Experiments were conducted in a growth chamber to determine the effects of planting depth on the developmental sequence of velvetleaf seedlings and the effect of cyanazine placement relative to velvetleaf planting depth on cyanazine toxicity. Velvetleaf seedling emergence was delayed when seeds were planted 6 cm deep compared to the 2- and 4-cm planting depths. Lateral roots did not appear until after the cotyledons had emerged and expanded. This was followed by secondary root emergence from the primary root which occurred before adventitious roots appeared from the hypocotyl for the 2- and 4-cm planting depths. The first true leaf did not unfold until after the secondary root system was well developed. The quantity of adventitious roots on the hypocotyl increased with increasing planting depth. Velvetleaf adventitious roots appeared to be involved in cyanazine toxicity when cyanazine was placed above the seed. Increasing planting depth increased the proportion of seedling absorptive tissue above compared to below the seed. This resulted in increased cyanazine exposure for the deeper planted velvetleaf seeds when herbicide was placed above the seed.