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Acetolactate Synthase–Inhibitor Resistance in Yellow Nutsedge (Cyperus esculentus): II—Physiognomy and Photoperiodic Response

  • Parsa Tehranchian (a1), Jason K. Norsworthy (a1), Muthukumar V. Bagavathiannan (a2) and Dilpreet S. Riar (a3)


Yellow nutsedge is one of the most problematic weedy sedges in rice–soybean systems of the Mississippi Delta region. An acetolactate synthase (ALS)-inhibiting, herbicide-resistant (Res) yellow nutsedge biotype was recently documented in eastern Arkansas, which showed intermediary growth habit between yellow nutsedge and purple nutsedge and also exhibited differential photoperiodic sensitivity to flowering. The objectives of this study were to: (a) determine variation in reproductive characteristics of the Res biotype and three susceptible (Sus) yellow nutsedge biotypes, (b) understand the influence of photoperiod on growth and reproduction, (c) understand the potential role of seeds in population establishment, and (d) elucidate the phylogenetic relationships between the Res yellow nutsedge biotype and purple nutsedge. Tuber production per plant and tuber weight of the Res biotype were less than that of the Sus biotypes. Differences in quantitative traits, such as shoot and tuber production existed between the Res and Sus biotypes for photoperiods ranging from 12 to 16 h. Generally, photoperiods greater than 12 h increased shoot development in all yellow nutsedge biotypes, with differential responses among the biotypes. Number of tubers reached the maximum for the Res biotype at a 14-h photoperiod. Over a 90-d period, inflorescence formation was only observed in the Res biotype with maximum flowering and seed production in the 14-h photoperiod. Subsequent tests revealed up to 18% seed germination, suggesting that seed could also play a role (in addition to tubers) in the persistence and spread of the Res yellow nutsedge. Phylogenetic analysis based on ribosomal DNA internal transcribed spacer (ITS) regions and mitochondrial nad4 gene intergenic spacer sequences indicated that the Res biotype was more closely associated with Sus yellow nutsedge biotypes. Nevertheless, 100% similarity for the nad4 gene sequences between the Res yellow nutsedge biotype and a reference purple nutsedge suggests that the Res biotype is likely a result of hybridization between yellow and purple nutsedges, which perhaps explains the intermediary growth characteristics observed in the Res biotype.

