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Acetolactate Synthase Gene Proline (197) Mutations Confer Tribenuron-Methyl Resistance in Flixweed (Descurainia sophia) Populations from China

Published online by Cambridge University Press:  20 January 2017

Hai Lan Cui
Affiliation:
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Key Laboratory of Weed and Rodent Biology and Management, and State Key Laboratory for Biology of Plant Diseases and Insect Pests, No. 2 West Yuanmingyuan Road, Haidian, Beijing 100193, China
Chao Xian Zhang*
Affiliation:
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Key Laboratory of Weed and Rodent Biology and Management, and State Key Laboratory for Biology of Plant Diseases and Insect Pests, No. 2 West Yuanmingyuan Road, Haidian, Beijing 100193, China
Shou Hui Wei
Affiliation:
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Key Laboratory of Weed and Rodent Biology and Management, and State Key Laboratory for Biology of Plant Diseases and Insect Pests, No. 2 West Yuanmingyuan Road, Haidian, Beijing 100193, China
Hong Jun Zhang
Affiliation:
Institute for the Control of Agrochemicals, Ministry of Agriculture, China, No. 22 Maizidian Street, Chaoyang, Beijing 100125, China
Xiang Ju Li
Affiliation:
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Key Laboratory of Weed and Rodent Biology and Management, and State Key Laboratory for Biology of Plant Diseases and Insect Pests, No. 2 West Yuanmingyuan Road, Haidian, Beijing 100193, China
Yan Qiu Zhang
Affiliation:
Institute for the Control of Agrochemicals, Ministry of Agriculture, China, No. 22 Maizidian Street, Chaoyang, Beijing 100125, China
Gui Qi Wang
Affiliation:
Institute of Food and Oil, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031, China
*
Corresponding author's E-mail: cxzhang@wssc.org.cn

Abstract

The molecular basis of resistance to tribenuron-methyl, an acetolactate synthase (ALS)–inhibiting herbicide was investigated in four resistant (R) and three susceptible (S) flixweed populations. The resistance level in the R populations was assessed in whole-plant pot experiments in a greenhouse, and resistance indices ranged from 723 to 1422. The ALS genes of the three S populations and four R populations were cloned and sequenced, and the full coding sequence of the ALS gene of flixweed was 2,004 bp. The sequences of the ALS genes of the three S populations collected from Shaanxi, Gansu, and Tianjin were identical. Comparison of the ALS gene sequences of the S and R populations with Arabidopsis revealed that proline at position 197 of the ALS gene was substituted by leucine in R population SSX-2, by alanine in R population SSX-3, and by serine in R populations TJ-2 and GS-2. In another study of two R flixweed populations from Hebei and Shaanxi, resistance was also related to mutation at position 197 of the ALS gene. Both studies confirmed tribenuron-methyl resistance in flixweed in China, with the resistance mechanism being conferred by specific ALS point mutations at amino acid position 197.

Type
Weed Management
Copyright
Copyright © Weed Science Society of America 

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