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An Inexpensive Combustion Apparatus for Preparation of Radioactive Samples

Published online by Cambridge University Press:  12 June 2017

Dennis L. Bucholtz  and
F. Dan Hess
Dennis L. Bucholtz
Affiliation:
Dep. Bot. Plant Path., Purdue Univ., West Lafayette, IN 47907
F. Dan Hess
Affiliation:
Dep. Bot. Plant Path., Purdue Univ., West Lafayette, IN 47907
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Abstract

The combustion apparatus described provides a rapid and inexpensive alternative for measurement of radioisotopes in plant parts. The apparatus utilized a nickel-chromium resistance wire connected to a low-voltage transformer to generate the heat required to ignite the plant tissue. The nickel-chromium wire also served as the sample holder. Combustion was performed in an O2-flushed liquid scintillation vial, and the combustion gases containing either 14CO2 or 3H2O were trapped in situ, scintillant was added, and the vials assayed for radioactivity. The percentage of the radioactivity recovered after combustion was 96.0 for 14C and 93.4 for 3H with standard deviations of 6.8 and 3.0%, respectively.

Keywords

Plant tissue oxidizerradioactivity14carbontritiumliquid scintillation spectrometry
Type
Research Article
Information
Weed Science , Volume 31 , Issue 2 , March 1983 , pp. 159 - 162
Copyright
Copyright © 1983 Weed Science Society of America 

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References

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