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Protein Kinase C-like immunoreactivity in rod bipolar cells of the rat retina: A developmental study

Published online by Cambridge University Press:  02 June 2009

Danru Zhang  and
Hermes H. Yeh
Danru Zhang
Affiliation:
Department of Neurobiology and Anatomy, University of Rochester Medical Center, Rochester
Hermes H. Yeh
Affiliation:
Department of Neurobiology and Anatomy, University of Rochester Medical Center, Rochester
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Abstract

In the retina of a variety of vertebrate species, a monoclonal antibody against protein kinase C (PKC) has been shown to label preferentially bipolar cells. Although the functional consequences of PKC activation in these cells is yet to be revealed, the present study was motivated in part by the possibility that the antibody might be used as a selective marker for examining the development of bipolar cells in the rat retina. Here, the developmental pattern and the dynamic changes of retinal cells expressing PKC-like immunoreactivity (PKC-LI) were studied and analyzed throughout postnatal life until adulthood. Upon its initial detection by immunohistochemistry on postnatal day (PD)-10, faint PKC-LI was limited to the central region of the retina, labeling cell bodies located at the scleral margin of the inner nuclear layer (INL) adjacent to the outer plexiform layer (OPL). On subsequent days, PKC-LI spread progressively to the peripheral retina and axon terminal bulbs at the vitreal margin of theinner plexiform layer (IPL) began showing the first signs of immunoreactive labeling. Not until PD-15, the time of eye opening, did PKC-LI in these cells increase to the extent such that their thin axons were immunoreactive. Each of these axons traversed the entire thickness of the IPL and divided into two or three short branches before ending as enlarged terminal bulbs. The morphology and the location of PKC-LI cells in both the developing and adult retina observed in our study are consistent with them being rod bipolar cells. By the end of the fourth postnatal week, the rod bipolar cells appeared mature, resembling those found in the adult. Overall, more dynamic changes occurred at the axon terminal bulbs than at the cell bodies during the maturational process of rod bipolar cells. Interestingly, PKIC-LI was expressed precociusly in these cells when rat pups were reared in complete darkness starting from the day of birth.

Keywords

Rat retinaProtein kinase CBipolar cellsPostnatal developmentImmunohistochemistry
Type
Research Articles
Information
Visual Neuroscience , Volume 6 , Issue 5 , May 1991 , pp. 429 - 437
Copyright
Copyright © Cambridge University Press 1991

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