Skip to main content Accessibility help
×
Home

Cross- and multiple resistance of diclofop-resistant Lolium spp.

  • Yong-In Kuk (a1), Nilda R. Burgos and Ronald E. Talbert (a1)

Abstract

Eighteen Lolium spp. (ryegrass) accessions collected in 1998 from several locations in Arkansas were tested for resistance (R) to diclofop in both seed and whole-plant response bioassays. Eleven accessions were L. temulentum and eight were L. perenne. Fourteen of eighteen accessions were confirmed resistant to diclofop in whole-plant assay. Three of the susceptible (S) accessions were L. temulentum. The GR50 (diclofop concentration that reduced shoot or root length by 50%) R/S ratios based on whole-plant response were greater than those of the seed bioassay in all test populations, indicating that the whole-plant bioassay was more sensitive than the seed bioassay for determining diclofop resistance in Lolium spp. The most resistant (#18) and most susceptible (#3) accessions of L. temulentum were used for multiple resistance and enzyme assay experiments. Based on whole-plant bioassay, accession #18 was 411 times more resistant to diclofop than the susceptible accession #3. Accession #18 exhibited cross-resistance to fenoxaprop and multiple resistance to chlorsulfuron applied preemergence or postemergence. Resistance to other herbicide families was not observed. Resistance to chlorsulfuron was not detected in the seed bioassay. Acetyl-CoA carboxylase (ACCase) from accession #18 was 833 times more resistant to diclofop and 10 times more resistant to sethoxydim than ACCase from accession #3. Cross-resistance to sethoxydim was not observed at the whole-plant level. Resistance to diclofop among Lolium spp. from Arkansas may be due to an alteration in the target enzyme, ACCase.

Copyright

Corresponding author

Corresponding author. Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 276 Altheimer Drive, Fayetteville, AR 72704; nburgos@comp.uark.edu

