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Heritability and Whole Genome Linkage of Pulse Pressure in Chinese Twin Pairs

  • Wengjie Jiang (a1), Dongfeng Zhang (a1), Zengchang Pang (a1) (a2), Shuxia Li (a3), Haiping Duan (a2), Shaojie Wang (a2) and Qihua Tan (a4) (a3)...


Elevated pulse pressure is associated with cardiovascular disorders and mortality in various populations. The genetic influence on pulse pressure has been confirmed by heritability estimates using related individuals. Recently, efforts have been made in mapping genes that are linked to the phenotype. We report results on our heritability and linkage study conducted on the Chinese population in mainland China where cardiovascular and cerebrovascular diseases are becoming the leading cause of death. A total of 630 pairs of middle-aged Chinese twins were collected for heritability analysis, from which 63 dizygotic twin pairs were randomly selected for genome-wide linkage analysis using Affymetrix 6.0 SNP array. Regression analysis reconfirmed the significant effects of age, sex, and BMI on pulse pressure. Comparison of twin models suggested the parsimonious AE model as the best model with a heritability estimate of 0.45. Genome-wide non-parametric linkage analysis identified three significant linkage peaks on chromosome 11 (lod score 4.06 at 30.5 cM), chromosome 12 (lod score 3.97 at 100.7 cM), and chromosome 18 (lod score 4.01 at 70.7 cM) with the last two peaks closely overlapping with linkage peaks reported by two American studies. In addition, multiple regions with suggestive linkage were identified with many of them overlapping with published linkage regions. Our results provide both epidemiological and molecular genetic evidence for the genetic dissection of pulse pressure in the Chinese population, which could help in fine mapping and in characterizing genes that are involved in the regulation of pulse pressure.

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Corresponding author

address for correspondence: Wenjie Jiang, Department of Public Health, Qingdao University Medical College, Deng Zhou Street 38, 266021 Qingdao, China. E-mail:


