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Amino Acid Substitutions in the Acetolactate Synthase Gene of Red Rice (Oryza sativa) Confer Resistance to Imazethapyr

Published online by Cambridge University Press:  20 January 2017

Marites A. Sales ,
Vinod K. Shivrain ,
Nilda R. Burgos  and
Yong I. Kuk
Marites A. Sales
Affiliation:
Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72704
Vinod K. Shivrain
Affiliation:
Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72704
Nilda R. Burgos*
Affiliation:
Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72704
Yong I. Kuk
Affiliation:
Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72704
*
Corresponding author's E-mail: nburgos@uark.edu.
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Abstract

Two red rice accessions from Arkansas have been found to be resistant to the labeled rate of imazethapyr, which is used to control red rice in ClearfieldTM rice. Full-length amplification of the acetolactate synthase (ALS) gene in imazethapyr-resistant red rice revealed a coding sequence of 1,935 base pairs, which is the same as that of the cultivated rice. Coding sequences were generated from four red rice accessions collected from different geographical regions in Arkansas, consisting of accessions that were either resistant or susceptible to imazethapyr. Nucleotide sequence alignments identified six base polymorphisms, three of which resulted in amino acid substitutions in the ALS gene. One amino acid substitution, Gly654Glu, involves a residue required for imazethapyr binding to the ALS. The other substitution, Val669Met, implies conformational changes in the ALS structure that enhances binding of thiamine diphosphate, an ALS cofactor. These novel amino acid substitutions first reported for ALS-resistant red rice accessions support the hypothesis that ALS-resistant red rice can evolve with sustained herbicide selection pressure. Thus, it behooves growers to integrate the Cleafield rice technology with other tools to achieve a successful, long-term weed management program.

Keywords

Imazethapyrred rice, Oryza sativa L.Acetolactate synthasecofactorsherbicide resistancethiamine diphosphate
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 56 , Issue 4 , August 2008 , pp. 485 - 489
Copyright
Copyright © Weed Science Society of America 

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