was also very close. A relatively low level of population differentiation was detected between horseweed and hairy fleabane (FST = 0.0199), which suggests a substantial genetic exchange among the two species. Accordingly, estimate of gene flow was high (Nm = 12.3172) for the alleles of the loci Est, Mdh, and Acp. The Nei's identity (I) values also was high (I = 0.9561) indicating very high similarity between the two Conyza species. AMOVA showed higher genetic variation within (95%) than among (5%) the two samples. The low genetic structure and high value of genetic identity was an important indication that alleles are exchanged between horseweed and hairy fleabane populations, and provides additional evidence of occurrence of outcrossing between populations or dispersion of samples of one for other site.

Copyright

Corresponding author

Corresponding author's E-mail: mfpsmachado@uem.br

References

Hide All
Allendorf, FW, Luikart, G (2007) Conservation and the genetics of populations. Blackwell Publishing, Malden. 642 p
Carvalho, VM, Marochio, CA, Mangolin, CA, Machado, MFPS (2011) The use of esterase polymorphism for analysis of the genetic diversity and structure of stevia (Stevia rebaudiana Bert. Bertoni) populations. Biochem Syst Ecol. 39:594599
Carvalho, VM, Marques, RM, Lapenta, AS, Machado, MFPS (2003) Functional classification of esterases from leaves of Aspidosperma polyneuron M. Arg. (Apocynaceae). Genet Mol Biol. 26:195198
Ferrer, MM, Good-Avila, SV (2007) Macrophylogenetic analyses of the gain and loss of self-incompatibility in the Asteraceae. New Phytologist. 173:401414
Frankton, C, Mulligan, GA (1987) Weeds of Canada (revised). Publication 948. Ministry of Supply and Services Canada. NC Press Limited, Toronto, ON Frankton and Mulligan. 217 p
Frigo, MJ, Mangolin, CA, Oliveira, RS Jr., Machado, MFPS (2009) Esterase polymorphism for analysis of genetic diversity and structure of wild poinsettia (Euphorbia heterophylla) populations. Weed Sci. 57:5460
González-Torralva, F, Cruz-Hipolito, H, Bastida, F, Mülleder, N, Smeda, RJ, Prado, R (2010) Differential susceptibility to glyphosate among the Conyza weed species in Spain. J Agric Food Chem. 58:43614366
Govindaraju, DR (1989) Variation in gene flow levels among predominantly self-pollinated plants. J Evol Biol. 2:173181
Guareschi, A, Pastori, T, Kruse, ND, Machado, SLO, Dorow, TSC, Tedesco, SB (2012) Cytogenetics characterization of Conyza bonariensis (L.) Cronquist populations from Brazil. Ciência e Natura. 34:3948
Heap I. The International Survey of Herbicide Resistant Weeds. http://www.weedscience.org. Accessed September 8, 2014
Jorge, IC, Mangolin, CA, Machado, MFPS (1997) Malate dehydrogenase isozymes (MDH; EC 1.1.1.37) in long-term callus culture of Cereus peruvianus (Cactaceae) exposed to sugar and temperature stress. Biochem Genet. 35:155164
Kissmann, KG, Groth, D (1999) Plantas Infestantes e Nocivas. 2th ed. São Bernardo do Campo Basf. Pp 152156, 278–284
Lamego, FP, Vidal, RA (2008) Resistência ao glyphosate em biótipos de Conyza bonariensis e Conyza canadensis no Estado do Rio Grande do Sul, Brasil. Planta Daninha. 26:467471
Lazaroto, CA, Fleck, NG, Vidal, RA (2008) Biologia e ecofisiologia de buva (Conyza bonariensis e Conyza canadensis). Ciência Rural. 38:852860
Lopes, R, Bruckner, CH, Lopes, MTG (2002) Polimorfismo isozimático e potencial de utilização das isozimas como marcadores genéticos em aceroleira. Pesq Agropec Brasil. 37:151158
Machado, MFPS, Prioli, AJ, Mangolin, CA (1993) Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in tissue and callus cultures of Cereus peruvianus (Cactaceae). Biochem Genet. 31:167172
Main, CL, Mueller, TC, Hayes, RM, Wilkerson, JB (2004) Response of selected Horseweed (Conyza canadensis (L.) Cronq.) populations to glyphosate. J Agric Food Chem. 52:879883
Mangolin, CA, Prioli, AJ, Machado, MFPS (1997) Isozyme variability in plants regenerated from calli of Cereus peruvianus (Cactaceae). Biochem Genet. 35:189204
McClintock, D, Marshall, JB (1988) On Conyza sumatrensis (Retz) E. Walker and certain hybrids in the genus. Watsonia. 17:172173
Mendes, S, Portugal, J, Calha, I (2012) Glyphosate resistance survey on Conyza canadensis . Revista de Ciências Agrárias. 35:322328
Moreira, MS, Melo, MSC, Carvalho, SJO, Nicolai, M, Christoffoleti, PJ (2010) Herbicidas alternativos para controle de biótipos de Conyza bonariensis e C. canadensis resistentes ao glyphosate. Planta Daninha. 28:167175
Moreira, MS, Nicolai, M, Carvalho, SJP, Christoffoleti, PJ (2007) Glyphosate-resistance in Conyza canadensis and C. bonariensis . Planta Daninha. 25:157164
Mulligan, GA, Findlay, JN (1970) Reproductive systems and colonization in Canadian weeds. Can J Bot. 5:859860
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proc Nat Acad Sci USA. 70:33213323
Newton, KJ (1983) Genetics of mitochondrial isozymes. Pages 159176 in Tanksley, SD, Orton, TJ, eds. Isozymes in Plant Genetics and Breeding, Part A. New York Elsevier Science
Okada, M, Hanson, BD, Hembree, KJ, Peng, Y, Shrestha, A, Stewart, CN Jr., Wright, SD, Jasieniuk, M (2013) Evolution and spread of glyphosate resistance in Conyza canadensis in California. Evolutionary Applications. 6:761777
Oliveira Neto, AM, Constantin, J, Oliviera, RS Jr., Guerra, N, Dan, HA, Alonso, DG, Blainski, E, Santos, G (2010) Winter and summer management strategies for Conyza bonariensis and Bidens pilosa control. Planta Daninha. 28:11071116
Orasmo, GR, Collet, SAO, Lapenta, AS, Machado, MFPS (2007) Biochemical and genetic polymorphisms for carboxylesterase and acetylesterase in grape clones of Vitis vinifera L. (Vitaceae) cultivars. Biochem Genet. 45:663670
Paula, JM, Vargas, L, Agostinetto, D, Nohatto, MA (2011) Management of glyphosate-resistant Conyza bonariensis . Planta Daninha. 29:217227
Peakall, R, Smouse, PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes. 6:288295
Penha, ETS, Aparecido, LEO, Souza, PS, Souza, CAS (2012) Aplicação de doses do herbicida glyphosate no controle da Conyza bonariensis . Rev Agrogeoambiental. 4:16
Pereira, AJ, Lapenta, AS, Vidigal-Filho, PS, Machado, MFPS (2001) Differential esterase expression in leaves of Manihot esculenta Crantz infected with Xanthomonas axonopodis pv. Manihotis. Biochem Genet. 39:289296
Pereira, LK, Mangolin, CA, Scapim, CA, Pacheco, CAP, Bonato, CM, Machado, MFPS (2006) Malate dehydrogenase isozyme patterns in four cycles of half-sib selection from CMS-43 popcorn variety (Zea mays L.). Maydica. 51:561566
Prado, R (1987) Group C1/5 resistant hair fleabane (Conyza bonariensis) . The international survey of herbicide resistant weeds. http://www.weedscience.org. Accessed February 20, 2014
Resende, AG, Vidigal-Filho, PS, Machado, MFPS (2000) Isozyme diversity in cassava cultivars (Manihot esculenta Crantz). Biochem Genet. 38:203211
Sala, J, Mangolin, CA, Franzoni, J, Machado, MFPS (2011) Esterase polymorphism and the analysis of genetic diversity and structure in cactus populations descended from Cereus peruvianus plants regenerated in vitro. Biochem Genet. 49:270282
Smisek, AJ (1995) The evolution of resistance to paraquat in populations of Erigeron canadensis L. . London, Ontario University of Western Ontario. 102 p
Thebaud, C, Abbott, RJ (1995) Characterization of invasive Conyza species (Asteraceae) in Europe: quantitative trait and isozyme analysis. Am J Bot. 3:360368
Thorpe, JP, Solé-Cava, AM (1994) The use of allozyme electrophoresis in invertebrate systematic. Zoologica Scripta. 23:318
Trezzi, MM, Vidal, RA, Xavier, E, Rosin, D, Balbinot, AA, Prates, MA (2011) Resistance to glyphosate in Conyza spp. biotypes in Western and Southwestern Parana, Brazil. Planta Daninha. 29:11131120
Urdampilleta, JD, Amat, AG, Bidau, C (2005) Karyotypic studies and morphological analysis of reproductive features in five species of Conyza (Astereae: Asteraceae) from Northwestern Argentina. Bol Soc Argent Bot. 40:9199
Vargas, L, Bianchi, MA, Rizzardi, MA, Agostinetto, D, Dal Magro, T (2007) Conyza bonariensis biotypes resistant to the glyphosate in Southern Brazil. Planta Daninha. 25:573578
Vidal, RA, Fleck, NC (1997) Análise do risco da ocorrência de biótipos de plantas daninhas resistentes aos herbicidas. Planta Daninha. 15:152161
Weaver, SE (2001) The biology of Canadian weeds; Conyza canadensis . Canadian J Plant Sci. 4:867875
Wright, S (1965) The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution. 19:395399
Wright, S (1978) Evolution and the Genetics of Population, Variability Within and Among Natural Populations. Chicago University of Chicago Press, 590 p
Xiao-ling, S, Jia-jun, W, Hong-jun, Z, Sheng, Q (2011) Occurrence of glyphosate-resistant horseweed (Conyza canadensis) population in China. Agricult Sci China. 10:10491055
Yamauti, MS, Barroso, AAM, Souza, MC, Alves, PLCA (2010) Chemical control of glyphosate-resistant horseweed (Conyza canadensis) and hairy fleabane (Conyza bonariensis) biotypes. Rev Cienc Agron. 41:495500
Yeh, FC, Yang, R, Boyle, T (1999) Popgene version 1.32: Microsoft Windows-based freeware for population genetic analysis. Calgary, AB Centre for International Forestry Research, University of Alberta
Zelaya, I, Owen, MDK, VanGessel, MJ (2004) Inheritance of evolved glyphosate resistance in Conyza canadensis (L.) Cronq. Theor Appl Gen. 110:5870
Zelaya, IA, Owen, MDK, VanGessel, MJ (2007) Transfer of glyphosate resistance: evidence of hybridization in Conyza (Asteraceae). Am J Bot. 94:660673
Zheng, D, Kruger, GR, Singh, S, Davis, VM, Tranel, PJ, Weller, SC, Johnson, WG (2011) Cross-resistance of horseweed (Conyza canadensis) populations with three different ALS mutations. Pest Manag Sci. 67:14861492

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed