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Characterization of target-site resistance to ALS-inhibiting herbicides in Ammannia multiflora populations

Published online by Cambridge University Press:  05 May 2022

Wei Deng Open the ORCID record for Wei Deng [Opens in a new window] ,
Zhiwen Duan Open the ORCID record for Zhiwen Duan [Opens in a new window] ,
Yang Li Open the ORCID record for Yang Li [Opens in a new window] ,
Hanwen Cui Open the ORCID record for Hanwen Cui [Opens in a new window] ,
Cheng Peng Open the ORCID record for Cheng Peng [Opens in a new window]  and
Shuzhong Yuan Open the ORCID record for Shuzhong Yuan [Opens in a new window]
Wei Deng
Affiliation:
Leuturer, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China
Zhiwen Duan
Affiliation:
Graduate Student, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China
Yang Li
Affiliation:
Graduate Student, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China
Hanwen Cui
Affiliation:
Graduate Student, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China
Cheng Peng
Affiliation:
Graduate Student, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China
Shuzhong Yuan*
Affiliation:
Associate Professor, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China
*
Author for correspondence: Shuzhong Yuan, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China. (Email: yuansz10201@163.com)
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Abstract

Ammannia multiflora Roxb. is a dominant broadleaf weed that is a serious problem in southern China rice fields, and acetolactate synthase (ALS)-inhibiting herbicides have been used for its control for more than 20 years. Excessive reliance on ALS-inhibiting herbicides has led to herbicide resistance in A. multiflora. In this study, 10 A. multiflora populations from the Jiangsu Province of China were collected, and the resistance levels and target site–resistance mechanisms to ALS-inhibiting herbicides bensulfuron-methyl and penoxsulam were investigated. The dose–response assays showed that eight populations evolved resistance to bensulfuron-methyl (9.1- to 90.9-fold) and penoxsulam (5.0- to 103.1-fold). Amplification of ALS genes indicated that there were three ALS genes (AmALS1, AmALS2, and AmALS3) in A. multiflora. Sequence analysis revealed amino acid mutations at Pro-197 in either AmALS1 (Pro-197-Ala, Pro-197-Ser, and Pro-197-His) or AmALS2 (Pro-197-Ser and Pro-197-Arg) in resistant populations, and no mutations were found in AmALS3. Moreover, two independent mutations (Pro-197-Ala in AmALS1 and Pro-197-Ser in AmALS2 or Pro-197-Ala in AmALS1 and Pro-197-Arg in AmALS2) coexisted in two resistant populations, respectively. In addition, the auxin mimic herbicides MCPA and florpyrauxifen-benzyl, the photosystem II inhibitor bentazon, and the protoporphyrinogen oxidase inhibitor carfentrazone-ethyl can effectively control the resistant A. multiflora populations. Our study demonstrates the wide prevalence of ALS inhibitor–resistant A. multiflora populations in Jiangsu Province and the diversity of Pro-197 mutations in ALS genes and provides alternative herbicide options for controlling resistant A. multiflora populations.

Keywords

Bensulfuron-methylpenoxsulam
Type
Research Article
Information
Weed Science , Volume 70 , Issue 3 , May 2022 , pp. 292 - 297
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Copyright
© The Author(s), 2022. Published by Cambridge University Press on behalf of the Weed Science Society of America

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Footnotes

Associate Editor: Vipan Kumar, Kansas State University

References

Bagavathiannan, MV, Norsworthy, JK, Smith, KL, Neve, P (2014) Modeling the simultaneous evolution of resistance to ALS- and ACCase-inhibiting herbicides in barnyardgrass (Echinochloa crus-galli) in Clearfield® rice. Weed Technol 28:89–103 CrossRefGoogle Scholar
Bai, S, Liu, W, Wang, H, Zhao, N, Jia, S, Zou, N, Guo, W, Wang, J (2018) Enhanced herbicide metabolism and metabolic resistance genes identified in tribenuron-methyl resistant Myosoton aquaticum L. J Agric Food Chem 66:9850–9857 CrossRefGoogle ScholarPubMed
Cao, Y, Wei, S, Huang, H, Li, W, Zhang, C, Huang, Z (2021) Target-site mutation and enhanced metabolism confer resistance to thifensulfuron-methyl in a multiple-resistant redroot pigweed (Amaranthus retroflexus) population. Weed Sci 69:161–166 CrossRefGoogle Scholar
Dass, A, Shekhawat, K, Choudhary, AK, Sepat, S, Rathore, SS, Mahajan, G, Chauhan, BS (2017) Weed management in rice using crop competition—a review. Crop Prot 95:45–52 CrossRefGoogle Scholar
Deng, W, Yang, M, Duan, Z, Peng, C, Xia, Z, Yuan, S (2021) Molecular basis of resistance to bensulfuron-methyl and cross-resistance patterns to ALS-inhibiting herbicides in Ludwigia prostrata . Weed Technol 35:656–661 CrossRefGoogle Scholar
Deng, W, Yang, Q, Zhang, YZ, Jiao, HT, Mei, Y, Li, XF, Zheng, MQ (2017) Cross resistance patterns to acetolactate synthase (ALS)-inhibiting herbicides of flixweed (Descurainia sophia L.) conferred by different combinations of ALS isozymes with a Pro-197-Thr mutation or a novel Trp-574-Leu mutation. Pestic Biochem Physiol 136:41–45 CrossRefGoogle ScholarPubMed
Fang, J, Zhang, Y, Liu, T, Yan, B, Li, J, Dong, L (2019) Target-site and metabolic resistance mechanisms to penoxsulam in barnyardgrass (Echinochloa crus-galli (L.) P. Beauv). J Agric Food Chem 67:8085–8095 CrossRefGoogle Scholar
Hada, Z, Menchari, Y, Rojano-Delgado, AM, Torra, J, Menéndez, J, Palma-Bautista, C, De Prado, R, Souissi, T (2021) Point mutations as main resistance mechanism together with P450-based metabolism confer broad resistance to different ALS-inhibiting herbicides in Glebionis coronaria from Tunisia. Front Plant Science 12:626702 CrossRefGoogle ScholarPubMed
Heap, I (2022) The International Herbicide-Resistant Weed Database. http://www.weedscience.org. Accessed: January 1, 2022Google Scholar
Huang, Z, Lu, Z, Huang, H, Li, W, Cao, Y, Wei, S (2021) Target site mutations and cytochrome P450s-involved metabolism confer resistance to nicosulfuron in green foxtail (Setaria viridis). Pestic Biochem Physiol 179:104956 CrossRefGoogle Scholar
Hulme, PE (2022) Hierarchical cluster analysis of herbicide modes of action reveals distinct classes of multiple resistance in weeds. Pest Manag Sci 78:1265–1271 CrossRefGoogle ScholarPubMed
Iwakami, S, Tanigaki, S, Uchino, A, Ozawa, Y, Tominaga, T, Wang, GX (2020) Characterization of the acetolactate synthase gene family in sensitive and resistant biotypes of two tetraploid Monochoria weeds, M. vaginalis and M. korsakowii . Pestic Biochem Physiol 165:104506 CrossRefGoogle ScholarPubMed
Jiang, YY, Chai, YP, Lu, MH, Han, XL, Lin, Q, Zhang, Y, Zhou, Y, Wang, XC, Gao, CX, Chen, QJ (2020) Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize. Genome Biol 21:1–10 CrossRefGoogle ScholarPubMed
Li, Z, Li, X, Chen, J, Peng, L, Wang, J, Cui, H (2020) Variation in mutations providing resistance to acetohydroxyacid synthase inhibitors in Cyperus difformis in China. Pestic Biochem Physiol 166:104571 CrossRefGoogle ScholarPubMed
Mei, Y, Si, C, Liu, M, Qiu, L, Zheng, MQ (2017) Investigation of resistance levels and mechanisms to nicosulfuron conferred by non-target-site mechanisms in large crabgrass (Digitaria sanguinalis L.) from China. Pestic Biochem Physiol 141:84–89 CrossRefGoogle ScholarPubMed
Perotti, VE, Larran, AS, Palmieri, VE, Martinatto, AK, Alvarez, CE, Tuesca, D, Permingeat, HR (2018) A novel triple amino acid substitution in the EPSPS found in a high-level glyphosate resistant Amaranthus hybridus population from Argentina. Pest Manag Sci 75:1242–1251 CrossRefGoogle Scholar
Seefeldt, SS, Jensen, JE, Fuerst, EP (1995) Log-logistic analysis of herbicide dose response relationships. Weed Technol 9:218–227 CrossRefGoogle Scholar
Sen, MK, Hamouzová, K, Mikulka, J, Bharati, R, Košnarová, P, Hamouz, P, Royc, A, Soukupa, J (2021) Enhanced metabolism and target gene overexpression confer resistance against acetolactate synthase-inhibiting herbicides in Bromus sterilis . Pest Manag Sci 77:2122–2128 CrossRefGoogle ScholarPubMed
