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Diagnosing Zygosity in Giant Panda Twins Using Short Tandem Repeats

  • Maiju Qiao (a1) (a2), Yingmin Zhou (a2), Thomas Connor (a3), Rengui Li (a2), Dan Tang (a2), Hemin Zhang (a2) and Jianghong Ran (a1)...

Abstract

The giant panda, native to mountains of south-west China, is one of the world's rarest bear species and is subject to considerable conservation effort. In captivity, the proportion of twins accounts for 54% of the total number of births. To date, little is known about zygosity in panda populations — specifically, the proportion of monozygotic and dizygotic twins. In this study, we used 10 microsatellite markers for reliable zygosity testing, and the probability of monozygotic twins was 99.963% when all 10 markers were concordant. Out of 43 studied twin pairs, no MZ twins were found, indicating that there may be no identical panda twins (or the incidence is very low). We speculate that the fertilized eggs of giant pandas do not have the capability to split into two identical embryos, or that this ability is very poor, which is likely due to delayed implantation that is common in bear species. The results of this study deepen our understanding of giant panda breeding, yield insight into panda twins’ likely mechanism of formation, and reduce the uncertainty of individual identity in wild population surveys.

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Corresponding author

address for correspondence: Jianghong Ran, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China. E-mail: ranjianghong@scu.edu.cn

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These authors contributed equally to this study.

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References

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Becker, A., Busjahn, A., Faulhaber, H. D., Bähring, S., Robertson, J., Schuster, H., & Luft, F. C. (1997). Twin zygosity. Automated determination with microsatellites. Journal of Reproductive Medicine, 42, 260266.
Bortolus, R., Parazzini, F., Chatenoud, L., Benzi, G., Bianchi, M. M., & Marini, A. (1999). The epidemiology of multiple births. Human Reproduction Update, 5, 179187.
Cutler, T. L., Murphy, K., Hopper, J. L., Keogh, L. A., Dai, Y., & Craig, J. M. (2015). Why accurate knowledge of zygosity is important to twins. Twin Research & Human Genetics, 18, 298305.
Derom, R., Orlebeke, J., Eriksson, A., & Thiery, M. (1995). The epidemiology of multiple births in Europe. In Keith, L. G., Papiernik, E., Keith, D. M. & Luke, B. (Eds.), Multiple pregnancy (pp. 145162). London: The Parthenon Publishing Group.
Forget-Dubois, N., Pérusse, D., Turecki, G., Girard, A., Billette, J. M., Rouleau, G., . . . Tremblay, R. E. (2003). Diagnosing zygosity in infant twins: Physical similarity, genotyping, and chorionicity. Twin Research, 6, 479485.
He, X., Zhu, D., Han, Z., Liu, X., Wang, G., Chu, S., . . . Huang, W. (2001). Use of microsatellite in zygosity diagnosis of twins. Chinese Journal of Medical Genetics, 18, 421425. (In Chinese)
Huang, J., Li, Y. Z., Du, L. M., Yang, B., Shen, F. J., Zhang, H. M., . . . Yue, B. S. (2015). Genome-wide survey and analysis of microsatellites in giant panda (Ailuropoda melanoleuca), with a focus on the applications of a novel microsatellite marker system. BMC Genomics, 16, 6172.
Jackson, R. W., Snieder, H., Davis, H., & Treiber, F. A. (2001). Determination of twin zygosity: A comparison of DNA with various questionnaire indices. Twin Research, 4, 1218.
Marshall, T. C., Slate, J., Kruuk, L. E., & Pemberton, J. M. (1998). Statistical confidence for likelihood-based paternity inference in natural populations. Molecular Ecology, 7, 639655.
Oleszczuk, J. J., Keith, D. M., Keith, L. G., & Rayburn, W. F. (1999). Projections of population-based twinning rates through the year 2100. Journal of Reproductive Medicine, 44, 913921.
Raymond, M., & Rousset, F. (1995). GENEPOP (version 1.2): Population genetics software for exact tests and ecumenicism. Journal of Heredity, 86, 248249.
Rousset, F. (2008). Genepop'007: A complete reimplementation of the Genepop software for windows and Linux. Molecular Ecology Resources, 8, 103106.
Shan, L., Hu, Y., Zhu, L., Yan, L., Wang, C., Li, D., . . . Wei, F. (2014). Large-scale genetic survey provides insights into the captive management and reintroduction of giant pandas. Molecular Biology Evolution, 31, 26632671.
Smits, J., & Monden, C. (2011). Twinning across the developing world. PLoS One, 6, e25239.
State Council Information Office. (2015). The State Forestry Administration held a press conference for the fourth national giant panda survey results (in Chinese). Retrieved from http://www.scio.gov.cn/xwfbh/gbwxwfbh/fbh/Document/1395514/1395514.htm
Sutherland-Smith, M., Morris, P. J., & Silverman, S. (2004). Pregnancy detection and fetal monitoring via ultrasound in a giant panda (Ailuropoda melanoleuca). Zoo Biology, 23, 449461.
Waits, L. P., Luikart, G., & Taberlet, P. (2001). Estimating the probability of identity among genotypes in natural populations: Cautions and guidelines. Molecular Ecology, 10, 249256.
Wang, H., Hou, R., Liu, Y., Cai, Z., Luo, L., Huang, H., & Zhang, Z. (2015). An observational analysis of relevant factors on twin rate of giant panda. Sichuan Journal of Zoology, 34, 650655. (In Chinese)
Wei, F., Hu, Y., Zhu, L., Bruford, M. W., Zhan, X., & Zhang, L. (2012). Black and white and read all over: The past, present and future of giant panda genetics. Molecular Ecology, 21, 56605674.
Zhang, H., Li, D., Wang, C., & Hull, V. (2009). Delayed implantation in giant pandas: The first comprehensive empirical evidence. Reproduction, 138, 979986.
Zhang, Z., Zhang, A., Hou, R., Wang, J., Li, G., Fei, L., . . . Maple, T. L. (2006). Historical perspective of breeding giant pandas ex situ in China and high priorities for the future. Cambridge, UK: Cambridge University Press.

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