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Bagavathiannan, MV, Norsworthy, JK (2014) Pollen-mediated transfer of herbicide resistance in Echinochloa crus-galli . Pest Manag Sci 70:14251431
Bagavathiannan, MV, Norsworthy, JK, Tehranchian, P, Riar, DS (2015) ALS-inhibitor resistance in yellow nutsedge (Cyperus esculentus L.): I. Phenotypic differences. Weed Sci 63:810818
Baldwin, BG (1992) Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example for Compositae. Mol Phylogenet Evol 1:316
Bartlett, JR (1889) Dictionary of Americanisms: A Glossary of Words and Phrases Usually Regarded as Peculiar to the United States. 4th edn. Boston, MA Little, Brown and Co. 813 p
Box, EO (1996) Plant functional types and climate at the global scale. J Veg Sci 7:309320
Burke, IC, Holland, JB, Burton, JD, York, AC, Wilcut, JW (2007) Johnsongrass (Sorghum halepense) pollen expresses ACCase target-site resistance. Weed Technol 21:384388
Carstens, BC, Knowles, LL (2007) Estimating species phylogeny from gene-tree probabilities despite incomplete lineage-sorting: an example from Melanoplus grasshoppers. Syst Biol 56:400411
Catford, JA, Morris, WK, Vesk, PA, Gippel, CJ, Downes, B (2014) Species and environmental characteristics point to flow regulation and drought as drivers of riparian plant invasion. Divers Distrib 20:10841096
Chozin, MA, Yasuda, S (1991) Possibility of natural hybridization between Cyperus iria L. and Cyperus microiria Steud. Weed Res 36:282289
Christian, M, Qi, Y, Zhang, Y, Voytas, DF (2013) Targeted mutagenesis of Arabidopsis thaliana using engineering TAL effector nucleases. G3 (Bethesda) 3:16971705
Costa, J, Appleby, AP (1976) Response of two yellow nutsedge varieties to three herbicides. Weed Sci 24:5458
DeFelice, MS (2002) Yellow nutsedge Cyperus esculentus L. —snack food of the Gods. Weed Technol 16:901907
Demesure, B, Sodzi, N, Petit, RJ (1995) A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Mol Ecol 4:129131
Duminil, J, Pemonge, MH, Petit, RJ (2002) A set of 35 consensus primer pairs amplifying genes and introns of plant mitochondrial DNA. Mol Ecol Notes 2:428430
Garg, DK, Bendixen, LE, Anderson, SR (1967) Rhizome differentiation in yellow nutsedge. Weeds 15:124128
Hattori, N, Kitagawa, K, Takumi, S, Nakamura, C (2002) Mitochondrial DNA heteroplasmy in wheat, Aegilops and their nucleus-cytoplasm hybrids. Genetics 160:16191630
Holm, LG, Plucknett, JV, Pancho, JV, Herberger, JP (1991) The World's Worst Weeds Distribution and Biology Malabar FL: Kriegar Publishing. Pp 125133
Holt, J (1994) Genetic variation in life history traits in yellow nutsedge (Cyperus esculentus) from California. Weed Sci 42:378384
Hsiao, C, Jacobs, SWL, Chatterton, NJ, Asay, KH (1999) A molecular phylogeny of the grass family (Poaceae) based on the sequences of nuclear ribosomal DNA (ITS). Aust Syst Bot 11:667688
Jansen, LL (1971) Morphology and photoperiodic responses of yellow nutsedge. Weed Sci 19:210219
Koyama, T (1961) Classification of the family Cyperaceae (3). Q J Taiwan Mus 14:159194
Lapham, J, Drennan, DSH (1990) The fate of yellow nutsedge (Cyperus esculentus) seed and seedlings in soil. Weed Sci 38:125128
Manhart, JR (1989) Chemotaxonomy of the genus Carex (Cyperaceae). Can J Bot 68:14571461
Marienfeld, JR, Newton, KJ (1994) The nad4 gene of maize mitochondrial is highly conserved. Plant Physiol 104:301302
Marshall, DC, Hill, KBR, Cooley, JR, Simon, C (2011) Hybridization, mitochondrial DNA phylogeography, and prediction of the early stages of reproductive isolation: lessons from New Zealand Cicadas (Genus Kikihia). Systematic Biology 60:482502
Matthiesen, RL, Stoller, EW (1979) Tuber composition in yellow nutsedge (Cyperus esculentus (L.)) varieties. Weed Res 18:373377
McLain, DK, Wesson, DW, Collins, FH, Oliver, JH Jr. (1995) Evolution of the rDNA spacer, ITS2, in the ticks Lxodes scapularis and L. pacificus (Acari: Ixodidae). Heredity 75:303319
Michel, A, Arias, RS, Scheffler, BE, Duke, SO, Netherland, M, Dayan, FE (2004) Somatic mutation-mediated evolution of herbicide resistance in the nonindigenous invasive plant hydrilla (Hydrilla verticillata). Mol Ecol 13:32293237
Mulligan, GA, Junkins, BE (1976) The biology of Canadian weeds. 17. Cyperus esculentus L. Can J Plant Sci 56:339350
Norsworthy, JK, Bond, J, Scott, RC (2013) Weed management practices and needs in Arkansas and Mississippi rice. Weed Technol 27:623630
Okoli, CAN, Shilling, DG, Smith, RL, Bewick, TA (1997) Genetic diversity in purple nutsedge (Cyperus rotundus L.) and yellow nutsedge (Cyperus esculentus L.). Biol Control 8:111118
Ransom, CV, Rice, CA, Shock, CC (2009) Yellow nutsedge (Cyperus esculentus) growth and reproduction in response to nitrogen and irrigation. Weed Sci 57:2125
Ritland, CE, Ritland, K, Straus, NA (1993) Variation in ribosomal internal transcribed spacers (ITS1 and ITS2) among eight taxa of the Mimulus guttatus species complex. Mol Biol Evol 10:12731288
Schippers, P, Borg, SJT, Van Groenendael, JM, Habekotte, B (1993) What makes Cyperus esculentus (yellow nutsedge) an invasive species? A spatial model approach. Pages 495504 in Proceedings of the Brighton Crop Protection Conference—Weeds. Hampshire, UK British Crop Protection Council.
Schuster, W, Brennicke, A (1994) The plant mitochondrial genome: physical structure, information content, RNA editing, and gene migration to nucleus. Annu Rev Plant Physiol Plant Mol Biol 45:6178
Skuza, L, Rogalska, SM, Bocianowski, J (2007) RFLP analysis of mitochondrial DNA in the genus Secale. Acta Biol Cracov Ser Bot 49:7787
Sosnoskie, LM, Webster, TM, Kichler, JM, MacRae, AW, Grey, TL, Culpepper, AS (2012) Pollen-mediated dispersal of glyphosate-resistance in Palmer amaranth under field conditions. Weed Sci 60:366373
Stevens, OA (1932) The number and weight of seeds produced by weeds. Am J Bot 19:784794
Stoller, EW, Sweet, RD (1987) Biology and life cycle of purple and yellow nutsedge (Cyperus rotundus and C. esculentus). Weed Technol 1:6673
Tamura, K, Dudley, J, Nei, M, Kumar, S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:15961599
Tayyar, RI, Nguyen, JHT, Holt, JS (2003) Genetic and morphological analysis of two novel nutsedge biotypes from California. Weed Sci 51:731739
Tehranchian, P, Adair, RJ, Lawrie, AC (2014a) Potential for biological control of the weed angled onion (Allium triquetrum) by the fungus Stromatinia cepivora in Australia. Australas Plant Pathol 43:381392
Tehranchian, P, Norsworthy, JK, Nandula, V, McElroy, S, Chen, S, Scott, RC (2014b) First report of resistance to acetolactate synthase inhibiting herbicides in yellow nutsedge (Cyperus esculentus): confirmation and characterization. Pest Manag Sci. DOI: 10.1002/ps.3922
Thullen, RJ, Keeley, PE (1979) Seed production and germination in Cyperus esculentus and C. rotundus. Weed Sci 27:502505
Webster, TM (2005) Patch expansion of purple nutsedge (Cyperus rotundus) and yellow nutsedge (Cyperus esculentus) with and without polyethylene mulch. Weed Sci 53:839845
Went, FW (1957) The experimental control of plant growth. Waltham, MA Chronica Botanica Company. 343 p
White, TJ, Bruns, T, Lee, S, Taylor, J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pages 315322 in Innis Gelfand, M, Sninsky, D, White, T J, eds. PCR Protocols A Guide to Methods and Amplifications. San Diego, CA: Academic Press.



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