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Diverse Mutations in the Acetolactate Synthase Gene Confer Chlorsulfuron Resistance in Kochia (Kochia scoparia) Biotypes

  • Mary J. Guttieri (a1), Charlotte V. Eberlein (a1) and Donald C. Thill (a2)

Abstract

The DNA sequence of an 83-base pair region of the acetolactate synthase (ALS) gene was compared for 10 chlorsulfuron-resistant (R) and three chlorsulfuron-susceptible (S) kochia biotypes. Point mutation in the codon for the proline residue at position 173 in Domain A of the ALS protein was observed in seven of 10 R biotypes. Among these seven R biotypes, mutation to threonine, serine, arginine, leucine, glutamine, and alanine was identified. The mechanism of resistance was determined for the R biotypes that did not have mutation in Domain A; all were resistant due to modified ALS, which indicated that at least one non-Domain A mutation site for resistance exists in kochia. Sequence results indicate that multiple mutations for resistance have occurred, and that geographically widespread ALS-inhibitor resistance in kochia is not the result of a single resistance allele.

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References

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Keywords

Diverse Mutations in the Acetolactate Synthase Gene Confer Chlorsulfuron Resistance in Kochia (Kochia scoparia) Biotypes

  • Mary J. Guttieri (a1), Charlotte V. Eberlein (a1) and Donald C. Thill (a2)

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