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Growth Response of Wheat (Triticum aestivum) Callus to Imazapyr and in Vitro Selection for Resistance

Published online by Cambridge University Press:  12 June 2017

David C. Heering
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078-0507
Arron C. Guenzi
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078-0507
Thomas F. Peeper
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078-0507
P. L. Claypool
Dep. Statistics, Oklahoma State Univ., Stillwater, OK 74078-0507


Intact wheat plants and wheat calli responded similarly to varying concentrations of imazapyr. Fifty percent growth inhibition of wheat callus occurred with 0.05 μM imazapyr after 70 d. As imazapyr concentration increased from 0 to 10 μM, the free isoleucine, leucine, and valine decreased from 160 to 35, 260 to 49, and 310 to 59 pmol mg−1, respectively. Resistant calli, which had relative growth rates exceeding a calculated upper prediction interval, were obtained by in vitro selection at 2 and 5 μM imazapyr. Resistant calli growing on 2 μM imazapyr had free isoleucine, leucine, and valine concentrations intermediate to the control and susceptible callus.

Physiology, Chemistry, and Biochemistry
Copyright © 1992 by the Weed Science Society of America 

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