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Abecasis, G. R., Cherny, S. S., Cookson, W. O., & Cardon, L. R. (2002). Merlin — Rapid analysis of dense genetic maps using sparse gene flow trees. Nature Genetics, 30, 97101.
Amos, C. I. (1994). Robust variance-components approach for assessing genetic linkage in pedigrees. American Journal of Human Genetics, 54, 535543.
Atwood, L. D., Samollow, P. B., Hixson, J. E., Stern, M. P., & MacCluer, J. W. (2001). Genome-wide linkage analysis of blood pressure in Mexican Americans. Genetic Epidemiology, 20, 373382.
Bielinski, S. J., Lynch, A. I., Miller, M. B., Weder, A., Cooper, R., Oberman, A., Chen, Y. D., Turner, S. T., Fornage, M., Province, M., & Arnett, D. K. (2005). Genome-wide linkage analysis for loci affecting pulse pressure: The Family Blood Pressure Program. Hypertension, 46, 12861293.
Bochud, M., Bovet, P., Elston, R. C., Paccaud, F., Falconnet, C., Maillard, M., Shamlaye, C., & Burnier, M. (2005). High heritability of ambulatory blood pressure in families of East African descent. Hypertension, 45, 445450.
Camp, N. J., Hopkins, P. N., Hasstedt, S. J., Coon, H., Malhotra, A., Cawthon, R. M., & Hunt, S. C. (2003). Genome-wide multipoint parametric linkage analysis of pulse pressure in large, extended utah pedigrees. Hypertension, 42, 322328.
Cho, K., & Dupuis, J. (2009). Handling linkage disequilibrium in qualitative trait linkage analysis using dense SNPs: A two-step strategy. BMC Genetics, 10, 44.
Dart, A. M., & Kingwell, B. A. (2001). Pulse pressure — A review of mechanisms and clinical relevance. Journal of the American College of Cardiology, 37, 975984.
DeStefano, A. L., Larson, M. G., Mitchell, G. F., Benjamin, E. J., Vasan, R. S., Li, J., Corey, D., & Levy, D. (2004). Genome-wide scan for pulse pressure in the National Heart, Lung and Blood Institute's Framingham Heart Study. Hypertension, 44, 152155.
Evans, D. M., & Cardon, L. R. (2004). Guidelines for genotyping in genomewide linkage studies: Single-Nucleotide-Polymorphism maps versus microsatellite maps. American Journal of Human Genetics, 75, 687692.
Franceschini, N., MacCluer, J. W., Rose, K. M., Rutherford, S., Cole, S. A., Laston, S., Göring, H. H., Diego, V. P., Roman, M. J., Lee, E. T., Best, L. G., Howard, B. V., Fabsitz, R. R., & North, K. E. (2008). Genome-wide linkage analysis of pulse pressure in American Indians: The Strong Heart Study. American Journal of Hypertension, 21, 194199.
Franklin, S. S., Gustin, W., Wong, N. D., Larson, M. G., Weber, M. A., Kannel, W. B., & Levy, D. (1997). Hemodynamic patterns of age-related changes in blood pressure: The Framingham heart study. Circulation, 96, 308315.
Lander, E., & Kruglyak, L. (1995). Genetic dissection of complex traits: Guidelines for interpreting and reporting linkage results. Nature Genetics, 11, 241247.
London, G. M., Guerin, A. P., Pannier, B., Marchais, S. J., & Stimpel, M. (1995). Influence of sex on arterial hemodynamics and blood pressure: Role of body height. Hypertension, 26, 514519.
Marlow, A. J. (2002). Nonparametric linkage analysis. II. Variance components. Methods in Molecular Biology, 195, 61100.
McGraw, K. O., & Wong, S. P. (1996). Forming inferences about some intraclass correlation coefficients. Psychological Methods, 1, 3046.
Mitchell, G. F., DeStefano, A. L., Larson, M. G., Benjamin, E. J., Chen, M. H., Vasan, R. S., Vita, J. A., & Levy, D. (2005). Heritability and a genome-wide linkage scan for arterial stiffness, wave reflection, and mean arterial pressure: The Framingham Heart Study. Circulation, 112, 194199.
Neale, M. C., Boker, S. M., Xie, G., & Maes, H. (2003). Mx: Statistical Modeling (6th ed.). Richmond, VA: VCU Department of Psychiatry.
Pilia, G., Chen, W. M., Scuteri, A., Orrú, M., Albai, G., Dei, M., Lai, S., Usala, G., Lai, M., Loi, P., Mameli, C., Vacca, L., Deiana, M., Olla, N., Masala, M., Cao, A., Najjar, S. S., Terracciano, A., Nedorezov, T., Sharov, A., Zonderman, A. B., Abecasis, G. R., Costa, P., Lakatta, E., & Schlessinger, D. (2006). Heritability of cardiovascular and personality traits in 6,148 Sardinians. PLoS Genetics, 2, e132.
Rijsdijk, F. V., & Sham, P. C. (2002). Analytic approaches to twin data using structural equation models. Briefings in Bioinformatics, 3, 119133.
Schaid, D. J., Guenther, J. C., Christensen, G. B., Hebbring, S., Rosenow, C., Hilker, C. A., McDonnell, S. K., Cunningham, J. M., Slager, S. L., Blute, M. L., & Thibodeau, S. N. (2004). Comparison of microsatellites versus single-nucleotide polymorphisms in a genome linkage screen for prostate cancer-susceptibility Loci. American Journal of Human Genetics, 75, 948965.
Sherva, R., Miller, M. B., Lynch, A. I., Devereux, R. B., Rao, D. C., Oberman, A., Hopkins, P. N., Kitzman, D. W., Atwood, L. D., & Arnett, D. K. (2007). A whole genome scan for pulse pressure/stroke volume ratio in African Americans: The HyperGEN study. American Journal of Hypertension, 20, 398402.
Skurnick, J. H., Aladjem, M., & Aviv, A. (2010). Sex differences in pulse pressure trends with age are cross-cultural. Hypertension, 55, 4047.
Smulyan, H., Marchais, S. J., Pannier, B., Guerin, A. P., Safar, M. E., & London, G. M. (1998). Influence of body height on pulsatile arterial hemodynamic data. Journal of the American College of Cardiology, 31, 11031109.
Snieder, H., Hayward, C. S., Perks, U., Kelly, R. P., Kelly, P. J., & Spector, T. D. (2000). Heritability of central systolic pressure augmentation: A twin study. Hypertension, 35, 574579.
Turner, S. T., & Boerwinkle, E. (2003). Genetics of blood pressure, hypertensive complications, and antihypertensive drug responses. Pharmacogenomics, 4, 5365.
van Rijn, M. J., Schut, A. F., Aulchenko, Y. S., Deinum, J., Sayed-Tabatabaei, F. A., Yazdanpanah, M., Isaacs, A., Axenovich, T. I., Zorkoltseva, I. V., Zillikens, M. C., Pols, H. A., Witteman, J. C., Oostra, B. A., & van Duijn, C. M. (2007). Heritability of blood pressure traits and the genetic contribution to blood pressure variance explained by four blood-pressure-related genes. Journal of Hypertension, 25, 565570.
Zeger, S. L., & Liang, K. Y. (1986). Longitudinal data analysis for discrete and continuous outcomes. Biometrics, 42, 121130.
Zhao, L., Stamler, J., Yan, L. L., Zhou, B., Wu, Y., Liu, K., Daviglus, M. L., Dennis, B. H., Elliott, P., Ueshima, H., Yang, J., Zhu, L., Guo, D., & INTERMAP Research Group (2004). Blood pressure differences between northern and southern Chinese: Role of dietary factors: The International Study on Macronutrients and Blood Pressure. Hypertension, 43, 13321337.
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Twin Research and Human Genetics
  • ISSN: 1832-4274
  • EISSN: 1839-2628
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