Hostname: page-component-8448b6f56d-c4f8m Total loading time: 0 Render date: 2024-04-23T10:05:13.277Z Has data issue: false hasContentIssue false
  • English
  • Français

Müller cells in the retina of the cane toad, Bufo marinus, express neuropeptide Y-like immunoreactivity

Published online by Cambridge University Press:  02 June 2009

Bao-Song Zhu  and
Ian Gibbins
Bao-Song Zhu
Affiliation:
Department of Anatomy and Histology and Centre for Neuroscience, School of Medicine, The Flinders University of South Australia, Adelaide, Australia
Ian Gibbins
Affiliation:
Department of Anatomy and Histology and Centre for Neuroscience, School of Medicine, The Flinders University of South Australia, Adelaide, Australia
Get access Rights & Permissions [Opens in a new window]

Abstract

We have used light- and electron-microscopic immunohistochemistry to identify the presence of immunoreactivity to neuropeptide Y (NPY) within Müller cells in the retina of the cane toad, Bufo marinus. Müller cells containing NPY-like immunoreactivity (NPY-LI) were identified at the light-microscopic level by the coexistence with immunoreactivity to glial fibrillary acidic protein (GFAP) and at the ultrastructural level by their characteristic relationship to neuron cell bodies and processes. At the light-microscopic level, those cells which contained both NPY-LI and GFAP-LI usually had small cell bodies in the inner nuclear layer, while those cells which contained only NPY-LI were identified as large and small amacrine cells. The radially oriented primary processes in the inner plexiform layer and the vitreal end feet of GFAP-LI Müller cells also expressed NPY-LI. At the ultrastructural level, thin lamellar processes of Müller cells with NPY-LI enclosed some amacrine cell bodies in the inner nuclear layer and amacrine cell dendrites in the inner plexiform layer. These observations suggest that NPY-LI is localized in Müller cells in addition to two types of amacrine cells previously identified in the Bufo retina. This study provides the first evidence that glial elements in the vertebrate retina express NPY-LI.

