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Determination of ACCase Sensitivity and Gene Expression in Quizalofop–Ethyl-Resistant and -Susceptible Barnyardgrass (Echinochloa crus-galli) Biotypes

Published online by Cambridge University Press:  20 January 2017

Zhibo Huan ,
Zhi Xu ,
Daizhu Lv  and
Jinxin Wang
Zhibo Huan
Affiliation:
Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, P. R. China Plant Protection College, Shandong Agricultural University, Tai'an 271018, P. R. China
Zhi Xu
Affiliation:
Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, P. R. China
Daizhu Lv
Affiliation:
Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, P. R. China
Jinxin Wang*
Affiliation:
Plant Protection College, Shandong Agricultural University, Tai'an 271018, P. R. China
*
Corresponding author's E-mail: wangjx@sdau.edu.cn
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Abstract

Mechanisms of herbicide resistance were studied in a quizalofop–ethyl-resistant barnyardgrass biotype. Acetyl-coenzyme A carboxylase (ACCase) sensitivity to quizalofop-p-ethyl was measured by high-performance liquid chromatography and the trend in ACCase gene expression over time was determined using real-time polymerase chain reaction. The results showed that an insensitive ACCase was present in Geqiushan resistant plants (R), with a resistance index of 106. The basal ACCase activities in Geqiushan R and Geqiushan susceptible plants (S) were similar, at 1.20 and 1.17 ng malonyl-CoA min−1 µg−1 extract protein, respectively. Basal ACCase gene expression in Geqiushan R was similar to that in Geqiushan S. The relative expression of ACCase gene decreased after spraying quizalofop–ethyl at 60 g ai ha−1 in Geqiushan S, whereas it was almost not changed in Geqiushan R. From these results we concluded that plastid ACCase sensitivity change might be responsible for the resistance and gene overexpression does not play a role in this resistance.

Keywords

Quizalofop-P-ethylbarnyardgrass, Echinochloa crus-galli (L). Beauv. ECHCGHPLCreal-time PCR
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 61 , Issue 4 , December 2013 , pp. 537 - 542
Copyright
Copyright © Weed Science Society of America 

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