Singh, Y, Singh, VP, Singh, G, Yadav, DS, Sinha, RKP, Johnson, DE, Mortimer, AM (2011) The implications of land preparation, crop establishment method and weed management on rice yield variation in the rice–wheat system in the Indo-Gangetic plains. Field Crop Res 121:64–74 CrossRefGoogle Scholar
Tanigaki, S, Uchino, A, Okawa, S, Miura, C, Hamamura, K, Matsuo, M, Yoshino, N, Ueno, N, Toyama, Y, Fukumi, N, Masuda, T, Shimono, Y, Tominaga, T, Iwakami, S (2021) Gene expression shapes the patterns of parallel evolution of herbicide resistance in the agricultural weed Monochoria vaginalis . New Phytol 232: 928–940 CrossRefGoogle ScholarPubMed
Tranel, PJ, Wright, TR (2002) Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci 50:700–712 CrossRefGoogle Scholar
Tranel, PJ, Wright, TR, Heap, IM (2021) Mutations in herbicide-resistant weeds to inhibition of acetolactate synthase. http://www.weedscience.org. Accessed: January 2, 2022Google Scholar
Uchino, A, Ogata, S, Kohara, H, Yoshida, S, Yoshioka, T, Watanabe, H (2007) Molecular basis of diverse responses to acetolactate synthase-inhibiting herbicides in sulfonylurea-resistant biotypes of Schoenoplectus juncoides . Weed Biol Manag 7:89–96 CrossRefGoogle Scholar
Walter, KL, Strachan, SD, Ferry, NM, Albert, HH, Castle, LA, Sebastian, SA (2014) Molecular and phenotypic characterization of Als1 and Als2 mutations conferring tolerance to acetolactate synthase herbicides in soybean. Pest Manag Sci 70:1831–1839 CrossRefGoogle Scholar
Wang, H, Zhang, L, Li, W, Bai, S, Zhang, X, Wu, C, Liu, W, Wang, J (2019) Isolation and expression of acetolactate synthase genes that have a rare mutation in shepherd’s purse (Capsella bursa-pastoris (L.) Medik.). Pestic Biochem Physiol 155:119–125 CrossRefGoogle Scholar
Wang, X, Xu, Q, Zhu, J, Liu, R, Wang, S, Liu, Y, Lu, Q, Wang, G (2013) Resistance comparison of Ammannia arenaria to bensulfuron-methyl in different paddy rice growing regions of Zhejiang Province. Chinese J Pestic Sci 15:52–58. ChineseGoogle Scholar
Xu, X, Liu, G, Chen, S, Li, B, Liu, X, Wang, X, Fan, C, Wang, G, Ni, H (2015) Mutation at residue 376 of AHAS confers tribenuron-methyl resistance in flixweed (Descurainia sophia) accession from Hebei Province, China. Pestic Biochem Physiol 125:62–68 CrossRefGoogle Scholar
Xu, Y, Xu, L, Shen, J, Li, X, Zheng, M (2021) Effects of a novel combination of two mutated acetolactate synthase (ALS) isozymes on resistance to ALS-inhibiting herbicides in flixweed (Descurainia sophia). Weed Sci 69:430–438 CrossRefGoogle Scholar
Yang, Q, Li, J, Shen, J, Xu, Y, Liu, H, Deng, W, Li, XF, Zheng, M (2018) Metabolic resistance to acetolactate synthase inhibiting herbicide tribenuron-methyl in Descurainia sophia L. mediated by cytochrome P450 enzymes. J Agric Food Chem 66:4319–4327 CrossRefGoogle Scholar
Yu, JL, McCullough, PK, McElroy, JS, Jespersen, D, Shilling, DG (2020) Gene expression and target-site mutations are associated with resistance to ALS inhibitors in annual sedge (Cyperus compressus) biotypes from Georgia. Weed Sci 68:460–466 CrossRefGoogle Scholar
Yu, Q, Jalaludin, A, Han, H, Chen, M, Sammons, RD, Powles, SB (2015) Evolution of a double amino acid substitution in the 5-enolpyruvylshikimate-3-phosphate synthase in Eleusine indica conferring high-level glyphosate resistance. Plant Physiol 167:1440–1447 CrossRefGoogle ScholarPubMed
Yu, Q, Powles, SB (2014) Resistance to AHAS inhibitor herbicides: current understanding. Pest Manag Sci 70:1340–1350 CrossRefGoogle ScholarPubMed
Zhang, J, Liu, B, Cai, X, Zhou, W, Wang, H, Lu, Q, Zhou, G, Liu, Y, Liang, W, Wang, S, Zhu, J (2020) Resistance and its resistant molecular mechanism of Ammannia arenaria to ALS inhibiting herbicides. Chinese J Pestic Sci 22:60–67. ChineseGoogle Scholar
Zhang, X, Wang, H, Bei, F, Wu, C, Zhang, L, Jia, S, Wang, J, Liu, W (2021) Investigating the mechanism of metabolic resistance to tribenuron-methyl in Capsella bursa-pastoris (L.) Medik. by full-length transcriptome assembly combined with RNA-Seq. J Agric Food Chem 69:3692–3701 CrossRefGoogle ScholarPubMed
Zhao, B, Fu, D, Yu, Y, Huang, C, Yan, K, Li, P, Shafi, J, Zhu, H, Wei, S, Ji, M (2017) Non-target-site resistance to ALS-inhibiting herbicides in a Sagittaria trifolia L. population. Pestic Biochem Physiol 140:79–84 CrossRefGoogle Scholar
Zhao, N, Yan, Y, Wang, H, Bai, S, Wang, Q, Liu, W, Wang, J (2018) Acetolactate synthase overexpression in mesosulfuron-methyl-resistant shortawn foxtail (Alopecurus aequalis Sobol.): reference gene selection and herbicide target gene expression analysis. J Agric Food Chem 66:9624–9634 CrossRefGoogle ScholarPubMed