References

Hide All
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal. Biochem. 72:248254.
Bradley, K. W., Wu, J., Hagood, E. S., and Hatzios, K. K. 1999. Investigations on the mechanism of resistance to sethoxydim and quizalofop in a biotype of johnsongrass (Sorghum halepense) from Virginia. Weed Sci. Soc. Am. Abstr. 39:79.
Brewster, B. D., Appleby, A. P., and Spinner, R. L. 1977. Control of Italian ryegrass and wild oats in winter wheat with HOE 23408. Agron. J. 69:911913.
Burnet, M.W.M., Barr, A. R., and Powles, S. B. 1994a. Chloroacetamide resistance in rigid ryegrass (Lolium rigidum). Weed Sci. 42:153157.
Burnet, M.W.M., Hart, Q., Holtum, J.A.M., and Powles, S. B. 1994b. Resistance to nine herbicide classes in a population of rigid ryegrass (Lolium rigidum). Weed Sci. 42:369377.
Burnet, M.W.M., Hildebrand, O. B., Holtum, J.A.M., and Powles, S. B. 1991. Amitrole, triazine, substituted urea, and metribuzin resistance in a biotype of rigid ryegrass (Lolium rigidum). Weed Sci. 39:317323.
Burnet, M.W.M., Loveys, B. R., Holtum, J.A.M., and Powles, S. B. 1993. A mechanism of chlorotoluron resistance in Lolium rigidum . Planta 190:182189.
Christopher, J. T., Powles, S. B., Holtum, J.A.M., and Liljegren, D. R. 1991. Cross resistance to herbicides in annual ryegrass (Lolium rigidum) II. Chlorsulfuron resistance involves a wheat-like detoxification system. Plant Physiol. 95:10361043.
Christopher, J. T., Powles, S. B., Liljegren, D. R., and Holtum, J.A.M. 1992. Resistance to acetolactate synthase inhibitors in annual ryegrass (Lolium rigidum) involves at least two mechanisms. Plant Physiol. 100:19091913.
Cotterman, J. C. and Saari, L. L. 1992. Rapid metabolic inactivation is the basis for cross-resistance to chlorsulfuron in diclofop-methyl-resistant rigid ryegrass (Lolium rigidum) biotype SR4/84. Pestic. Biochem. Physiol. 43:182192.
De Prado, R. D., De Prado, J. L., and Menendez, J. 1997. Resistance to substituted urea herbicides in Lolium rigidum biotypes. Pestic. Biochem. Physiol. 57:126136.
Frie, J. B., Zollinger, R. K., Manthey, F. A., and Durgan, B. R. 1993. Wild oat (Avena fatua L.) resistance to diclofop in Red River Valley. Proc. North Cent. Weed Sci. Soc. 48:16.
Gronwald, J. W., Eberlein, C. V., Betts, K. J., Baerg, R. J., Ehlke, N. J., and Wyse, D. L. 1992. Mechanism of diclofop resistance in an Italian ryegrass (Lolium multiflorum Lam.) biotype. Pestic. Biochem. Physiol. 44:126139.
Heap, I. M. and Knight, R. 1982. A population of ryegrass tolerant to the herbicide diclofop-methyl. J. Aust. Inst. Agric. Sci. 48:156157.
Heap, I. M. and Knight, R. 1986. The occurrence of herbicide cross resistance in a population of annual ryegrass, Lolium rigidum, resistant to diclofop-methyl. Aust. J. Agric. Res. 37:149156.
Heap, I. M. and Knight, R. 1990. Variation in herbicide cross resistance among populations of annual ryegrass (Lolium rigidum) resistant to diclofop-methyl. Aust. J. Agric. Res. 41:121128.
Heap, I. M. and Morrison, I. N. 1996. Resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in green foxtail (Setaria viridis). Weed Sci. 44:2530.
Heap, I. M., Murray, B. G., Loeppky, H. A., and Morrison, I. N. 1993. Resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in wild oat (Avena fatua). Weed. Sci. 41:232238.
Holtum, J.A.M., Matthews, J. M., Häusler, R. E., Liljegreen, D. R., and Powles, S. B. 1991. Cross resistance to herbicides in annual ryegrass (Lolium rigidum). III. On the mechanism of resistance to diclofop-methyl. Plant Physiol. 97:10261034.
Kobek, K., Focke, M., and Lichtenthaler, H. K. 1988. Fatty-acid biosynthesis and acetyl-CoA carboxylase as a target of diclofop, fenoxaprop and other aryloxyphenoxypropionic acid herbicides. Z. Naturforsch. 43c:4754.
Kotoula-Syka. 1999. Annual ryegrass (Lolium rigidum) resistant to ACCase inhibitors from Northern Greece. Weed Sci. Soc. Am. Abstr. 39:78.
Kuk, Y. I., Wu, J., Derr, J. F., and Hatzios, K. K. 1999. Mechanism of resistance in a biotype of smooth crabgrass (Digitaria ischaemum), which developed resistance to fenoxaprop. Pestic. Biochem. Physiol. 64:112123.
Leach, G. E., Devine, M. D., Kirkwood, R. C., and Marshall, G. 1995. Target enzyme-based resistance to acetyl-coenzyme A carboxylase inhibitors in Eleusine indica . Pestic. Biochem. Physiol. 51:129136.