Keywords

GliaMüller cellsNeuropeptide YRetinaAmphibian
Type
Research Articles
Information
Visual Neuroscience , Volume 13 , Issue 3 , May 1996 , pp. 501 - 508
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Blankenfeld, G. Von & Kettenmann, H. (1991). Glutamate and GABA receptors in vertebrate glial cells. Molecular Neurobiology 5, 31–43.Google Scholar
Blessing, W.W., Howe, P.R.C., Joh, T.H., Oliver, J.R. & Willoughby, J.O. (1986). Distribution of tyrosine hydroxylase and neuropeptide Y-like immunoreactive neurons in rabbit medulla oblongata, with attention to colocalization studies, presumptive adrenaline-synthesizing perikarya, and vagal preganglionic cells. Journal of Comparative Neurology 248, 285–300.CrossRefGoogle ScholarPubMed
Bruun, A. & Ehinger, B. (1993). NPY-induced neurotransmitter release from the rabbit and chicken retina. Ada Ophthalmologies (Copenhagen) 71, 590–596.Google ScholarPubMed
Chartrel, N., Conlon, J.M., Dancer, J.-M., Fournier, A., Tonon, M.-C. & Vaudry, H. (1991). Characterization of melanotropin-release-inhibiting factor (melanostatin) from frog brain: Homology with human neuropeptide Y. Proceedings of the National Academy of Sciences of the U.S.A. 88, 3862–3866.CrossRefGoogle ScholarPubMed
Gábriel, R., Wilhelm, M. & Straznicky, C. (1993). Morphology and distribution of Müller cells in the retina of the toad Bufo marinus. Cell and Tissue Research 272, 183–192.CrossRefGoogle ScholarPubMed
Girard, A., Crisanti-Combes, P., Faivre-Bauman, A., Dubois, M.P. & Pessac, B. (1981). Demonstration of neuropeptides in cultured neuroretinal cells of chick embryo. Comptes Rendus des Seances de 1'Academic des Sciences, Serie II 293, 291–295.Google ScholarPubMed
Griffin, D., Minth, C.D. & Taylor, W.L. (1994). Isolation and characterization of the Xenopus laevis cDNA and genomic homologs of neuropeptide Y. Molecular and Cellular Endocrinology 101, 1–10.CrossRefGoogle ScholarPubMed
Hiscock, J. & Straznicky, C. (1989). Neuropeptide Y-like immunoreactive amacrine cells in the retina of Bufo marinus. Brain Research 494, 55–64.CrossRefGoogle ScholarPubMed
Hösli, E. & Hösli, L. (1993). Autoradiographic localization of binding sites for neuropeptide Y and bradykinin on astrocytes. Neuroreport 4, 159–162.CrossRefGoogle ScholarPubMed
Kanai, Y., Smith, C.P. & Hediger, M.A. (1993). A new family of neurotransmitter transporters: The high-affinity glutamate transporters. FASEB Journal 7, 1450–1459.CrossRefGoogle ScholarPubMed
Larhammar, D., Söderberg, C. & Blomqvist, A.G. (1993 a). Evolution of the neuropeptide Y family of peptides. In The Neurobiology of Neuropeptide Y and Related Peptides, ed. Colmers, W.F. & Wahlestedt, C., pp. 1–42. Totowa: Humana Press.Google Scholar
Larhammar, D., Blomqvist, A.G. & Söderberg, C. (1993 b). Evolution of neuropeptide Y and its related peptides. Comparative Biochemistry and Physiology 106, 743–752.Google ScholarPubMed
Lucius, R. & Mentlein, R. (1991). Degradation of the neuropeptide somatostatin by cultivated neuronal and glial cells. Journal of Biological Chemistry 266, 18907–18913.CrossRefGoogle ScholarPubMed
Maccarrone, C. & Jarrott, B. (1985). Differences in regional brain concentrations of neuropeptide Y in spontaneously hypertensive (SH) and Wistar-Kyoto (WKY) rats. Brain Research 345, 165–169.Google Scholar
Malchow, R.P., Gian, H. & Ripps, H. (1989). γ-Aminobutyric acid (GABA)-induced currents of skate Müller (glial) cells are mediated by neuronal-like GABAA receptors. Proceedings of the National Academy of Sciences of the U.S.A. 86, 4326–4330.CrossRefGoogle ScholarPubMed
Martin, D.L. (1992). Synthesis and release of neuroactive substances by glial cells. Clio 5, 81–94.Google ScholarPubMed
Masters, B.A., Pruysers, C.R., Millard, W.J., Meyer, E.M. & Poulkos, J.J. (1990). Preproneuropeptide Y mRNA expression in glial cell cultures of the neonate but not 21-day-old rat brain. Annals of the New York Academy of Sciences 611, 522–524.CrossRefGoogle Scholar
McKay, D.M., Shaw, C., Halton, D.W., Thim, L. & Buchanan, K.D. (1992). The primary structure and tissue distribution of an amphibian neuropeptide Y. Regulatory Peptides 37, 143–153.CrossRefGoogle ScholarPubMed
Morris, J.L., Gibbins, I.L., Campbell, G., Murphy, R., Furness, J.B. & Costa, M. (1986). Innervation of the large arteries and heart of the toad Bufo marinus by adrenergic and peptide-containing neurons. Cell and Tissue Research 243, 171–184.Google Scholar
Sassoè, Pognetto M., Panzanelli, P., Artero, C., Fasolo, A. & Cantino, D. (1992). Comparative study of glial fibrillary acidic protein (GFAP)-like immunoreactivity in the retina of some representative vertebrates. European Journal of Hislochemistry 36, 467–477.Google Scholar
Sato, K., Yoshida, S., Fujiwara, K., Tada, K. & Tohyama, M. (1991). Glycine cleavage system in astrocytes. Brain Research 567, 64–70.CrossRefGoogle ScholarPubMed
Spruce, B.A., Curtis, R., Wilkin, G.P. & Glover, D.M. (1990). A neuropeptide precursor in cerebellum: Proenkephalin exists in sub-populations of both neurons and astrocytes. EMBO Journal 9, 1787–1795.CrossRefGoogle Scholar
Van Riel, M.C.H.M., Tuinhof, R., Roubos, E.W. & Martens, G.J.M. (1993). Cloning and sequence analysis of hypothalamic cDNA encoding Xenopus preproneuropeptide Y. Biochemical and Biophysical Research Communications 190, 948–951.CrossRefGoogle ScholarPubMed
Vernadakis, A. (1988). Neuron-glia interrelations. International Review of Neurobiology 30, 149–224.Google Scholar
Wilhelm, M., Straznicky, C. & Gábriel, R. (1992). Neuron-specific enolase-like immunoreactivity in the vertebrate retina: Selective labelling of Müller cells in anura. Hislochemistry 98, 243–252.CrossRefGoogle ScholarPubMed
Zhu, B.-S. & Gibbins, I. (1995). Synaptic circuitry of neuropeptide-containing amacrine cells in the retina of the cane toad, Bufo marinus. Visual Neuroscience 12, 919–927.CrossRefGoogle ScholarPubMed
Zhu, B.-S., Gábriel, R. & Straznicky, C. (1992). Serotonin synthesis and accumulation by neurons of the anuran retina. Visual Neuroscience 9, 377–388.CrossRefGoogle ScholarPubMed