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Confirmation of Flixweed (Descurainia sophia) Resistance to Tribenuron in China

  • Hai Lan Cui (a1), Chao Xian Zhang (a1), Hong Jun Zhang (a2), Xue Liu (a2), Yan Liu (a1), Gui Qi Wang (a3), Hong Juan Huang (a1) and Shou Hui Wei (a1)...

Abstract

Reports arose from major Chinese wheat production regions that flixweed was not controlled by tribenuron after the herbicide was continuously used for several years. Flixweed seeds were collected from wheat fields that had been treated with tribenuron repeatedly over 3 to 15 yr or from road sides and remote hills that had never received tribenuron in Jiangsu, Hebei, Shanxi, Sichuan, Shandong, Shaanxi, and Henan provinces, and Tianjin and Beijing metropolises in China. The response of various biotypes to tribenuron was determined by whole plant experiments in the greenhouse. The experiments demonstrated that 11 of 32 flixweed biotypes were susceptible to tribenuron. The remaining 21 biotypes expressed moderate to high levels of tribenuron resistance with resistance indices ranging from 4 to > 1,500. DNA sequence analysis of acetolactate synthase (ALS) genes of selected biotypes 6, 7, 27, and 29 revealed a point mutation at position 197 of the ALS gene, as numbered relative to the protein sequence of Arabidopsis, where proline was substituted by leucine in biotype 7 and by threonine in biotype 29. These mutations are known to confer resistance to ALS-inhibiting herbicides and are responsible for the high resistance of these biotypes to tribenuron. The results also indicate that tribenuron resistance in flixweed is widespread in China and management programs to control these resistant populations are warranted.

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Corresponding author

Corresponding author's E-mail: cxzhang@wssc.org.cn

References

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Annual Book of Agriculture in China 2006. Beijing, China China Agricultural Press (in Chinese). 508 p.
Beckie, H. J., Hall, L. M., Tardif, F. J., and Seguin-Swartz, G. 2007. Acetolactate synthase inhibitor-resistant stinkweed (Thlaspi arvense L.) in Alberta. Can. J. Plant Sci. 87:965972.
Bernasconi, P., Woodworth, A. R., Rosen, B. A., Subramanian, M. V., and Siehl, D. L. 1995. A naturally occurring point mutation confers broad range tolerance to herbicides that target acetolactate synthase. J. Biol. Chem. 270:17,38117,385.
Chaleff, R. S. and Mauvais, C. J. 1984. Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science. 224:14431445.
Christopher, J. T., Powles, S. B., and Holtum, J. A. M. 1992. Resistance to acetolactate synthase-inhibiting herbicides in annual ryegrass (Lolium rigidum) involves at least two mechanisms. Plant Physiol. 100:19091913.
Corbett, C-A. L. and Tardif, F. J. 2006. Detection of resistance to acetolactate synthase inhibitors in weeds with emphasis on DNA-based techniques: a review. Pest Manag. Sci. 62:584597.
Doyle, J. J. and Doyle, J. L. 1990. Isolation of plant DNA from fresh tissue. Focus. 12:1315.
Durner, J., Gailus, V., and Boger, P. 1990. New aspects on inhibition of plant acetolactate synthase by chlorsulfuron and imazaquin. Plant Physiol. 95:11441149.
Gerwick, B. C., Subramanian, M. V., and Loney-Gallant, V. I. 1990. Mechanism of action of the 1,2,4-triazolo[1,5-a] pyrimidines. Pestic. Sci. 29:357364.
Heap, I. 2008. The International Survey of Herbicide Resistant Weeds. http://www.weedscience.com. Accessed: February 26, 2008.
[ICAMA] Institute for Control of Agrichemicals, Ministry of Agriculture 1988. The Bulletins of the Pesticide Registration in China. Beijing, China China Agricultural Press (in Chinese). 166 p.
Kudsk, P., Mathiassen, S. K., and Cotterman, J. C. 1995. Sulfonylurea resistance in Stellaria media (L.) Vill. Weed Res. 35:1924.
McNaughton, K. E., Letarte, J., Lee, E. A., and Tardif, F. J. 2005. Mutations in ALS confer herbicide resistance in redroot pigweed (Amaranthus retroflexus) and Powell amaranth (Amaranthus powellii). Weed Sci. 53:1722.
Moodie, M., Finch, R. P., and Marshall, G. 1997. Analysis of genetic variation in wild mustard (Sinapis arvensis) using molecular markers. Weed Sci. 45:102107.
Patzoldt, W. L. and Tranel, P. J. 2002. Molecular analysis of cloransulam resistance in a population of giant ragweed. Weed Sci. 50:299305.
Preston, C. and Mallory-Smith, C. A. 2001. Biochemical mechanisms, inheritance, and molecular genetics of herbicide resistance in weeds. Pages 2360. in. S. B. Powles and D. L. Shaner, eds. Herbicide Resistance and World Grains. Boca Raton, FL CRC.
Preston, C., Stone, L. M., Rieger, M. A., and Baker, J. 2006. Multiple effects of a naturally occurring proline to threonine substitution within acetolactate synthase in two herbicide-resistant populations of Lactuca serriola. Pestic. Biochem. Physiol. 84:227235.
Rajguru, S. N., Burgos, N. R., Shivrain, V. K., and Stewart, J. McD. 2005. Mutations in the red rice ALS gene associated with resistance to imazethapyr. Weed Sci. 53:567577.
Saari, L. L., Cotterman, J. C., Smith, W. F., and Primiani, M. M. 1992. Sulfonylurea herbicide resistance in common chickweed, perennial ryegrass, and Russian thistle. Pestic. Biochem. Physiol. 42:110118.
Saari, L. L., Cotterman, J. C., and Thill, D. C. 1994. Resistance to acetolactate synthase inhibiting herbicide. Pages 83139. in. S. B. Powles and J.A.M. Holtum, eds. Herbicide Resistance in Plants, Biology and Biochemistry. Boca Raton, FL Lewis.
Santel, H. J., Bowden, B. A., Sorensen, V. M., Mueller, K. H., and Reynolds, J. 1999. Flucarbazone-sodium: a new herbicide for grass control in wheat. Weed Sci. Soc. Am. Abstr. 39:7.
Shaner, D. L. 1999. Resistance to acetolactate synthase (ALS) inhibitors in the United States: history, occurrence, detection, and management. Weed Sci. 44:405411.
Shaner, D. L., Anderson, P. C., and Stidham, M. A. 1984. Imidazolinones: potential inhibitors of acetohydroxyacid synthase. Plant Physiol. 76:545546.
Shi, C. X. and Che, J. Y. 1993. Studies on wheat yield loss caused by flixweed (Descurainia sophia). Shaanxi J. Agric. Sci. 3:2123. [in Chinese].
Smit, J. J. and Cairns, A. L. P. 2001. Resistance of Raphanus raphanistrum to chlorsulfuron in the Republic of South Africa. Weed Res. 41:4147.
Stidham, M. A. 1991. Herbicides that inhibit acetohydroxyacid synthase. Weed Sci. 39:428434.
Stromme, K. M., Horsman, G. P., and Devine, M. D. 1998. Resistance to acetolactate synthase inhibitors and quinclorac in a biotype of false cleavers (Galium spurium). Weed Sci. 46:390396.
Tranel, P. J. and Wright, T. R. 2002. Resistance of weeds to ALS-inhibiting herbicides: what have we learned. Weed Sci. 50:700712.
Tranel, P. J., Wright, T. R., and Heap, I. M. 2008. ALS mutations from herbicide-resistant weeds. http://www.weedscience.com. Accessed March 02, 2008.
Umbarger, H. E. 1978. Amino acid biosynthesis and its regulation. Ann. Rev. Biochem. 47:533606.
Walsh, M. J., Owen, M. J., and Powles, S. B. 2007. Frequency and distribution of herbicide resistance in Raphanus raphanistrum populations randomly collected across the Western Australian wheatbelt. Weed Res. 47:542550.
Wang, G. X., Tan, M. K., Rakshit, S., Saitoh, H., Terauchi, R., Imaizumi, T., Ohsako, T., and Tominaga, T. 2007. Discovery of single-nucleotide mutations in acetolactate synthase genes by Ecotilling. Pestic. Biochem. Physiol. 88:143148.
Whaley, C. M., Wilson, H. P., and Westwood, J. H. 2007. A new mutation in plant ALS confers resistance to five classes of ALS-inhibiting herbicides. Weed Sci. 55:8390.
Woodworth, A., Bernasconi, P., Subramanian, M., and Rosen, B. 1996. A second naturally occurring point mutation confers broad-based tolerance to acetolactate synthase inhibitors. Plant Physiol. 111 S105.
Yu, Q., Zhang, X. Q., Hashem, A., Walsh, M. J., and Powles, S. B. 2003. ALS gene proline (197) mutations confer ALS herbicide resistance in eight separated wild radish (Raphanus raphanistrum) populations. Weed Sci. 51:831838.

Keywords

Confirmation of Flixweed (Descurainia sophia) Resistance to Tribenuron in China

  • Hai Lan Cui (a1), Chao Xian Zhang (a1), Hong Jun Zhang (a2), Xue Liu (a2), Yan Liu (a1), Gui Qi Wang (a3), Hong Juan Huang (a1) and Shou Hui Wei (a1)...

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