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Comparative Detoxification of Chlorsulfuron in Leaf Disks and Cell Cultures of Two Perennial Weeds

Published online by Cambridge University Press:  12 June 2017

Beth A. Swisher  and
Monte R. Weimer
Beth A. Swisher
Affiliation:
Dep. Agron., Univ. Nebraska, Lincoln, NE 68583
Monte R. Weimer
Affiliation:
Dep. Agron., Univ. Nebraska, Lincoln, NE 68583
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Abstract

Phytotoxicity of chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino] carbonyl] benzenesulfonamide} in cell cultures of Canada thistle [Cirsium arvense (L.) Scop. # CIRAR] was approximately 30-fold greater than in leafy spurge (Euphorbia esula L. # EPHES) cultures. Differences in chlorsulfuron phytotoxicity in these two species were attributed to large differences in cellular metabolism of the herbicide. Leafy spurge cells metabolized all of the applied 14C-chlorsulfuron within 72 h of treatment, while Canada thistle metabolized less than 2%. Acid hydrolysis of the metabolic products isolated from leaf disks and cell cultures of leafy spurge yielded a radioactive product that cochromatographed with a 2-chlorobenzenesulfonamide standard. Therefore, metabolic transformation occurred on the heterocyclic portion of the molecule. A major metabolite was further characterized as 2-chloro-N-[[4-(hydroxymethyl)-6-methoxy-1,3,5-triazin-2-yl]aminocarbonyl]-benzenesulfonamide by cochromatography with the authentic standard.

Keywords

Herbicide detoxificationherbicide metabolismpesticide metabolismCirsium arvenseEuphorbia esulaCIRAREPHES
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 34 , Issue 4 , July 1986 , pp. 507 - 512
Copyright
Copyright © 1986 by the Weed Science Society of America 

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References

Literature Cited

1. Donald, W. W. 1984. Chlorsulfuron effects on shoot growth and root buds of Canada thistle. Weed Sci. 32:42–50.Google Scholar
2. Dusky, J. A., Davis, D. G., and Shimabukuro, R. H. 1980. Metabolism of diclofop-methyl in cell suspensions of diploid wheat. Physiol. Plant. 49:151–156.Google Scholar
3. Gamborg, O. L., Miller, R. A., and Ojima, K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158.Google Scholar
4. Hageman, L. H. and Behrens, R. 1984. Basis for response differences of two broadleaf weeds to chlorsulfuron. Weed Sci. 32:162–167.CrossRefGoogle Scholar
5. Hutchison, J. M., Shapiro, R., and Sweetser, P. B. 1984. Metabolism of chlorsulfuron by tolerant broadleaves. Pestic. Biochem. Physiol. 22:243–247.Google Scholar
6. Lamoureux, G. L., Gouot, J. M., Davis, D. G., and Rusness, D. G. 1981. Pentachloronitrobenzene metabolism in plants. 3. Metabolism in peanut cell suspension cultures. J. Agric. Food Chem. 29:996–1002.CrossRefGoogle Scholar
7. Levitt, G. 1983. Sulfonylureas: new high potency herbicides. Pages 243–250 in Miyamota, J. et al., eds. ICPAC Pesticide Chemistry: Human Welfare and the Environment. Pergamon Press, Oxford, England.CrossRefGoogle Scholar
8. Messersmith, C. G. and Lym, R. G. 1980. Herbicide and plant growth regulator screening trial on leafy spurge. Res. Rep. North Cent. Weed Control Conf. 60–61.Google Scholar
9. Mumma, R. O. and Hamilton, R. H. 1979. Xenobiotic metabolism in higher plants: In vitro tissue and cell culture techniques. ACS Symp. Ser. 97:35–77.CrossRefGoogle Scholar
10. Oswald, T. H., Smith, A. E., and Phillips, D. V. 1978. Phytotoxicity and detoxification of metribuzin in dark-grown suspension cultures of soybean. Pestic. Biochem. Physiol. 8:73–83.CrossRefGoogle Scholar
11. Ray, T. B. 1984. Site of action of chlorsulfuron. Plant Physiol. 75:827–831.Google Scholar
12. Ray, T. B. and Still, C. C. 1975. Propanil metabolism in rice: a comparison of propanil amidase activities in rice plants and callus cultures. Pestic. Biochem. Physiol. 5:171–177.CrossRefGoogle Scholar
13. Scheel, D. and Sanderman, H. Jr. 1977. Metabolism of DDT and kelthane in cell suspension cultures of parsley and soybean. Planta 133:315–320.Google Scholar
14. Peterson, P. J. and Swisher, B. A. 1985. Absorption, translocation, and metabolism of 14C-chlorsulfuron in Canada thistle. Weed Sci. 33:7–11.CrossRefGoogle Scholar
15. Sweetser, P. B., Schow, G. S., and Hutchison, J. M. 1982. Metabolism of chlorsulfuron by plants: biological basis for selectivity of a new herbicide for cereals. Pestic. Biochem. Physiol. 17:18–23.Google Scholar