Letouzè, A. and Gasquez, J. 1999. A rapid reliable test for screening aryloxyphenoxypropionic acid resistance within Alopecurus myosuroides and Lolium spp. populations. Weed Res. 39:3748.
Ma, G., Burton, J. D., and Coble, H. D. 1998. Herbicide inhibition and characterization of acetyl-coenzyme A carboxylase from diclofop-susceptible and -resistant Italian ryegrass (Lolium multiflorum Lam.) of NC biotype. Weed Sci. Soc. Am. Abstr. 38:51.
Maneechote, C., Holtum, J.A.M., Preston, C., and Powles, S. B. 1994. Resistant acetyl-CoA carboxylase is a mechanism of herbicide resistance in a biotype of Avena sterilis ssp. ludoviciana . Plant Cell Physiol. 35:627635.
Marles, M.A.S., Devine, M. D., and Hall, J. C. 1993. Herbicide resistance in Setaria viridis conferred by a less sensitive form of acetyl coenzyme A carboxylase. Pest. Biochem. Physiol. 46:714.
Matthews, J. M., Holtum, J.A.M., Liljegren, D. R., Furness, B., and Powles, S. B. 1990. Cross resistance to herbicides in annual ryegrass (Lolium rigidum) I. Properties of the herbicide targets acetyl-coenzyme A carboxylase and acetolactate synthase. Plant Physiol. 94:11801186.
Mcalister, F. M., Holtum, J.A.M., and Powles, S. B. 1995. Dinitroaniline herbicide resistance in rigid ryegrass (Lolium rigidum). Weed Sci. 43:5562.
Michael, W.M.B., Loveys, B. R., Holtum, J.A.M., and Powles, S. B. 1993. Increased detoxification is a mechanism of simazine resistance in Lolium rigidum . Pestic. Biochem. Physiol. 46:207218.
Powles, S. B., Lorraine-Colwill, D. F., Dellow, J. J., and Preston, C. 1998. Evolved resistance to glyphosate in rigid ryegrass (Lolium rigidum) in Australia. Weed Sci. 46:604607.
Preston, C., Tardif, F. J., Christopher, J. T., and Powles, S. B. 1996. Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pestic. Biochem. Physiol. 54:123134.
[SAS] Statistical Analysis System. 1998. SAS/STAT User's Guide. Version 7. Cary, NC: Statistical Analysis Systems Institute. Electronic version.
Seefeldt, S. S., Fuerst, E. P., Gealy, D. R., Shukla, A., Irzyk, G. P., and Devine, M. D. 1996. Mechanisms of resistance to diclofop of two wild oat (Avena fatua) biotypes from the Willamette Valley of Oregon. Weed Sci. 44:776781.
Seefeldt, S. S., Gealy, D. R., Brewster, B. D., and Fuerst, E. P. 1994. Cross resistance of several diclofop-resistant wild oat (Avena fatua) biotypes from the Willamette Valley of Oregon. Weed Sci. 42:430437.
Shimabukuro, R. H. and Hoffer, B. L. 1991. Metabolism of diclofop-methyl in susceptible and resistant biotypes of Lolium rigidum . Pestic. Biochem. Physiol. 39:251260.
Shukla, A., Dupont, S., and Devine, M. D. 1997a. Resistance to ACCase-inhibitor herbicides in wild oats: evidence for target site-based resistance in two biotypes from Canada. Pestic. Biochem. Physiol. 57:147155.
Shukla, A., Leach, G. E., and Devine, M. D. 1997b. High-level resistance to sethoxydim conferred by an alteration in the target enzyme, acetyl-CoA carboxylase, in Setaria faberi and Setaria viridis . Plant Physiol. Biochem. 35:803807.
Smeda, R. J., Barrentine, W. L., and Snipes, C. E. 1993. Johnsongrass (Sorghum halepense (L.) Pers) resistance to postemergence grass herbicides. Weed Sci. Soc. Am. Abstr. 33:18
Smith, E. B. 1994. Keys to the Flora of Arkansas. Fayetville, AR: The University of Arkansas Press. pp. 287, 304.
Stanger, C. E. and Appleby, A. P. 1989. Italian ryegrass (Lolium multiflorum) accessions tolerant to diclofop. Weed Sci. 37:350352.
Stoltenberg, D. E. and Wiederholt, R. J. 1995. Giant foxtail (Setaria faberi) resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides. Weed Sci. 43:527535.
Tal, A., Zarka, S., and Rubin, B. 1996. Fenoxaprop-P resistance in Phalaris minor conferred by an insensitive acetyl-coenzyme A carboxylase. Pestic. Biochem. Physiol. 56:134140.
Tardif, F. J., Holtum, J.A.M., and Powles, S. B. 1993. Occurrence of a herbicide-resistant acetyl-coenzyme A carboxylase mutant in annual ryegrass (Lolium rigidum) selected by sethoxydim. Planta 190:176181.
Terrell, E. E. 1966. Taxonomic implications of genetics in ryegrass (Lolium). Bot. Rev. 32:138164.
Wiederholt, R. J. and Stoltenberg, D. E. 1995. Cross resistance of a large crabgrass (Digitaria sanguinalis) accession to aryloxyphenoxypropionate and cyclohexanedione herbicides. Weed Technol. 9:518524.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed