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Chlorsulfuron Tolerance and Acetolactate Synthase Activity in Corn (Zea mays L.) Inbred Lines

  • Giuseppe Forlani (a1), Erik Nielsen (a1), Pierangelo Landi (a2) and Roberto Tuberosa (a2)

Abstract

Seven corn inbred lines previously shown to differ in response to soil residues of chlorsulfuron were characterized as to the target-enzyme acetolactate synthase (ALS) specific activity and to its susceptibility to the herbicide. ALS from plantlets at the five-leaf stage of growth was similarly susceptible to chlorsulfuron in all lines and its specific activity in the shoots was not significantly correlated with in vivo tolerance to the herbicide. By contrast, differences in ALS specific activity in roots of plants both at the five- and three-leaf stages of growth were significantly correlated (r = 0.96∗∗ and r = 0.93∗∗, respectively) with in vivo tolerance. Correlation was also noted in extracts from cultured excised root tips (r = 0.94∗∗). Callus tissue of a chlorsulfuron-tolerant line was less affected by the herbicide and had a significantly higher ALS specific activity than callus from a chlorsulfuron susceptible line, whereas inhibition of ALS by the herbicide was similar in both lines. These results indicate that the naturally occurring differences in ALS levels in the roots of the investigated inbred lines contribute largely to the differential in vivo response observed to chlorsulfuron.

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1. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248254.
2. Chaleff, R. S. and Ray, T. B. 1984. Herbicide-resistant mutants from tobacco cell cultures. Science 223:11481151.
3. Chu, C. C., Wang, C. C., Sun, C. S., Hsu, C., Yin, K. C., Chu, C. Y., and Bi, F. Y. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on nitrogen sources. Sci. Sin. 18:659668.
4. Donn, G., Tischer, E., Smith, J. A., and Goodman, H. M. 1984. Herbicide-resistant alfalfa cells: an example of gene amplification in plants. J. Mol. Appl. Genet. 2:621635.
5. Eberlein, C. V., Rosow, K. M., Geadelmann, J. L., and Openshaw, S. J. 1989. Differential tolerance of corn genotypes to DPX-M6316. Weed Sci. 37:651657.
6. Landi, P., Vicari, A., and Catizone, P. 1989. Response of maize (Zea mays L.) inbred lines and hybrids to chlorsulfuron. Weed Res. 29:265271.
7. Nafziger, E. D., Widholm, J. M., Steinrücken, H. C., and Killmer, J. L. 1984. Isolation and characterization of a carrot cell line tolerant to glyphosate. Plant Physiol. 76:571574.
8. Peterson, M. A. and Arnold, W. E. 1985. Response of rotational crops to soil residue of chlorsulfuron. Weed Sci. 34:131136.
9. Ray, T. B. 1986. Sulfonylurea herbicides as inhibitors of amino acid biosynthesis in plants. TIBS 11:180183.
10. Reinert, J. and Yeoman, M. M. 1982. Page 78 in Plant Cell and Tissue Culture; a Laboratory Manual. Springer-Verlag, Berlin.
11. Sander, K. W. and Barrett, M. 1989. Differential imazaquin tolerance and behavior in selected corn (Zea mays) hybrids. Weed Sci. 37:290295.
12. Sari Gorla, M., Ottaviano, E., Frascaroli, E., and Landi, P. 1989. Herbicide-tolerant com by pollen selection. Sex. Plant Reprod. 2:6569.
13. Sebastian, S. A. and Chaleff, R. S. 1987. Soybean mutants with increased tolerance for sulfonylurea herbicides. Crop Sci. 27:948952.
14. Smart, C. C., Johanning, D., Muller, G., and Amrhein, N. 1985. Selective overproduction of 5-enol-pyravylshikimic acid 3-phosphate synthase in a plant cell culture which tolerates high doses of the herbicide glyphosate. J. Biol. Chem. 260:1633816346.
15. Snedecor, G. W. and Cochran, W. G. 1967. Pages 159160 in Statistical Methods. 6th ed. Iowa State Univ. Press, Ames, LA.
16. Sweetser, P. B. 1985. Safening of sulfonylurea herbicides to cereal crops: mode of herbicide antidote action. Proc. Br. Crop Prot. Conf.–Weeds 11471154.
17. Westerfeld, W. W. 1945. A colorimetric determination of blood acetoin. J. Biol. Chem. 161:495502.

Keywords

Chlorsulfuron Tolerance and Acetolactate Synthase Activity in Corn (Zea mays L.) Inbred Lines

  • Giuseppe Forlani (a1), Erik Nielsen (a1), Pierangelo Landi (a2) and Roberto Tuberosa (a2)

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