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Evaluation of the Interaction between Glyphosate and Glufosinate

  • Rachel K. Bethke (a1), William T. Molin (a2), Christy Sprague (a1) and Donald Penner (a1)


Crops transformed to provide resistance to herbicides with two different mechanisms of action provide new opportunities for control of herbicide-resistant weeds. However, unexpected interactions may develop, especially for herbicides not generally used in tank-mixtures. The objectives of this study were to evaluate weed control and determine herbicide interactions and fluorescence responses with combinations of glyphosate and glufosinate on selected weeds prevalent in Michigan cropping systems. Field studies to determine herbicide interactions resulted in synergism only at 0.84 kg ae ha−1 of glyphosate and 0.47 kg ai ha−1 glufosinate in 2008. Early synergism (7 d after treatment [DAT]) was observed in the field at several combined rates for common lambsquarters and velvetleaf in 2009, and in the greenhouse for giant foxtail. Differences between years were perhaps due to the effect of environmental conditions on herbicide absorption and translocation. Antagonism was observed in the field in 2009 for velvetleaf, common lambsquarters, and giant foxtail especially at 840 g ae ha−1 glyphosate and 118 g ai ha−1 glufosinate, 28 DAT. Antagonism was also observed in the greenhouse for giant foxtail and Canada thistle, 28 DAT. Fluorescence measurements on Canada thistle in the greenhouse showed that glufosinate and glufosinate plus glyphosate acted rapidly to quench electron transport of photosystem II (PS II) system of photosynthesis, and the fluorescence characteristics of the glyphosate and glufosinate combinations were indistinguishable from glufosinate alone.


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Abbaspoor, M. and Streibig, J. C. 2005. Clodinafop changes the chlorophyll fluorescence induction curve. Weed Sci. 53:19.
Appleby, A. P. and Somabhi, M. 1978. Antagonistic effect of atrazine and simazine on glyphosate activity. Weed Sci. 26:135139.
Barbagallo, R. P., Oxborough, K., Pallet, K. E., and Baker, N. R. 2003. Rapid, non-invasive screening for perturbations of metabolism and plant growth using chlorophyll fluorescence imaging. Plant Physiol. 132:485493.
Butler, W. L. 1978. Energy distribution in the photochemical apparatus of photosynthesis. Annu. Rev. Plant Physiol. 29:345378.
Christensen, M. G., Teicher, H. B., and Streibig, J. C. 2003. Linking fluorescence induction curve and biomass in herbicide screening. Pest Manage. Sci. 59:13031310.
Chuah, T. S., Tehs, H. H., Cha, T. S., and Ismail, B. S. 2008. Antagonism of glufosinate ammonium activity caused by glyphosate in the tank mixture used for control of goosegrass (Eleusine indica Gaertn.). Plant Prot. Quart. 23:116119.
Colby, S. R. 1967. Calculating synergistic and antagonistic responses of herbicide combinations. Weeds 15:2022.
Frankart, C., Eullaffroy, P., and Vernet, G. 2003. Comparative effects of four herbicides on non-photochemical fluorescence quenching in Lemna minor . Environ. Exp. Bot. 49:159168.
Hayward, D. M., Colby, S. R., and Parham, M. R. 1988. Enhancement of paraquat activity with photosynthetic inhibitors. Abstr. Weed Sci. Soc. Am. 28:5.
Hugh, B. J. and Reboud, X. 2009. Selecting for weed resistance: herbicide rotation and mixture. Weed Technol. 23:363370.
Hydrick, D. E. and Shaw, D. R. 1994. Effects of tank-mix combinations of non-selective foliar and selective soil-applied herbicides on three weed species. Weed Technol. 8:129133.
Kirkwood, R. C., Hetherington, R., Reynolds, T. L., and Marshall, G. 2000. Absorption, localization, translocation and activity of glyphosate in barnyardgrass (Echinochloa crus-galli (L) Beauv): influence of herbicide and surfactant concentration. Pest Manage. Sci. 56:359367.
Kudsk, P. and Mathiassen, S. K. 2004. Joint action of amino acid biosynthesis-inhibiting herbicides. Weed Res. 44:313322.
Lich, J. M., Renner, K. A., and Penner, D. 1997. Interaction of glyphosate with postemergence soybean (Glycine max) herbicides. Weed Sci. 45:1221.
Olesen, C. F. and Cedergreen, N. 2010. Glyphosate uncouples gas exchange and chlorophyll fluorescence. Pest Manage. Sci. 66:536542.
Percival, M. P. and Baker, N. R. 1991. Herbicides and photosynthesis. Pages 126 in Baker, N. R. and Percival, M. P., eds. Herbicides. London Elsevier.
Ralph, P. J. 2000. Herbicide toxicity of Halophila ovalis assessed by chlorophyll A fluorescence. Aquat. Bot. 66:141152.
Strasser, R. J., Sirivastava, A., and Tsimilli-Michael, M. 2000. The fluorescence transient as a tool to characterize and screen photosynthetic samples. Pages 445483 in Yunus, M., Pathre, U., and Mohanty, P., eds. Probing Photosynthesis: Mechanisms, Regulation and Adaptation. London Taylor & Francis.
Wehtje, G., Altland, J. E., and Gilliam, C. H. 2008. Interaction of glyphosate and diquat in ready-to-use weed control products. Weed Technol. 22:472476.



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